Comparison of two methods for obtaining and transporting corneal samples in suspected infectious keratitis

Chang-Sotomayor, M.; Bellés, V. Llorens; Latasiewicz, Marta; Torras-Sanvicens, Josep; Blanco-Domínguez, Irene; Sabater-Cruz, Noelia; Maza, Maite Sainz de la; Bosch-Mestres, Jordi; Palma-Carvajal, F

doi:10.1016/j.jfo.2019.10.010

Cited by 4 publications

(5 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is therefore not possible in this study to assess whether taking the E‐Swab™ second increased the chances of detecting a positive corneal culture. Two previous human studies compared paired corneal culture results from the same eye obtained by either needle or surgical blade scraping and ESwab™ sampling and reported the results obtained with the ESwab™ were not influenced by the collection sequence 20,22 . However Chang‐Sotomayor et.…”

Section: Discussionmentioning

confidence: 99%

“…Two previous human studies compared paired corneal culture results from the same eye obtained by either needle or surgical blade scraping and ESwab™ sampling and reported the results obtained with the ESwab™ were not influenced by the collection sequence. 20,22 However Chang-Sotomayor et. al (2020) reported the positivity rate was significantly higher when the needle scraping was performed first.…”

Section: T a B L Ementioning

confidence: 99%

“…al (2020) reported the positivity rate was significantly higher when the needle scraping was performed first. 20 Future prospective studies randomizing the order of corneal sampling would be required to ascertain whether this affects the ability of either swab types used in this study to detect a positive corneal culture when combined.…”

Section: T a B L Ementioning

confidence: 99%

“…18 The ESwab™ has been demonstrated to be appropriate for multiple applications for use in aerobic and anaerobic culture and viral PCR testing in human patients. [19][20][21] More specifically, the ESwab™ has been demonstrated to be effective in use in corneal culture testing in human patients with suspected infectious keratitis. 22 The use of the ESwab™ has also been demonstrated in multiple veterinary species for nasal, perineal, fecal, and corneal sampling.…”

Section: Introductionmentioning

confidence: 99%

“…The ESwab™ is a Nylon® tipped, flocked swab with 1 mL of liquid Amies medium in a plastic, screw cap tube (Figure 1B). 18 The ESwab™ has been demonstrated to be appropriate for multiple applications for use in aerobic and anaerobic culture and viral PCR testing in human patients 19–21 . More specifically, the ESwab™ has been demonstrated to be effective in use in corneal culture testing in human patients with suspected infectious keratitis 22 .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Comparison of corneal culture results obtained using theESwab™ and Amies charcoal swab in canine and feline patients

Large¹,

Mack²,

Villiers³

et al. 2022

Veterinary Ophthalmology

View full text Add to dashboard Cite

Objective The objective of the study was to compare corneal culture results using the ESwab™ and Amies charcoal swab. Animals studied One hundred fourteen canine and fifteen feline eyes. Procedures Retrospective analysis of Dick White Referrals bacterial and fungal corneal culture data was conducted. Results were included from canine and feline patients, which presented with suspected infectious keratitis that had samples taken using an Amies charcoal swab followed by an ESwab™ in the same eye. In respect to positive and negative cultures, a McNemar test was conducted comparing instances of disagreement between swab types, and the Kappa coefficient (κ) was calculated to assess the level of agreement between swab types. Results The ESwab™ produced more positive corneal cultures (71/129 [55.0%]) than the Amies charcoal swab (41/129 [31.8%]). 2/129 eyes produced positive fungal cultures. Considering 37/129 eyes in which both swab types detected a positive corneal culture, the same bacterial species were cultured from each swab type in 34/37 (91.9%) eyes, and an additional bacterial species was cultured by the ESwab™ in 3/37 (8.1%) eyes. In 34/38 (89.5%), instances of disagreement between swab types, the ESwab™ showed a positive culture, and the Amies charcoal swab showed a negative culture from the same eye, and this difference was significant (p < 0.0001). There was a moderate level of agreement between results from both swab types (κ = 0.432). Conclusions ESwab™ sampling alone may be superior to Amies charcoal swabs for detecting presence of bacteria in suspected infectious keratitis in cats and dogs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: T a B L Ementioning

confidence: 99%

Section: T a B L Ementioning

confidence: 99%