Comparison of Various Solutions for Temporary Storage of Umbilical Cord Derived Mesenchymal Stem Cells

Krishnanda, Stanislaus Ivanovich; Agarwal, Raksheeth; Yausep, Oliver Emmanuel; Rizkita, Muthia; Angraeni, Ria; Pawitan, Jeanne Adiwinata

doi:10.9734/arrb/2017/38233

Cited by 4 publications

(5 citation statements)

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“…Moreover, PDT in DMEM-HG could not be assessed due to failure to attach and proliferate, while in physiologic saline and PBS the cells still proliferate though the PDT increased considerably. We supposed that after 168 hours, there was exhaustion of cell metabolism due to the high glucose content (5). Therefore, in this study we changed DMEM-HG to DMEM, but the result of this study showed that DMEM was inferior to physiologic saline, after 24 hours.…”

Section: Discussionmentioning

confidence: 92%

“…Our previous study on umbilical cord MCSs (UC-MSCs), which compared physiologic saline, phosphate buffered saline (PBS) and high glucose DMEM (DMEM-HG) showed that viability was >70% up to 72 hours in physiologic saline, and up to 96 hours in DMEM-HG, so the best storage solution up to 96 hours was DMEM-HG. However, after 168 hours, viability in DMEM-HG dropped considerably and was below the viability in physiologic saline (5). Moreover, PDT in DMEM-HG could not be assessed due to failure to attach and proliferate, while in physiologic saline and PBS the cells still proliferate though the PDT increased considerably.…”

Section: Discussionmentioning

confidence: 95%

“…Before administration to patients, MSCs are usually suspended in physiologic saline. However, MSCs only survive for a limited time in physiologic saline (5). Therefore, an alternative solution that can preserve MSC survival will be beneficial.…”

Section: Original Articlementioning

confidence: 99%

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Temporary storage solution for adipose derived mesenchymal stem cells

Nofianti¹,

Sari²,

Marlina³

et al. 2018

Stem Cell Investig.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

Temporary storage solution for adipose derived mesenchymal stem cells

Nofianti¹,

Sari²,

Marlina³

et al. 2018

Stem Cell Investig.

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show abstract

“…Regarding PBS, we found that cADMSCs stored in PBS for 12 h at 4 °C showed approximately 65% viability, whereas Muraki et al [51] found that the viability of hBMMSCs stored in PBS at 4 °C for 24 h was 81%. Besides, Stanislaus et al [49] also reported the viability of hUCBMSCs stored in PBS for 24 h to be > 70%.…”

Section: Discussionmentioning

confidence: 99%

“…Yan Chen et al showed that the storage of human umbilical cord blood-derived MSCs (hUCBMSCs) in normal saline for 6 h at 4 °C and RT exhibited maximum viability of 83 and 77%, respectively [48]. Stanislaus et al [49] compared the efficacy of physiologic saline, PBS, and Dulbecco's Modified Eagle Medium (DMEM)high glucose as temporary storage solutions for MSCs. They found that hUCBMSCs showed > 70% viability after storage in physiological saline for 72 h. Elisa et al [50] compared saline and DMEM as storage solutions for hADMSCs and reported that both storage solutions resulted in > 70% viability after 48 h. Their conclusion is following our result that cADMSCs stored in saline for up to 12 h showed the best viability.…”

Section: Discussionmentioning

confidence: 99%

Effects of carrier solutions on the viability and efficacy of canine adipose-derived mesenchymal stem cells

et al. 2022

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Background Mesenchymal stem cells (MSCs) have favorable characteristics that render them a potent therapeutic tool. We tested the characteristics of MSCs after temporal storage in various carrier solutions, such as 0.9% saline (saline), 5% dextrose solution (DS), heparin in saline, and Hartmann’s solution, all of which are approved by the U.S. Food and Drug Administration (FDA). Phosphate-buffered saline, which does not have FDA approval, was also used as a carrier solution. We aimed to examine the effects of these solutions on the viability and characteristics of MSCs to evaluate their suitability and efficacy for the storage of canine adipose-derived MSCs (cADMSCs). Results We stored the cADMSCs in the test carrier solutions in a time-dependent manner (1, 6, and 12 h) at 4 °C, and analyzed cell confluency, viability, proliferation, self-renewability, and chondrogenic differentiation. Cell confluency was significantly higher in 5% DS and lower in phosphate-buffered saline at 12 h compared to other solutions. cADMSCs stored in saline for 12 h showed the highest viability rate. However, at 12 h, the proliferation rate of cADMSCs was significantly higher after storage in 5% DS and significantly lower after storage in saline, compared to the other solutions. cADMSCs stored in heparin in saline showed superior chondrogenic capacities at 12 h compared to other carrier solutions. The expression levels of the stemness markers, Nanog and Sox2, as well as those of the MSC surface markers, CD90 and CD105, were also affected over time. Conclusion Our results suggest that MSCs should be stored in saline, 5% DS, heparin in saline, or Hartmann’s solution at 4 °C, all of which have FDA approval (preferable storage conditions: less than 6 h and no longer than 12 h), rather than storing them in phosphate-buffered saline to ensure high viability and efficacy.

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The Potential of Basal Medium Astemporary Prolong Culture of Umbilical Cord Derived Mesenchymalstem Cells

Pawitan¹,

Kispa²,

Liem³

et al. 2018

Biomed. Pharmacol. J

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To compare the viability and characteristics of umbilical cord mesenchymal stromal cells (UC-MSCs) when they were kept in culture in three types of αMEM after confluence for various time points. Viability and characteristic assessments were done after keeping UC-MSC confluent cultures for 0, 24, 48, 72, and 96hours in αMEM with glutamax (Himedia), and without glutamax (Himedia or Gibco).There were thirty cultures, which each ten of them were kept in the three types of basal media, and for each time point and type of basal medium, two flasks were harvested. All viability and characteristic assessments were done after harvest. Viability assessments were done in duplicate, while characteristics assessment by flowcytometry for CD73, CD90 and CD105 were done only once.Further, differences between the various time points in terms of viability and CD73, CD90 and CD105 percentage after various time points were compared and tested by appropriate statistical analysis. Viability was >70% upto 72 hours and 48 hours when the cells were kept in Himediaglutamax containing αMEM and αMEM without glutamax respectively. Flow cytometry showed that CD73, CD90 and CD negative percentage did not differ to initial percentage, but after 24 hours CD105 was slightly decreased to > 60% in Himediaglutamax containing αMEM, while in the two αMEM media without glutamaxthe percentages were below 40%. For our UC-MSC culture, glutamax containing αMEM is better compared to αMEM without glutamax as temporary storage solution, and storage should be restricted to 24 hours.

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Comparison of Various Solutions for Temporary Storage of Umbilical Cord Derived Mesenchymal Stem Cells

Cited by 4 publications

References 0 publications

Temporary storage solution for adipose derived mesenchymal stem cells

Temporary storage solution for adipose derived mesenchymal stem cells

Effects of carrier solutions on the viability and efficacy of canine adipose-derived mesenchymal stem cells

The Potential of Basal Medium Astemporary Prolong Culture of Umbilical Cord Derived Mesenchymalstem Cells

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