Comparison of University of Wisconsin and ET-Kyoto Preservation Solutions for the Cryopreservation of Primary Human Hepatocytes

Illouz, Séverine; Nakamura, Tõru; Webb, M’Balu A.; Thava, B.; Bikchandani, J.; Robertson, G. S. M.; Lloyd, D. M.; Berry, David P.; Wada, Hiromi; Dennison, Ashley R.

doi:10.1016/j.transproceed.2008.01.064

Cited by 8 publications

(3 citation statements)

References 18 publications

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“…Conditions for cryopreservation of isolated hepatocytes have been explored extensively, especially for the purpose of preserving human hepatocytes − ,− . Further optimization of those conditions is an ongoing area that attracts active research , .…”

Section: Resultsmentioning

confidence: 99%

Cryopreserved Hepatocytes from Rainbow Trout (Oncorhynchus mykiss): A Validation Study to Support Their Application in Bioaccumulation Assessment

Mingoia

Glover

Nabb

et al. 2010

Environ. Sci. Technol.

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Determination of biotransformation rates of xenobiotics in freshly isolated trout hepatocytes has been demonstrated to significantly improve the performance of bioaccumulation assessment models. In order to promote this in vitro approach, trout hepatocytes need to be cryopreserved to facilitate their availability while ensuring their metabolic competency. In the present study, we obtained basal level metabolic enzyme activities for cytochrome P450 (CYP) 1A, CYP3A, glutathione-S-transferase, and uridine 5'-diphospho-glucuronosyltransferase from trout hepatocytes cryopreserved for various periods of time up to three months and compared their values with those obtained from freshly isolated hepatocytes. Similarly, we compared intrinsic clearance (CL(int)) values determined in cryopreserved trout hepatocytes to those determined in freshly isolated hepatocytes for reference compounds molinate, michler's ketone, 4-nonylphenol, 2,4-ditert-butylphenol, benzo(a)pyrene, and pyrene. Our results show that cryopreserved trout hepatocytes maintained greater than 75% of their basal level enzyme activities and greater than 72% of xenobiotic biotransformation capabilities, regardless of the length of cryostorage. As a result, bioconcentration factors of the reference compounds were adequately predicted based on the CL(int) values. We simulated the condition for shipping cryopreserved trout hepatocytes and demonstrated that 24 h dry ice storage did not negatively affect the rates of xenobiotic biotransformation. We conclude that cryopreserved trout hepatocytes are suitable for biotransformation rate determination of xenobiotics in vitro, and therefore, are an acceptable alternative to freshly isolated trout hepatocytes in the application in bioaccumulation assessment.

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Section: Resultsmentioning

confidence: 99%

Cryopreserved Hepatocytes from Rainbow Trout (Oncorhynchus mykiss): A Validation Study to Support Their Application in Bioaccumulation Assessment

Mingoia

Glover

Nabb

et al. 2010

Environ. Sci. Technol.

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“…Static cold storage is the most prevalent method for preservation of renal grafts for transplantation (14,17). The protective effects of various preservation solutions have been studied extensively in both clinical trials (11,15) and several experimental animal models (5,12,21). However, few studies compared the preservation solutions in mouse KTX.…”

Section: Introductionmentioning

confidence: 99%

Effects of different storage solutions on renal ischemia tolerance after kidney transplantation in mice

Wang

Jin

Jiang

et al. 2018

American Journal of Physiology-Renal Physiology

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storage is the most prevalent method for graft preservation in kidney transplantation (KTX). The protective effects of various preservation solutions have been studied extensively in both clinical trials and experimental animal models. However, a paucity of studies have examined the effect of different preservation solutions on graft function in mouse KTX; in addition, the tolerance of the transplanted grafts to further insult has not been evaluated, which was the objective of the present study. We performed mouse KTX in three groups, with the donor kidneys preserved in different solutions for 60 min: saline, mouse serum, and University of Wisconsin (UW) solution. The graft functions were assessed by kidney injury markers and glomerular filtration rate (GFR). The grafts that were preserved in UW solution exhibited better functions, reflected by 50 and 70% lower plasma creatinine levels as well as 30 and 55% higher plasma creatinine levels in GFR than serum and saline groups, respectively, during the first week after transplants. To examine the graft function in response to additional insult, we induced ischemia-reperfusion injury (IRI) by clamping the renal pedicle for 18 min at 4 wk after KTX. We found that the grafts preserved in UW solution exhibited ~30 and 20% less injury assessed by kidney injury markers and histology than in other two preservation solutions. Taken together, our results demonstrated that UW solution exhibited a better protective effect in transplanted renal grafts in mice. UW solution is recommended for use in mouse KTX for reducing confounding factors such as IRI during surgery.

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“…Therefore, we have been trying to improve cell survival by a modification of the freezing medium. Previously, we reported the different efficacies of oligo-saccharides on the attachment capability of cryopreserved hepatocytes and arrived at the conclusion that maltose is the most effective additive (6), while trehalose, which belongs to the same dissacharide as maltose, is thought to be effective elsewhere (3, 4). Therefore, we embarked on the development of a serum-free freezing solution to ensure the safety of cells for clinical transplantation, especially to avoid xenozoonoses.…”

Section: Introductionmentioning

confidence: 99%

An Improvement in the Attaching Capability of Cryopreserved Human Hepatocytes by a Proteinaceous High Molecule, Sericin, in the Serum-Free Solution

et al. 2010

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The methodology of cryopreservation of human hepatocytes remains unsatisfactory. Even when the viability of thawed cells is tolerable, the cells often lose the attaching capability to a culture dish, resulting in the cells' inability to survive. Previously, we described the effectiveness of maltose on the attachment of hepatocytes. This article demonstrates that a silk-derived high molecular protein, sericin, improves the cell-attaching capability in the serum-free freezing medium. When human hepatocytes [initial viability: 60.9 ± 3.1% (mean ± SD, n = 3)] were frozen with serum-free Dulbecco's modified Eagle medium (DMEM) containing 10% dimethyl sulfoxide (DMSO), the viability was 29.4 ± 3.2% and the cell-attaching capability 20.4 ± 4.1%. On the other hand, DMEM containing 10% DMSO and 1% sericin increased the values to 45.0 ± 0.8% and 26.2 ± 3.2%. Moreover, the addition of 0.1 mol/L maltose to the sericin-containing medium improved to 42.2 ± 3.2% and 51.1 ± 1.0%, as we demonstrated in a previous report. The present results indicated that sericin combined with maltose is a novel additive in the serum-free freezing medium for human hepatocytes.

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Comparison of University of Wisconsin and ET-Kyoto Preservation Solutions for the Cryopreservation of Primary Human Hepatocytes

Cited by 8 publications

References 18 publications

Cryopreserved Hepatocytes from Rainbow Trout (Oncorhynchus mykiss): A Validation Study to Support Their Application in Bioaccumulation Assessment

Cryopreserved Hepatocytes from Rainbow Trout (Oncorhynchus mykiss): A Validation Study to Support Their Application in Bioaccumulation Assessment

Effects of different storage solutions on renal ischemia tolerance after kidney transplantation in mice

An Improvement in the Attaching Capability of Cryopreserved Human Hepatocytes by a Proteinaceous High Molecule, Sericin, in the Serum-Free Solution

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