Comparison of Two Freezing Extenders and their Influence on Sperm Quality of Tunisian Arab stallions

Najjar, A.; S, Ben Saïd; Kalamoun, S.; Benaoun, B.; Ezzaouia, M.; Chetoui, Cheher; S, Ben Haj Aissa

doi:10.15744/2348-9790.4.302

Cited by 2 publications

(2 citation statements)

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“…Spermatozoa with defects can include tail defects that interferes in spermatozoa motility, than a sperm with low defects rate provides more spermatozoa able to move around. High correlation with hypoosmotic test is in accordance with the fact live spermatozoa respond to the test and have no plasma membrane damage (Najjar et al, 2017). Then the more spermatozoa responding to hypoosmotic test, more live spermatozoa with motility.High correlation with trypan blue intact acrosome (TBlia and TBdia), could be considered favorable because when is detected good motility and the stallions with this characteristic is selected to reproduction, they are indirectly selected by another characteristic that is important.…”

Section: Discussionsupporting

confidence: 63%

Pantaneiro stallions: did the environment influence seminal traits and testicular biometry?

Ribas,

Rua,

Silva

et al. 2024

Preprint

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The aim of this present study was to evaluate the testis biometry and seminal characteristicsof the Pantaneiro horse breed in two season of year. Th experiment was performed in Pantanal region of Brazil, with evaluation of seven Pantaneiro stallions aged from 4 to 12 years and raised in extensive system. Testis traits and semen of the stallions were collected during the rainy season (RS - April) and dry season (DS - October).Seminalvolume, sperm concentration, total number of spermatozoa (TNSTZ), motility, vigor, sperm major defects, sperm total defects, hypoosmotic swelling test, trypan blues test and pH were evaluated. The right and left testicular (RTL and LTL), the right and left testicular height (RTH and LTH) and the right and left testicular width (RTW and LTW) were measured and testicle volume (TV) was calculated. Statistical analysis was performed to verify the effect of season on testis biometry traits and semen traits.Correlations of all characteristics were calculated.There was effect of season on TNSTZ and sperm major defects. All others war not different between RS and DS. The testicular volume means was 179.4 ± 8.6 cm3. There was no effect of season on testicular biometry traits.High and medium correlations were observed among testis length (TL), testis width (TW), TV with TNSTZ. Motility had high correlation with hypoosmotic test. High magnitude correlation was also observed between hypoosmotic test and intact membrane on trypan blue test. It suggests that the integrity of the membrane is essential for a good function of the kinetic of the spermatozoa.The results showed that the Pantaneiro stallions is adapted to the specific tropical environment and not presenting reproductive seasonality, being able to have your semen collected during the entire year without affect its quality also without testicular variation. Furthermore,the testicular length, width and testis volume showed highly representative as a criterion to selection of Pantaneiro stallions to reproduction.

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Section: Discussionsupporting

confidence: 63%

Pantaneiro stallions: did the environment influence seminal traits and testicular biometry?

Ribas,

Rua,

Silva

et al. 2024

Preprint

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“…Ascorbic acid in semen diluents can boost sperm performance by protecting sperm cells from reactive oxidation (Lecewicz et al, 2018). Several studies have shown that the addition of ascorbic acid to diluents improves the quality of frozen semen in bovine (Priyanto et al, 2023) buffalo, ram (Bintara et al, 2023), and rabbit (Najjar et al, 2016) semen. This is because ascorbic acid reduces the occurrence oflipid peroxidation reactions in the plasma membrane of spermatozoa cells.…”

Section: Fresh Semen Qualitymentioning

confidence: 99%

Comparison Enzymatic and Non-Enzymatic Antioxidant in Sperm Quality of Garut Ram Chilled Semen to Enhance Rural Livestock Commodity

Nurlatifah,

Hafid,

Sitaresmi

et al. 2023

AAVS

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The objective of this study was to compare the effects of enzymatic (EZ) and non-enzymatic (NEZ) antioxidants on the quality of spermatozoa in chilled, stored liquid semen from Garut rams. The rams were randomly divided into seven treatments in a 2 × 3 factorial design. The two factors were the antioxidant levels of EZ (25, 50, and 75 mg) and NEZ (5, 10, and 20 mg). Motility, viability, and plasma membrane integrity were the metrics used in this analysis. The experiment conducted with the additional NEZ on the 4 th day of observation showed that the addition of ascorbic acid as NEZ at a dose of 20 mg/100 ml into the egg yolk tris extender resulted in the greatest preservation of ram sperm quality (P<0.05), such as motility and viability. Hence, the addition of catalase as EZ at a dose of 50 mg/100 ml to the egg yolk tris extender resulted in the highest (P<0.05) percentage of motility, percentage of live spermatozoa, and intact plasma membrane. The NEZ group showed better results (P<0.05) than the NEZ group in maintaining motility, viability, and membrane integrity. The research concluded that the administration of NEZ at a dose of 20 mg/100 ml extender and EZ treatment at 50 mg/100 ml extender represents the optimal dose to preserve sperm quality until day four of dilution. In addition, the NEZ antioxidant showed better-improved quality of spermatozoa in the liquid semen of Garut rams compared to the EZ antioxidant.

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Comparison of Two Freezing Extenders and their Influence on Sperm Quality of Tunisian Arab stallions

Cited by 2 publications

References 0 publications

Pantaneiro stallions: did the environment influence seminal traits and testicular biometry?

Pantaneiro stallions: did the environment influence seminal traits and testicular biometry?

Comparison Enzymatic and Non-Enzymatic Antioxidant in Sperm Quality of Garut Ram Chilled Semen to Enhance Rural Livestock Commodity

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