Comparison of various techniques for the extraction and determination of antioxidants in plants

Abstract

The following extraction techniques have been used for extracting antioxidants (apigenin, coumarin, esculetin, umbelliferone, bergapten, quercetin, rutin, scopoletin and xanthotoxin) from plant material: supercritical fluid extraction, pressurized liquid extraction, extraction by means of Soxhlet apparatus, ultrasonic extraction in ultrasonic bath, and by means of ultrasonic probe. The analytical method based on HPLC-UV detection for the determination of selected antioxidants was developed. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical was also determined. Comparing all results the ultrasonic probe method using 0.75 g of sample extracted by 50 mL of acetonitrile in water (30%, v/v) for 25 min at room temperature and with amplitude at 60% (equal to 90 W) without pulsation was evaluated as the best tool. The most significant indicator demonstrating this statement is the antioxidant capacity expressed as gallic acid equivalent where the ultrasonic probe method showed the best results in 10 of 16 samples. Also the operability of ultrasonic probe extraction method compared to other tested methods is more favorable.

Extraction of rosmarinic acid from Melissa officinalis L. by heat-, microwave- and ultrasound-assisted extraction techniques: A comparative study through response surface analysis

“…The most relevant are supercritical carbon dioxide extraction, liquid CO 2 extraction, UAE, MAE and HAE [38][39][40]. There are few works that compare the conventional and alternative extraction techniques [10,17,35].…”

2-Oxoglutarate-dependent dioxygenases in the biosynthesis of simple coumarins

“…These molecules exhibit various biological activities such as antibacterial (Schinkovitz et al, 2003; Stavri et al, 2003; Céspedes et al, 2006), antioxidant (Bajerova et al, 2014), anti-inflammatory (Witaicenis et al, 2013), rodenticidal (Lotfi et al, 1996), termiticidal (Adfa et al, 2010, 2011), and other activities (Stahmann et al, 1941; Murray, 1989; Runkel et al, 1996; Song et al, 2014). In addition, the role(s) of coumarins in plants have also been reported.…”

Influence of different extraction methods on the quantification of selected flavonoids and phenolic acids from Tilia cordata inflorescence

“…The comparison between USAE, and different extraction method developed by other authors, showed the superiority of ultrasound assisted extraction for extracting phenolic compounds for example from Phlomidoschema parviflorum L. leaves (Majd et al, 2014), Thymus vulgaris L. herb, Verbena officinalis L. flovers (Bajerová et al, 2014), Vitis vinifera L. seeds (Da Porto et al, 2013), Ceratonia siliqua (Roseiro et al, 2013) and Betula alleghaniensis (Diouf et al, 2009).…”