Comparison of two ultracentrifugation procedures for separation of nonhuman primate lipoproteins

Hoinacki, J.; Nicolosi, Robert J.; Hoover, G A; Llansa, Norma; Ershow, Abby G.; Lozy, Mohamed el; Hayes, K. C.

doi:10.1016/0003-2697(78)90448-7

Cited by 8 publications

(2 citation statements)

References 23 publications

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“…Isolation and Acetylation of LDL LDL was isolated from fresh human serum by sequential gradient ultracentrifugation [45]. Afterwards LDL was dialyzed for 24 h at 4°C against a buffer containing 0.15 mol/L NaCl and 0.3 mmol/L EDTA, pH 7.4.…”

Section: Fatty Acid Analysis Of Cell Total Lipidsmentioning

confidence: 99%

Conjugated Linoleic Acid Isomers Reduce Cholesterol Accumulation in Acetylated LDL‐Induced Mouse RAW264.7 Macrophage‐Derived Foam Cells

Ringseis

Wen

Saal

et al. 2008

Lipids

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Synthetic activators of peroxisome proliferator-activated receptors (PPAR)-alpha and -gamma are capable of reducing macrophage foam cell cholesterol accumulation through the activation of genes involved in cholesterol homeostasis. Since conjugated linoleic acids (CLA) were also demonstrated to activate PPARalpha and PPARgamma in vivo and in vitro, we tested the hypothesis that CLA are also capable of reducing macrophage foam cell cholesterol accumulation. Thus, mouse RAW264.7 macrophage-derived foam cells were treated with CLA isomers, c9t11-CLA and t10c12-CLA, and linoleic acid (LA), as reference fatty acid, and analyzed for the concentrations of free and esterified cholesterol, cholesterol efflux and expression of genes involved in cholesterol homeostasis (CD36, ABCA1, LXRalpha, NPC-1, and NPC-2). Treatment with c9t11-CLA and t10c12-CLA, but not LA, lowered cholesterol accumulation, stimulated acceptor-dependent cholesterol efflux, and increased relative mRNA concentrations of CD36, ABCA1, LXRalpha, NPC-1, and NPC-2 (P < 0.05). In conclusion, the present study showed that CLA isomers reduce cholesterol accumulation in RAW264.7 macrophage-derived foam cells presumably by enhancing lipid acceptor-dependent cholesterol efflux.

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Section: Fatty Acid Analysis Of Cell Total Lipidsmentioning

confidence: 99%

Conjugated Linoleic Acid Isomers Reduce Cholesterol Accumulation in Acetylated LDL‐Induced Mouse RAW264.7 Macrophage‐Derived Foam Cells

Ringseis

Wen

Saal

et al. 2008

Lipids

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“…Density gradient ultracentrifugation has long been the gold standard for separation, identification, and quantification of lipoproteins. − Ultracentrifugal methods separate lipoproteins based on their hydrated densities. The different forms of this technique include rate zonal ultracentrifugation and isopycnic separations. , Each of these techniques has specific advantages and disadvantages including the accuracy of the separation, use in fraction preparation, and the extent of skill needed to perform these techniques.…”

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confidence: 99%

Developing High Performance Lipoprotein Density Profiling for Use in Clinical Studies Relating to Cardiovascular Disease

et al. 2011

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Early detection of the beginning stage of cardiovascular disease (CVD) is an approach to prevention because the process is reversible at this stage. Consequently, several methods for screening for CVD have been introduced in recent years incorporating different analytical methods for characterizing the population of blood-borne lipoprotein subclasses. The gold standard method for lipoprotein subclassification is based on lipoprotein density measured by sedimentation equilibrium using the ultracentrifuge. However, this method has not been adopted for clinical studies because of difficulties in achieving the precision required for distinguishing individuals with and without CVD particularly when statistical classification methods are used. The objective of this study was to identify and improve the major factors that influence the precision of measurement of lipoprotein density profile by sedimentation equilibrium analysis and labeling with a fluorescent probe. The study has two phases, each contributing to precision. The first phase focuses on the ultracentrifugation-related variables, and the second phase addresses those factors involved in converting the fluorescent lipoprotein density profile to a digital format compatible with statistical analysis. The overall improvement in precision was on the order of a factor of 5, sufficient to be effectively applied to ongoing classification studies relating to CVD risk assessment.

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Effect of cigarette smoking on high density lipoprotein phospholipids

Hegarty

Turgiss

Mulligan

et al. 1982

Biochemical and Biophysical Research Communications

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Comparison of two ultracentrifugation procedures for separation of nonhuman primate lipoproteins

Cited by 8 publications

References 23 publications

Conjugated Linoleic Acid Isomers Reduce Cholesterol Accumulation in Acetylated LDL‐Induced Mouse RAW264.7 Macrophage‐Derived Foam Cells

Conjugated Linoleic Acid Isomers Reduce Cholesterol Accumulation in Acetylated LDL‐Induced Mouse RAW264.7 Macrophage‐Derived Foam Cells

Developing High Performance Lipoprotein Density Profiling for Use in Clinical Studies Relating to Cardiovascular Disease

Effect of cigarette smoking on high density lipoprotein phospholipids

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