Comparison of ultrasound and electromagnetic field effects on osteoblast growth

Li, Jimmy Kuan-Jung; Lin, Cheng-An J.; Liu, Hwa‐Chang; Sun, Jui Sheng; Ruaan, Ruoh‐Chyu; Shih, Chun Kuang; Chang, Walter Hong‐Shong

doi:10.1016/j.ultrasmedbio.2006.01.017

Cited by 58 publications

(13 citation statements)

References 23 publications

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“…Considering that some experimental studies have observed the effect generated by external biophysical stimuli over the cell membrane receptors, it is noteworthy to mention that this study presents certain limitations in order to validate the computational results obtained. For this reason, an experimental validation is necessary to observe how different frequencies modify the cell membrane response in terms of ion concentration flows and their incidence on the cell dynamics, such as viability, migration, proliferation, and molecular synthesis …”

Section: Discussionmentioning

confidence: 99%

Effect of magnetic and electric fields on plasma membrane of single cells: A computational approach

Escobar

Vaca‐González

Guevara

et al. 2020

Engineering Reports

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Cell membrane is a lipid bilayer that allows the flow of ions through their ionic pumping proteins. The ionic flow can be stimulated with external stimuli to activate specific signaling pathways intracellularly. Although studies have applied electric and magnetic stimuli to modify the cell function, the parameters to stimulate the cell membrane are unknown. Accordingly, a computational model to simulate the effect of electric and magnetic fields on the cell membrane was developed. Cells were stimulated with electric fields from 45 × 103 V/m to 12.6 × 105 V/m and magnetic fields of 2 mT, at frequencies of 60 kHz, 10 MHz, and 1 GHz. Results showed that the electric fields applied to the cell membrane tend to increase according to the frequency used, while magnetic fields do not have any effect on it. It was observed that electric fields generate a high voltage concentrator in the cell membrane of ellipsoidal cells when a frequency window from 1 kHz to 1 GHz was applied. These findings demonstrate that depending on the intensity of the field and frequency, it was possible to stimulate different cell membrane zones. This model is a promising tool to establish the adequate parameters to stimulate cells, and accurately predict if the stimulation modifies the cell membrane potential.

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Section: Discussionmentioning

confidence: 99%

Effect of magnetic and electric fields on plasma membrane of single cells: A computational approach

Escobar

Vaca‐González

Guevara

et al. 2020

Engineering Reports

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“…These results are consistent with that observed by Tong et al [] using a different experimental model in murine MC3T3 cells, where exposure to 5 mT, 15 Hz PRF PEMF bursts of 4.5 kHz rectangular pulses increased intracellular calcium transients in the presence of high extracellular Ca 2+ concentrations. Similarly, it has been shown that exposure to even lower frequencies (0.3 ms impulses at 7.5 Hz signaling, inducing a 2 mV/cm electric field) for 2 h stimulated rat calvaria cell proliferation via a Ca 2+ ‐ and Calmodulin‐mediated pathway [Kuan‐Jung Li et al, ]. Recently, it has been shown that exposure to 75 Hz, 2 mT PEMFs with 1.3 ms pulses (induced electric field 5 mV) for 10 min a day, as previously determined [De Mattei et al, ; Fassina et al, ], promoted the osteogenic differentiation of hBMMSCs cultured in osteogenic medium [Petecchia et al, ].…”

Section: Effects Of Pemfs On Osteoblastsmentioning

confidence: 65%

“…They also observed an increased production of Prostaglandin E 2 (PGE 2 ), a soluble factor that has been shown to affect osteoblast differentiation, with a stimulation of just 20 min/day, after 48 h [Li et al, ]. Interestingly, a single 2 h‐long exposure of the same PEMF regime was shown to significantly increase rat calvaria cell viability by MTT after 1 day [Kuan‐Jung Li et al, ], indicating that primary cells may be a better model to detect changes in cell proliferation with low levels of stimulation. Consistently with this idea, a 2 h‐long exposure to 7.5 Hz PEMFs was also able to affect the proliferation and differentiation of human bone marrow mesenchymal stem cells (hBMMSC) by day 5, although its effects were quite complex and will be reviewed later below [Tsai et al, ].…”

