Comparison of ultrastructural changes and the anticarcinogenic effects of thymol and carvacrol on ovarian cancer cells: which is more effective?

Elbe, Hülya; Yiğittürk, Gürkan; Çavuşoğlu, Türker; Baygar, Tuba; Onal, Melike Ozgul; Öztürk, Feral

doi:10.1080/01913123.2020.1740366

Cited by 36 publications

(25 citation statements)

References 42 publications

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“…Moreover, Hazem Elshafie et al in 2017 analyzed the in vitro cytotoxic potential of the compounds present in a higher proportion in oregano against hepatocarcinoma cells (HepG2), reporting that carvacrol was the compound with the greatest potential to inhibit the proliferation of this cell line, the lethal dose 50 found in this study was 48 mg/L [22]. These results, among some other studies that have been carried out on this compound and its properties against cancer cells are consistent with the fact that carvacrol has a potent in vitro cytotoxic effect against different cancer cell lines like MDA-231 and MCF-7 [31][32][33][34][35][36][37]. However, the present study provides the first evidence on the effectiveness of this compound against TN BC cells (HCC-70), demonstrating the potency of its cytotoxic activity against aggressive subtypes of BC.…”

Section: Cytotoxicitysupporting

confidence: 89%

Dose-Dependent Cytotoxicity of the Origanum vulgare and Carvacrol on Triple Negative Breast Cancer Cell Line

Rojo-Ruvalcaba

Cobián

Pascoe-González

et al. 2020

First International Electronic Conference on Nutrients, Microbiota and Chronic Disease

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Uncontrolled proliferation and death resistance are two hallmarks of cancer cells. Breast cancer (BC) has the highest incidence and mortality in women worldwide and national; subtype triple negative (TN) is the most aggressive type because it is not susceptible to conventional therapy. Since 2004, the World Health Organization (WHO) approved use of alternative treatments as adjuvants based on evidence of its benefits. Since then, different natural alternatives to treat cancer have been studied, including Origanum vulgare (Ov) and carvacrol (Crv), one of its main compounds. However, cytotoxic potential of these products has not been studied in this subtype of BC. The objective of this study was to evaluate the cytotoxic effect of Ov and Crv on TN BC cell line (HCC-70). Lethal dose 50 was determined on control cell line HaCaT through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays with Ov and Crv stimulus in different doses and concentrations; the found dose was used on the HCC-70 cell line. The results were evaluated by ANOVA, finding that Ov reduced the proliferation by 94.05% ± 0.11 (p < 0.001) and Crv by 93.43% ± 0.21 (p < 0.001) compared to untreated cells. This suggests that both Ov and Crv present a powerful cytotoxic effect against the HCC-70 cell line and are promising options that should receive further study.

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Section: Cytotoxicitysupporting

confidence: 89%

Dose-Dependent Cytotoxicity of the Origanum vulgare and Carvacrol on Triple Negative Breast Cancer Cell Line

Rojo-Ruvalcaba

Cobián

Pascoe-González

et al. 2020

First International Electronic Conference on Nutrients, Microbiota and Chronic Disease

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“…In SiHa cells, the reduction in viability and apoptosis induction occurred through p53 activation and Bax, caspase-3, -6, -9 expression, along, with negative regulation of Bcl-2 gene ( Abbas and Al-Fatlawi, 2018 ). Furthermore, another study demonstrated that carvacrol and thymol were cytotoxic against ovarian cancer (SKOV-3 cell line) exhibiting apoptotic and antiproliferative properties ( Elbe et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

“…Treatment with carvacrol in leiomyosarcoma cells exhibited antiproliferative effects in a concentration dependent manner and also inhibition of cell growth (Karkabounas et al, 2006). In addition, carvacrol showed a greater cytotoxicity compared to thymol against murine mast cell cells (P-815 cell line) (Jaafari et al, 2007;Jaafari et al, 2012), with accumulation of cells in the S phase (Jaafari et al, 2012).…”

Section: Sarcomasmentioning

confidence: 97%

“…Anticarcinogenic effects were observed after treatment with carvacrol in Wistar rats, depicted by a reduction in the incidence of tumors, increased survival rate, and a reduced carcinogenic potency of the substance in inducing malignant tumors (Karkabounas et al, 2006). The pre-and post-treatment with carvacrol in animals with liver cancer induced by diethylnitrosamine (DEN) revealed a decrease in the number of nodules, a final body weight increase and a reduction in liver weight.…”

