Comparison of two encapsulation processes to protect the commensal gut probiotic bacterium Faecalibacterium prausnitzii from the digestive tract

Raise, Audrey; Dupont, Sébastien; Iaconelli, Cyril; Caliri, Christian; Charriau, Alexandre; Gervais, Patrick; Chambin, Odile; Beney, Laurent

doi:10.1016/j.jddst.2020.101608

Cited by 20 publications

(10 citation statements)

References 55 publications

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“…However, these researchers highlighted the need to find alternatives to anaerobic storage as well as the urgency to develop an optimal coating to protect bacteria against gastric acidity. Taking this in consideration, Raise et al (2020) designed and compared two encapsulation processes that could deliver bacteria in a viable and functional form to the intestine. One process consisted of mixing previously freeze-dried F. prausnitzii with a melted hydrophobic matrix (Gelucire® 43/01) to encapsulate them into a solid lipid base after cooling.…”

Section: Production Constraints and Crafty Delivery Systemsmentioning

confidence: 99%

Next-generation probiotics

et al. 2022

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In the last years, the scientific community has recognized that specific microbial strains resident in the intestinal ecosystem play a key role in human health, participating in several functions beneficial to the host. Such microorganisms have been termed as next-generation probiotics and they are presently considered as food/nutraceutical supplements and biotherapeutic products. However, most of the next-generation probiotic candidates are nutritionally demanding and highly sensitive to aerobic conditions, which translates into several technological challenges concerning large-scale production and appeals to the development of suitable delivery systems able to promote viability and functionality of such probiotic strains. In this chapter, we will present an overall perspective of next-generation probiotics candidates in terms of their health beneficial effects, the delivery systems developed and employed to protect them, and related regulation framework and risk assessment targeting relevant criteria for commercialization in food and pharmaceutical markets.

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Section: Production Constraints and Crafty Delivery Systemsmentioning

confidence: 99%

Next-generation probiotics

et al. 2022

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“…One method for maintaining bacterial viability is bacterial encapsulation. Bacterial encapsulation is the process of wrapping (coating) bacteria with materials that help to maintain their viability and protect them from unfavorable environmental factors such as heat and chemicals (Raise et al, 2020;Ayama et al, 2014;Mahmoud et al, 2020). Bacterial viability is the ability of bacterial cells to grow normally under optimal conditions (Both et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Proteus penneri encapsulation with maltodextrin and sodium alginate using a spray-drying method

2022

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Proteus penneri has been isolated from the digestive tract and used for coffee fermentation. As its viability is compromised rapidly by unfavorable environmental conditions, encapsulation technique is used to protect it. The purpose of this study is to develop a bacterial encapsulation method that can preserve viability of Proteus penneri. The Proteus penneri were isolated from civet feces. Bacterial encapsulation was performed using a spray-drying method, and maltodextrin and sodium alginate were used as coating materials. We tested 15%, 20%, and 30% (w/v) concentrations of maltodextrin and 0.50%, 0.75%, and 1% (w/v) concentrations of sodium alginate. The encapsulated bacteria were stored for two weeks to study the stability of enzyme activity. We found that the spray-drying technology using maltodextrin as coating material resulted in a stable encapsulated bacterium. However, Proteus penneri did not survive well during the process. Loss of viability is caused by the membrane cell wall damage. After two weeks storage, bacterial viability decreased significantly. The enzymatic activity of the encapsulated cellulolytic bacteria was influenced by concentration of the bacteria, however the activity appeared to be stable after two weeks of storage.

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“… 13 , 18 Lipids, fats, oils, waxes, and resins are another category of biomaterials considered as a favorable matrix for encapsulation of probiotics. 19 , 20 …”

Section: Introductionmentioning

confidence: 99%

“…Protein-based encapsulated probiotic formulations are composed of several animal- and plant-derived proteins, such as gluten, zein, gelatin, and milk proteins. Probiotic encapsulation in plant-derived vegan proteins is being considered as an excellent alternative to animal-derived proteins. , Lipids, fats, oils, waxes, and resins are another category of biomaterials considered as a favorable matrix for encapsulation of probiotics. , …”

Section: Introductionmentioning

confidence: 99%

“…13,18 Lipids, fats, oils, waxes, and resins are another category of biomaterials considered as a favorable matrix for encapsulation of probiotics. 19,20 However, encapsulation systems based on conventional biopolymers, such as alginate, have some limitations in protecting the probiotics from gastric fluids. 21−25 Cellulosebased hydrogels on their own or in combination with other biopolymers have recently shown great promise to overcome the limitations of conventional biopolymer-based encapsulation systems.…”

Section: ■ Introductionmentioning

confidence: 99%

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Perspective on Constructing Cellulose-Hydrogel-Based Gut-Like Bioreactors for Growth and Delivery of Multiple-Strain Probiotic Bacteria

Mettu

Hathi

Athukoralalage

et al. 2021

J. Agric. Food Chem.

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The current perspective presents an outlook on developing gut-like bioreactors with immobilized probiotic bacteria using cellulose hydrogels. The innovative concept of using hydrogels to simulate the human gut environment by generating and maintaining pH and oxygen gradients in the gut-like bioreactors is discussed. Fundamentally, this approach presents novel methods of production as well as delivery of multiple strains of probiotics using bioreactors. The relevant existing synthesis methods of cellulose hydrogels are discussed for producing porous hydrogels. Harvesting methods of multiple strains are discussed in the context of encapsulation of probiotic bacteria immobilized on cellulose hydrogels. Furthermore, we also discuss recent advances in using cellulose hydrogels for encapsulation of probiotic bacteria. This perspective also highlights the mechanism of probiotic protection by cellulose hydrogels. Such novel gut-like hydrogel bioreactors will have the potential to simulate the human gut ecosystem in the laboratory and stimulate new research on gut microbiota.

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Comparison of two encapsulation processes to protect the commensal gut probiotic bacterium Faecalibacterium prausnitzii from the digestive tract

Cited by 20 publications

References 55 publications

Next-generation probiotics

Next-generation probiotics

Proteus penneri encapsulation with maltodextrin and sodium alginate using a spray-drying method

Perspective on Constructing Cellulose-Hydrogel-Based Gut-Like Bioreactors for Growth and Delivery of Multiple-Strain Probiotic Bacteria

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