Comparison of two types of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for the identification and typing of Clostridium difficile

Kiyosuke, Makiko; Kibe, Yasushi; Oho, Megumi; Kusaba, Koji; Shimono, Nobuyuki; Hotta, Taeko; Kang, Dongchon; Shoubuike, Takeo; Miyamoto, Hiroshi

doi:10.1099/jmm.0.000136

Cited by 10 publications

(3 citation statements)

References 21 publications

(17 reference statements)

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“…Rapid detection methods evaluated and reported in Japan include detection of GDH and toxin A/B in feces (using Immunocard ® and TOX A/B, respectively) [ 47 ]; and detection of GDH and toxin A/B simultaneously using C DIFF QUIK CHEK COMPLETE ® [ 41 ]. Although mass spectrometry methods have also been reported, these were not considered suitable for typing C. difficile [ 48 ].…”

Section: Resultsmentioning

confidence: 99%

The Epidemiology of Clostridium difficile Infection in Japan: A Systematic Review

Riley

Kimura

2018

Infect Dis Ther

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To increase understanding of the epidemiology, risks, consequences and resource utilization of Clostridium difficile infection (CDI) in Japan, a systematic literature review was undertaken of relevant publications from January 2006 to November 2017. Using the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and methods, 55 articles met the criteria for full review. The majority (58%) of studies were from a single site, with the most recent data from 2015. The incidence, reported prevalence and recurrence rate of CDI in Japan were 0.8–4.71/10,000 patient-days, 0.3–5.5/1000 patients and 3.3–27.3%, respectively, and varied according to setting, population, CDI definition and detection method. Most C. difficile isolates associated with CDI in Japan were toxin A+B+, with a low level of C. difficile binary toxin-positive (CDT+) strains (0–6.8% reported across studies). The most common C. difficile PCR ribotypes associated with infection in Japan were smz/018, 002, 052 and 369. Data regarding the impact of CDI on length of hospital stay were limited. Reported all-cause mortality in patients with CDI ranged from 3.4 to 15.1% between 2007 and 2013. Two studies assessed risk factors for CDI recurrence, identifying malignant disease, intensive care unit hospitalization and use of proton pump inhibitors as factors increasing the risk of initial and/or recurrent CDI. No study analyzed initial CDI treatment in relation to recurrence. More comprehensive surveillance and coordinated studies are needed to map trends, understand risk factors, and recognize the extent and impact of CDI in Japanese patients.FundingAstellas Pharma, Inc.Plain Language SummaryPlain language summary available for this article.Electronic supplementary materialThe online version of this article (10.1007/s40121-018-0186-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

The Epidemiology of Clostridium difficile Infection in Japan: A Systematic Review

Riley

Kimura

2018

Infect Dis Ther

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“…Other important questions include the diagnostic performance with local spectrum of microorganisms and finally, the differences between the two available commercial systems [48]. Direct comparative studies of both systems are scarce and mostly derive from industrialized countries in Europe, Asia, and North America [16,37,49–52]. Therefore, there is a need for evaluations in non-industrialized regions using local microbial samples and routine laboratory infrastructure.…”

Section: Discussionmentioning

confidence: 99%

Head-to-head comparison of Microflex LT and Vitek MS systems for routine identification of microorganisms by MALDI-TOF mass spectrometry in Chile

et al. 2017

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BackgroundMatrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is a new and revolutionary identification method for microorganisms and has recently been introduced into clinical microbiology in many industrialized countries in Europe and North America.ObjectivesOur study aimed to compare the performance and practicality of two commercial MALDI-TOF MS platforms in a head-to head manner at a routine laboratory in Chile.MethodsDuring a five-month period in 2012–13, the diagnostic efficiency (correct identification rate) and agreement between Microflex LT (Bruker Daltonics) and Vitek MS (bioMérieux) was compared in a parallel manner to conventional identification including genotypic analysis for difficult-to-identify strains. The study included 804 microbial isolates: 252 Enterobacteriaceae, 126 non-fermenters, 36 other gram-negative rods, 279 gram-positive cocci, 32 gram-positive rods, 32 anaerobes, and 47 yeasts. Other relevant factors of the two devices such as user friendliness and connectivity were also evaluated and compared.ResultsBoth systems correctly identified the vast majority (98%) of the isolates to the genus level. Vitek MS reached higher rates of identification to species and species complex level than Microflex LT (81% vs. 85% and 87% vs. 93%, respectively), which was mainly based on the higher performance among coagulase negative staphylococci and Candida isolates. The evaluation of user friendliness and other technical aspects showed only marginal differences, which slightly favored Vitek MS, mainly due to its ready-to-use supplies, easier connectivity and workflow integration, and availability of local technical support.ConclusionsBoth MALDI-TOF MS systems permitted fast and accurate identification of most microbial strains and showed a high level of user-friendliness. The observed differences were marginal and slightly favored Vitek MS, mainly due to practicality and connectivity issues within our setting.

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“…C. difficile colonies show typical grey to black, irregular, and rough colonies. We selected suspicious colonies by plating on chromogenic medium and used matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS French Bio-Merieux) for identification [ 48 ]. The identification result had to be C. difficile with a confidence level of 99.9% and a green box mark.…”

Section: Methodsmentioning

confidence: 99%

Toxin genotypes, antibiotic resistance and their correlations in Clostridioides difficile isolated from hospitals in Xi’an, China

Zhang,

Ma,

Zhang

et al. 2024

BMC Microbiol

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Background Clostridioides difficile is the main pathogen of antimicrobial-associated diarrhoea and health care facility-associated infectious diarrhoea. This study aimed to investigate the prevalence, toxin genotypes, and antibiotic resistance of C. difficile among hospitalized patients in Xi’an, China. Results We isolated and cultured 156 strains of C. difficile, representing 12.67% of the 1231 inpatient stool samples collected. Among the isolates, tcdA + B + strains were predominant, accounting for 78.2% (122/156), followed by 27 tcdA-B + strains (27/156, 17.3%) and 6 binary toxin gene-positive strains. The positive rates of three regulatory genes, tcdC, tcdR, and tcdE, were 89.1% (139/156), 96.8% (151/156), and 100%, respectively. All isolates were sensitive to metronidazole, and the resistance rates to clindamycin and cephalosporins were also high. Six strains were found to be resistant to vancomycin. Conclusion Currently, the prevalence rate of C. difficile infection (CDI) in Xi’an is 12.67% (156/1231), with the major toxin genotype of the isolates being tcdA + tcdB + cdtA-/B-. Metronidazole and vancomycin were still effective drugs for the treatment of CDI, but we should pay attention to antibiotic management and epidemiological surveillance of CDI.

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Comparison of two types of matrix-assisted laser desorption/ionization time-of-flight mass spectrometer for the identification and typing of Clostridium difficile

Cited by 10 publications

References 21 publications

The Epidemiology of Clostridium difficile Infection in Japan: A Systematic Review

The Epidemiology of Clostridium difficile Infection in Japan: A Systematic Review

Head-to-head comparison of Microflex LT and Vitek MS systems for routine identification of microorganisms by MALDI-TOF mass spectrometry in Chile

Toxin genotypes, antibiotic resistance and their correlations in Clostridioides difficile isolated from hospitals in Xi’an, China

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