Section: Description Of In Vivo Studies With Carvacrol and Thymolmentioning

confidence: 99%

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Antitumor Effects of Carvacrol and Thymol: A Systematic Review

et al. 2021

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Background: It is estimated that one in five people worldwide faces a diagnosis of a malignant neoplasm during their lifetime. Carvacrol and its isomer, thymol, are natural compounds that act against several diseases, including cancer. Thus, this systematic review aimed to examine and synthesize the knowledge on the antitumor effects of carvacrol and thymol.Methods: A systematic literature search was carried out in the PubMed, Web of Science, Scopus and Lilacs databases in April 2020 (updated in March 2021) based on the PRISMA 2020 guidelines. The following combination of health descriptors, MeSH terms and their synonyms were used: carvacrol, thymol, antitumor, antineoplastic, anticancer, cytotoxicity, apoptosis, cell proliferation, in vitro and in vivo. To assess the risk of bias in in vivo studies, the SYRCLE Risk of Bias tool was used, and for in vitro studies, a modified version was used.Results: A total of 1,170 records were identified, with 77 meeting the established criteria. The studies were published between 2003 and 2021, with 69 being in vitro and 10 in vivo. Forty-three used carvacrol, 19 thymol, and 15 studies tested both monoterpenes. It was attested that carvacrol and thymol induced apoptosis, cytotoxicity, cell cycle arrest, antimetastatic activity, and also displayed different antiproliferative effects and inhibition of signaling pathways (MAPKs and PI3K/AKT/mTOR).Conclusions: Carvacrol and thymol exhibited antitumor and antiproliferative activity through several signaling pathways. In vitro, carvacrol appears to be more potent than thymol. However, further in vivo studies with robust methodology are required to define a standard and safe dose, determine their toxic or side effects, and clarify its exact mechanisms of action.This systematic review was registered in the PROSPERO database (CRD42020176736) and the protocol is available at https://www.crd.york.ac.uk/prospero/display_record.php?RecordID=176736.

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“…Apoptosis acceptance was identified by acridine orange/ethidium bromide recoloring and western smudging underneath the halfmaximal development inhibitory fixation esteem. Carvacrol demonstrated cytotoxic, genotoxic, apoptotic, ROS producing, and GSH-diminishing impacts on AGS cells in a portion subordinate way [11][12][13]. There was a nearby negative connection between cell practicality and ROS level.…”

Section: Introductionmentioning

confidence: 99%

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et al. 2021

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A big risk factor for sensitization and allergic diseases is susceptibility to animal allergens. The most significant animal allergens are derived from mammals, besides mites and cockroaches. The experiments on the allergenic properties of KUD975 were carried out on bisexual sexually mature albino guinea pigs weighing 300 ± 20 g. During the initial assessment of allergenic and mutagenic properties, the allergenic properties of KUD975 were studied by setting up an active skin anaphylaxis reaction. Mutagenicity in the test of induction of dominant lethal mutations was studied. For research, outbred male rats weighing 180-220 g KUD975 was administered intragastrically at a dose of 106 mg/kg, which corresponds to a 40-fold estimated therapeutic dose. Planting intact females at the rate of 3 heads for each male was performed weekly according to the stages of spermatogenesis. The study of the allergenic effect in the formulation of the reaction of active skin anaphylaxis in guinea pigs and mutagenicity in the test for the induction of dominant lethal mutations of the phenolic compound methyl ester (2 -((1hydroxy naphthalene-2-yl) thio) acetyl) -D-proline under the laboratory code KUD975 showed no signs of allergenicity and mutagenicity of compound KUD975. Given the results of this study, as well as the previously conducted study of toxicological safety, pharmacological activity, methyl ester (2 -((1hydroxy naphthalene-2-yl) thio) acetyl) -D-proline KUD-975 can be recommended for further study as a medicinal agent acting on arignase-2 for the treatment and prevention of a number of endothelium-associated diseases.

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Comparison of ultrastructural changes and the anticarcinogenic effects of thymol and carvacrol on ovarian cancer cells: which is more effective?

Cited by 36 publications

References 42 publications

Dose-Dependent Cytotoxicity of the Origanum vulgare and Carvacrol on Triple Negative Breast Cancer Cell Line

Dose-Dependent Cytotoxicity of the Origanum vulgare and Carvacrol on Triple Negative Breast Cancer Cell Line

Antitumor Effects of Carvacrol and Thymol: A Systematic Review

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