Comparison of Two Methods of Pancreas Islets Immunoisolation

Orłowski, T; Sitarek, E; Tatarkiewicz, Krystyna; Sabat, M; Antosiak, Magda

doi:10.1177/039139889702001209

Cited by 6 publications

(8 citation statements)

References 4 publications

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“…Their identity was confirmed by dithizone staining. One day after isolation the islets cultured at 37°C in a humidified atmosphere of 5% CO 2 were encapsulated by Sun's APA method (2,7). Briefly, handpicked islets were suspended in 1 ml of 1.5% (wt/vol) sodium alginate (Sigma, St. Louis, MO, U.S.A.) and droplets formed by an air‐jet‐syringe pump droplet generator were gelled in 1.1% CaCl 2 solution, exposed successively to 0.05% poly L‐lysine (Sigma) and to 0.15% sodium alginate.…”

Section: Methodsmentioning

confidence: 99%

“…Finally, alginate gel inside microcapsules was liquefied by 0.05 M sodium citrate. The formed APA microcapsules (7) were spherical and smooth, with reproducible diameters ranging from 500 to 600 microns, and contained one or two islets each. They all exhibited selective permeability, and were impermeable to albumins and globulins, as well as to the cells.…”

Section: Methodsmentioning

confidence: 99%

“…The most commonly used are immunoprotective membranes, composed of three layers—alginate/poly L‐lysine/alginate (APA) (2,3). Islets encapsulated with APA, grafted intraperitoneally to NOD and to mice with chemically‐induced diabetes (4–7), autoimmune diabetic rats (8), and cynomologus monkeys (9), showed reverse hyperglycemia for several months.…”

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confidence: 99%

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The Influence of Intraperitoneal Transplantation of Free and Encapsulated Langerhans Islets on the Second Set Phenomenon

Orłowski¹,

Godlewska²,

Mościcka³

et al. 2003

Artificial Organs

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To protect the allografts or xenografts against transplant rejection special semipermeable membranes are applied. So far, there are only a few studies on the influence of an immunoisolated graft on the recipient immune system. Therefore, the possibility that an intraperitoneally grafted alginate/poly L-lysine/alginate (APA) coated pancreatic islets graft can effectively sensitize the recipient and provoke second set phenomenon was studied. C3H male mice and male WAG rats were used as donors of full-thickness skin and of free or encapsulated islet intraperitoneal grafts. Male BALB/c mice served as recipients. Skin grafts were performed following the method of Billingham and Medawar. The length of the second skin graft survival time served as the criterion for the sensitizing capacity of the primary graft. APA encapsulation of islets delayed but has not prevented the development of the second set phenomenon. However, the second skin graft rejection time was significantly longer after grafting of encapsulated islets than after free islets transplantation. APA microencapsulation of intraperitoneally transplanted islets delayed but did not prevent the development of the second set phenomenon. Encapsulation does not ensure complete immunoisolation, but only creates "an artificially immunoprivileged site of transplantation."

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The Influence of Intraperitoneal Transplantation of Free and Encapsulated Langerhans Islets on the Second Set Phenomenon

Orłowski¹,

Godlewska²,

Mościcka³

et al. 2003

Artificial Organs

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“…Due to the increasing morbidity and even mortality brought about by diabetes mellitus, a number of studies have been conducted focusing on engineering pancreatic islets [47–49]. We have previously reported that artificial islets can be manufactured from a single cell type, the rat insulin-secreting cell line (RIN-5F), or by coculturing two different cell lines such as RIN-5F and Hep-G2 under shaking mechanism during the culture period [50, 51].…”

Section: Reviewmentioning

confidence: 99%

Applications and Implications of Heparin and Protamine in Tissue Engineering and Regenerative Medicine

Nemeno

Lee

Yang

et al. 2014

BioMed Research International

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Drug repositioning is one of the most rapidly emerging fields of study. This concept is anchored on the principle that diseases have similar damaged or affected signaling pathways. Recently, drugs have been repositioned not only for their alternative therapeutic uses but also for their applications as biomaterials in various fields. However, medical drugs as biomaterials are rarely focused on in reviews. Fragmin and protamine have been recently the sources of increasing attention in the field of tissue engineering and regenerative medicine. Fragmin and protamine have been manufactured primarily as a safe antidote for the circulating heparin. Lately, these drugs have been utilized as either micro- or nanoparticle biomaterials. In this paper, we will briefly describe the concept of drug repositioning and some of the medical drugs that have been repurposed for their alternative therapeutic uses. Also, this will feature the historical background of the studies focused on fragmin/protamine micro/nanoparticles (F/P M/NPs) and their applications as biomaterials in tissue engineering, stem cell therapy, and regenerative medicine.

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“…Der [46]. Es bleibt abzuwarten, inwieweit die aktuelle Problematik der Prionen-assoziierten Krankheiten sowie Retrovirus-Infektionen relevant für diese Entwicklung sein wird [55].…”

Section: Zellen Menschlichen Ursprungsunclassified

Tissue Engineering: Herausforderung für in-vitro Methodenentwicklung

Kirkpatrick¹,

Unger²,

Krump‐Konvalinkova³

et al. 2002

BIOmaterialien

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Schlagworte:Tissue Engineeringin-vitro-Methoden -Entzündung -Angiogenese -Stammzellen -Drug-Delivery Herausforderung für in-vitro Methodenentwicklung Künftige Erfolge (oder Misserfolge) des Tissue Engineering hängen von zwei wesentlichen Aufgaben ab: Funktionelle Implantate müssen erzielt werden und die Biomedizin muss die Auswirkungen der Implantation steuern. Zur Bewerkstelligung funktioneller Implantate stehen verschiedene Systeme zur Verfügung, ζ. B. die Implantation von Zellen, die Zugabe von bioaktiven Substanzen wie Wachstumsfaktoren und andere. Bezüglich der Matrixauswahl ist es möglich, ein permanentes Scaffold oder besser eine biodegradable Matrix, deren Abbauprodukte biokompatibel sind, anzuwenden. Probleme bezüglich Tissue Engineering-Anwendung en sind fehlende Kenntnisse über optimale Ausmaße einer Entzündungs-sowie Reparationsreaktion auf Implantation, um eine effektive Biokompatibilität zu erzielen. Es ist nötig, verfeinerte in-vitro-Methoden zu entwickeln, nicht nur für funktionelle Implantate, sondern auch, um die Komplexität der körperlichen Reaktion auf diese verstehen und steuern zu können. Neben pathobiologischer Grundlage solcher Reaktionen werden hier Prinzipien zur rationalen Gestaltung von in vitro-Prüfmethoden erörtert und Beispiele aus Literatur und Praxis vorgestellt.

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Comparison of Two Methods of Pancreas Islets Immunoisolation

Cited by 6 publications

References 4 publications

The Influence of Intraperitoneal Transplantation of Free and Encapsulated Langerhans Islets on the Second Set Phenomenon

The Influence of Intraperitoneal Transplantation of Free and Encapsulated Langerhans Islets on the Second Set Phenomenon

Applications and Implications of Heparin and Protamine in Tissue Engineering and Regenerative Medicine

Tissue Engineering: Herausforderung für in-vitro Methodenentwicklung

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