2009
DOI: 10.1016/j.vetpar.2009.07.010
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Comparison of two immunochromatographic assays and the indirect immunofluorscence antibody test for diagnosis of Trypanosoma cruzi infection in dogs in south central Louisiana

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Cited by 57 publications
(64 citation statements)
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“…T. cruzi infection in domestic dogs has been reported widely throughout the southern United States since 1972 (Fig. 4C) (18,20,23,41,105,134,150,189,205,206,239,274,287,298,312). Infection has been documented in at least 48 different breeds in the United States, with the sporting and working breeds accounting for the majority of cases, presumably due to greater exposure to infected vectors and mammalian tissues (150,246).…”
Section: Domestic and Exotic Animal Infections In The United Statesmentioning
confidence: 99%
“…T. cruzi infection in domestic dogs has been reported widely throughout the southern United States since 1972 (Fig. 4C) (18,20,23,41,105,134,150,189,205,206,239,274,287,298,312). Infection has been documented in at least 48 different breeds in the United States, with the sporting and working breeds accounting for the majority of cases, presumably due to greater exposure to infected vectors and mammalian tissues (150,246).…”
Section: Domestic and Exotic Animal Infections In The United Statesmentioning
confidence: 99%
“…Enrollment criteria for our study included dogs >6 months of age within 4 days of admittance to shelters. We detected exposure to T. cruzi using Chagas STAT-PAK, a commercially available rapid immunochromatographic test (Chembio Diagnostic Systems, Inc., Medford, NY, USA) that has been validated for use in dogs ( 9 ) and has high sensitivity and specificity when compared with conventional serologic techniques ( 10 ). To detect parasite DNA within blood, we followed this process: a 200-μL aliquot of centrifuged blood, including the buffy coat, from a subset of seropositive and seronegative dogs across all shelters was subjected to DNA extraction and used as the template in a real-time quantitative PCR with a TaqMan probe to amplify a 166-bp repetitive sequence of satellite DNA specific to T. cruzi ( 11 ).…”
Section: The Studymentioning
confidence: 99%
“…T. cruzi -Detect is rapid and easy to use and it has been suggested as a good screening tool for T. cruzi infection in dogs in the field. [81417] However serological tests should be coupled to confirmatory tests. [1718] The observed prevalence using OligoC-TesT was lower (8.8%; 9/102).…”
Section: Resultsmentioning
confidence: 99%
“…[81417] However serological tests should be coupled to confirmatory tests. [1718] The observed prevalence using OligoC-TesT was lower (8.8%; 9/102). It is however remarkable that 66.7% of the samples tested (68/102) gave “invalid” results.…”
Section: Resultsmentioning
confidence: 99%