2001
DOI: 10.1161/hc4401.098490
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Comparison of Various Electrophoretic Characteristics of LDL Particles and Their Relationship to the Risk of Ischemic Heart Disease

Abstract: Background-Several cross-sectional studies and 3 prospective, nested, case-control studies have indicated that individuals with small, dense low density lipoprotein (LDL) particles are at increased risk for ischemic heart disease (IHD). However, whether LDL particle size is an independent risk factor for future IHD events remains controversial. The objective of the present study was to further analyze the cardiovascular risk associated with various electrophoretic characteristics of LDL particles in men. Metho… Show more

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Cited by 236 publications
(168 citation statements)
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“…Intra-and interassay coefficients of variation for this measurement were both p6.0%, as described previously (Couillard et al, 2003). Distinct subpopulations of LDL particles in whole plasma were separated by size using a nondenaturing 2-16% gradient gel electrophoresis, as described previously (St Pierre et al, 2001;Archer et al, 2003b). Postheparin (60 IU/kg body weight) lipoprotein lipase (LPL) and hepatic lipase (HL) activities were measured after preincubation with sodium dodecyl sulfate (SDS), as previously described (Watson et al, 1995;Bower et al, 2002).…”
Section: Laboratory Methodsmentioning
confidence: 99%
“…Intra-and interassay coefficients of variation for this measurement were both p6.0%, as described previously (Couillard et al, 2003). Distinct subpopulations of LDL particles in whole plasma were separated by size using a nondenaturing 2-16% gradient gel electrophoresis, as described previously (St Pierre et al, 2001;Archer et al, 2003b). Postheparin (60 IU/kg body weight) lipoprotein lipase (LPL) and hepatic lipase (HL) activities were measured after preincubation with sodium dodecyl sulfate (SDS), as previously described (Watson et al, 1995;Bower et al, 2002).…”
Section: Laboratory Methodsmentioning
confidence: 99%
“…Both LDL size and the number of particles seems to increase synergistically the risk of CHD [120,121]. Recently convincing tenets seem to explain the atherogeneity of small dense LDL particles.…”
Section: Why Is Small Dense Ldl Highly Atherogenic?mentioning
confidence: 99%
“…19 Distinct subpopulations of LDL particles were separated in whole plasma using nondenaturating 2-16% gradient gel electrophoresis as previously described. 1 Lowdensity lipoprotein peak particle diameter was identified as the most important subclass of LDL in each individual and was calculated from calibration curves using plasma standards of known diameter. The CV of the calculated particle diameters was estimated to be o1%.…”
Section: Laboratory Methodsmentioning
confidence: 99%
“…A similar approach was used to assess the relative proportion of LDL particles with a diameter 4260 Å (LDL% 4260 Å ) and absolute concentrations of cholesterol in particles with a diameter 4260 Å (LDL-C 4260 Å ). 1 Oxidized LDL (OxLDL) concentrations were measured using a commercial ELISA according to the manufacturer's instructions (Alpco Diagnostics, Windham, NH). C-reactive protein levels were measured with a highly sensitive commercial immunoassay (Dade Behring, Mississauga, ON, Canada) as described previously.…”
Section: Laboratory Methodsmentioning
confidence: 99%
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