Comparison of Various Phenotypic Methods in Detection of Carbapenemases and Metallo-Beta-Lactamases in Carbapenem Resistant Clinical Isolates of Acinetobacter Species at a Tertiary Care Centre in North India

Goyal, Ankur; Mani, Neha K.; Chahar, Renu; Soni, A. N.; Goyal, Sapna

doi:10.20546/ijcmas.2018.701.359

Cited by 1 publication

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“…However, another study further showed results lower than we obtained in our study (39%) ( 22 ). While in contrast to our study, the study carried out in 2018 in Tehran showed a higher occurrence for ESBL production 78% ( 23 ), this result is closer to those by other study showing 88% of isolates produce ESBL ( 24 ). The variance in results between these investigations may be the result of variations in the techniques used to identify the ESBL isolates.…”

Section: Discussioncontrasting

confidence: 54%

Comparison of loop-mediated isothermal amplification, multiplex PCR, and REP- PCR techniques for identification of carbapenem-resistant Acinetobacter baumannii clinical isolates

Al jebur,

Soleimani,

Hosseini

2023

IJM

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Background and Objectives: Acinetobacter baumannii, an opportunistic pathogen, is related to hospital-acquired infections and increased mortality. This study aimed to develop the loop-mediated isothermal amplification (LAMP) test for the fast-de- tecting of A. baumannii isolates as well as determining genetic relatedness for these isolates via the REP-PCR technique. Materials and Methods: LAMP primers and multiplex PCR primers were designed for recognizing A. baumannii isolates harboring the bla SHV-1 , bla PER-1 , bla , AMPC, qnr, and aac (6)-1 genes, were collected (October 2020 to February 2021) from Shahid Motahari Hospital, Tehran, Iran. Combination disc test (CDT) results were used to assess the phenotypic iden- tification of isolates from ESBL producers. The sensitivity of the LAMP method was evaluated using a range of serial dilu- tions of genomic DNA. Results were compared between the LAMP technique, and multiplex PCR. The genetic diversity of clinical isolates was determined by REP-PCR. Results: Among one hundred A. baumannii samples and based on the combined disc test, 56% of isolates were ESBL pro- ducers. The sensitivity of the LAMP technique for the identification of A. baumannii was 4.06 ng/μl whilst the multiplex PCR was (16.2 ng/μl). Regarding multiplex PCR, (68%) of the isolates were bla SHV-1 positive, (40%) bla , (85%) aac (6́)-1, AMPC (67%), bla TEM-1 (63%), and (15%) qnr respectively. While in LAMP, (69%) of isolates were bla positive, (86%) aac (6')-1, and (20%) qnr. The results of AMPC, bla TEM-1 , and bla PER-1 genes showed 100% compatibility between multiplex PCR and LAMP assays. The results of REP-PCR indicated there were 17 clones, clone A at 14% was the most prevalent of the isolates. Conclusion: Wherever equipment and financial constraints are crucial, the LAMP test offers a better and more potent detec- tion rate for the identification of A. baumannii isolates than multiplex PCR. Furthermore, the genetic diversity of A. bauman- nii in these clinical isolates showed frequent commonality of genotypes.

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Section: Discussioncontrasting

confidence: 54%