COMPARISON OF TWO TLC-DPPH<sup>•</sup>-IMAGE PROCESSING PROCEDURES FOR STUDYING FREE RADICAL SCAVENGING ACTIVITY OF COMPOUNDS FROM SELECTED VARIETIES OF <i>MEDICAGO SATIVA</i>

Kowalska, Iwona; Cieśla, Łukasz; Oniszczuk, Tomasz; Waksmundzka‐Hajnos, Monika; Oleszek, Wiesław; Stochmal, Anna

doi:10.1080/10826076.2013.790776

Cited by 18 publications

(7 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The free radical scavenging potential of the pumpkin, zucchini, cucumber, white and yellow pattypan squash preparations was assessed by means of a simple benchtop TLC-DPPH• bioassay with ImageJ program. This method, with small modifications, has been found suitable for the analysis of complex samples, as proved in our previous publications [36][37][38].…”

Section: Tlc-dpph• Testsupporting

confidence: 57%

Comparative Phytochemical, Antioxidant and Haemostatic Studies of Preparations from Selected Vegetables from Cucurbitaceae Family

et al. 2020

Self Cite

View full text Add to dashboard Cite

The aim of this study was to provide detailed insight into the chemical composition and activity of five cucurbit vegetable preparations (pumpkin, zucchini, cucumber, white and yellow pattypan squash), each containing various phytochemical compounds with potential use against oxidative stress induced by the hydroxyl radical donors in human plasma in vitro. We studied the antiradical capacity of vegetable preparations using the DPPH (2,2-diphenyl-1-picrylhydrazyl) method. As oxidative stress may induce changes in hemostasis, our aim included the determination of their effect on three selected hemostatic parameters of plasma, which are three coagulation times: PT (prothrombin time), APTT (activated partial thromboplastin time) and TT (thrombin time). However, none of used vegetable preparations changed APTT, PT or TT compared to the control. The phytochemical composition of the tested preparations was determined by UPLC-ESI-QTOF-MS. In our in vitro experiments, while all five tested preparations had antioxidant potential, the preparation from yellow pattypan squash showed the strongest potential. All cucurbit vegetable preparations inhibited lipid peroxidation. Only zucchini did not have an effect on protein carbonylation and only yellow pattypan squash inhibited thiol oxidation. The antioxidant activity of cucurbits appears to have triggered significant interest in multiple applications, including CVDs (cardiovascular diseases) associated with oxidative stress, which can be treated by supplementation based on these vegetables.

show abstract

Section: Tlc-dpph• Testsupporting

confidence: 57%

Comparative Phytochemical, Antioxidant and Haemostatic Studies of Preparations from Selected Vegetables from Cucurbitaceae Family

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The results of TLC-DPPH tests were recorded in the form of JPG documents. For further analysis, we applied the computer software Sorbfil TLC Videodensitometer (Sorbfil, Sorb Polymer Krasnodar, Russia) [ 36 , 37 ]. The TLC-DPPH assay showed the antiradical activity of the analyzed extracts in relation to the activity of 0.1-mg/mL rutin solution (activity of rutin solution equal to “1”).…”

Section: Methodsmentioning

confidence: 99%

Polyphenol Composition and Antioxidant Potential of Instant Gruels Enriched with Lycium barbarum L. Fruit

et al. 2020

Self Cite

View full text Add to dashboard Cite

Goji fruit (Lycium barbarum L.) has been identified as a polyphenolic compound plant source of noted richness. It also contains polysaccharides, carotenoids, vitamins and minerals, fatty and organic acids. The purpose of the presented research was to produce innovative instant corn gruels with various dry goji berry contents (1, 3 and 5%), to determine the level of included polyphenolic compounds (including individual free phenolic acids) and to assess the antioxidant properties of these functional-food products. A further objective was to identify the optimum value of one of the most important production parameter, the rotational speed of the extruder’s screw during gruel processing. The undertaken chromatographic analysis (LC-ESI-MS/MS) showed a wide variety of available phenolic acids. In the samples with 5% addition of fruit, eight phenolic acids were detected, whereas in the corn gruel without additives, only five were noted. The antioxidant activity, the content of free phenolic acids and the sum of polyphenols increased with increase of the functional additive. For all goji content, screw speeds of 100 and 120 rpm rather than 80 rpm resulted in higher polyphenol amounts and greater Trolox equivalent antioxidant capacity, as well as higher ability to scavenge DPPH.

show abstract

“…Though Sorbfil TLC Video densitometer software is of limited power in signal profiling and data exporting, yet it can be very useful in quantitation and peak identification . Multivariate analysis performed on data based on peak area of characteristic bands of HPTLC chromatograms photographed under UV light at 254 nm was used for quantitative evaluations …”

Section: Methodsmentioning

confidence: 99%

Rapid discrimination of different Apiaceae species based on HPTLC fingerprints and targeted flavonoids determination using multivariate image analysis

Shawky

Kheir

2018

Phytochemical Analysis

View full text Add to dashboard Cite

show abstract

COMPARISON OF TWO TLC-DPPH^•-IMAGE PROCESSING PROCEDURES FOR STUDYING FREE RADICAL SCAVENGING ACTIVITY OF COMPOUNDS FROM SELECTED VARIETIES OF MEDICAGO SATIVA

Cited by 18 publications

References 11 publications

Comparative Phytochemical, Antioxidant and Haemostatic Studies of Preparations from Selected Vegetables from Cucurbitaceae Family

Comparative Phytochemical, Antioxidant and Haemostatic Studies of Preparations from Selected Vegetables from Cucurbitaceae Family

Polyphenol Composition and Antioxidant Potential of Instant Gruels Enriched with Lycium barbarum L. Fruit

Rapid discrimination of different Apiaceae species based on HPTLC fingerprints and targeted flavonoids determination using multivariate image analysis

Contact Info

Product

Resources

About

COMPARISON OF TWO TLC-DPPH•-IMAGE PROCESSING PROCEDURES FOR STUDYING FREE RADICAL SCAVENGING ACTIVITY OF COMPOUNDS FROM SELECTED VARIETIES OF MEDICAGO SATIVA

Cited by 18 publications

References 11 publications

Comparative Phytochemical, Antioxidant and Haemostatic Studies of Preparations from Selected Vegetables from Cucurbitaceae Family

Comparative Phytochemical, Antioxidant and Haemostatic Studies of Preparations from Selected Vegetables from Cucurbitaceae Family

Polyphenol Composition and Antioxidant Potential of Instant Gruels Enriched with Lycium barbarum L. Fruit

Rapid discrimination of different Apiaceae species based on HPTLC fingerprints and targeted flavonoids determination using multivariate image analysis

Contact Info

Product

Resources

About

COMPARISON OF TWO TLC-DPPH^•-IMAGE PROCESSING PROCEDURES FOR STUDYING FREE RADICAL SCAVENGING ACTIVITY OF COMPOUNDS FROM SELECTED VARIETIES OF MEDICAGO SATIVA