Section: Effects Of Pemfs On Osteoblastsmentioning

confidence: 99%

The cellular effects of Pulsed Electromagnetic Fields on osteoblasts: A review

Galli

Pedrazzi

Guizzardi

2019

Bioelectromagnetics

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Electromagnetic fields (EMFs) have long been known to interact with living organisms and their cells and to bear the potential for therapeutic use. Among the most extensively investigated applications, the use of Pulsed EMFs (PEMFs) has proven effective to ameliorate bone healing in several studies, although the evidence is still inconclusive. This is due in part to our still‐poor understanding of the mechanisms by which PEMFs act on cells and affect their functions and to an ongoing lack of consensus on the most effective parameters for specific clinical applications. The present review has compared in vitro studies on PEMFs on different osteoblast models, which elucidate potential mechanisms of action for PEMFs, up to the most recent insights into the role of primary cilia, and highlight the critical issues underlying at least some of the inconsistent results in the available literature. Bioelectromagnetics. 2019;9999:XX–XX. © 2019 Bioelectromagnetics Society.

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“…Moreover, the activated Ca 2+ /nitric oxide/cGMP cascade is also closely related to the increased expression of FGF-2 and VEGF, two key regulators of angiogenesis [27]. In addition, the crosstalk between Ca 2+ , ERK, PKA, and PKG signaling under PEMF stimulation was also reported [19, 22]. All these findings show the prominent role of Ca 2+ signaling in PEMFs-induced bone repair.…”

Section: Underlying Signaling Pathwaysmentioning

confidence: 99%

“…Intracellular Ca 2+ is generally considered as one of the main actors to translate the PEMF signal into a biological signal [18]. Many studies revealed that PEMF signal passes through the cell membrane to set up a time-varying electrical field within the cytosol; this electrical field subsequently induces the release of intracellular Ca 2+ , leading to increases in cytosolic calcium and activated calmodulin and the enhancement of bone cell viability [17, 19, 20]. Voltage-gated Ca channels (VGCCs), especially the L type, play a pivotal role in intracellular Ca 2+ release.…”

Section: Underlying Signaling Pathwaysmentioning

confidence: 99%

Underlying Signaling Pathways and Therapeutic Applications of Pulsed Electromagnetic Fields in Bone Repair

Yuan

Xin

Jiang

2018

Cell Physiol Biochem

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Pulsed electromagnetic field (PEMF) stimulation, as a prospective, noninvasive, and safe physical therapy strategy to accelerate bone repair has received tremendous attention in recent decades. Physical PEMF stimulation initiates the signaling cascades, which effectively promote osteogenesis and angiogenesis in an orchestrated spatiotemporal manner and ultimately enhance the self-repair capability of bone tissues. Considerable research progresses have been made in exploring the underlying cellular and subcellular mechanisms of PEMF promotion effect in bone repair. Moreover, the promotion effect has shown strikingly positive benefits in the treatment of various skeletal diseases. However, many preclinical and clinical efficacy evaluation studies are still needed to make PEMFs more effective and extensive in clinical application. In this review, we briefly introduce the basic knowledge of PEMFs on bone repair, systematically elaborate several key signaling pathways involved in PEMFs-induced bone repair, and then discuss the therapeutic applications of PEMFs alone or in combination with other available therapies in bone repair, and evaluate the treatment effect by analyzing and summarizing recent literature.

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Comparison of ultrasound and electromagnetic field effects on osteoblast growth

Cited by 58 publications

References 23 publications

Effect of magnetic and electric fields on plasma membrane of single cells: A computational approach

Effect of magnetic and electric fields on plasma membrane of single cells: A computational approach

The cellular effects of Pulsed Electromagnetic Fields on osteoblasts: A review

Underlying Signaling Pathways and Therapeutic Applications of Pulsed Electromagnetic Fields in Bone Repair

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