Comparison of Two Common Outpatient Preparations for Colonoscopy in Children and Youth

Jiménez-Rivera, Carolina; Haas, Donna; Boland, Margaret; Barkey, Janice L.; Mack, David R.

doi:10.1155/2009/518932

Cited by 21 publications

(16 citation statements)

References 17 publications

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“…When sodium picosulphate is used, the rate of AEs in single studies seems to be lower 18 22. In direct comparison, sodium picosulphate was better tolerated than PEG 20 21 with a significantly lower need for nasogastric tube insertion corresponding to our results 20.…”

Section: Resultssupporting

confidence: 66%

Bowel preparation in pediatric colonoscopy: results of an open observational study

Berger¹,

Claßen

Engelhardt³

et al. 2016

Endosc Int Open

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Background and study aims: The goal of this study was to analyze the bowel cleansing methods currently used for pediatric colonoscopy in terms of effectiveness, tolerance and safety. Patients and methods: Data from 768 colonoscopies reported by 28 centers were registered in an online database for further analysis. Binary logistic regression was used to determine how preparation methods affected the cleaning effect (Aronchick score) and the rate of adverse events (Aes) and complications. Results: The most frequently reported cleansing agents were sodium picosulphate (54.2?%) and polyethylene-glycol (41.3?%) in various combinations. The cleaning effect was good to excellent in 72.6?% of patients. AEs during the preparation period occurred in 21.5?% of patients. Complications during endoscopy were reported in 12.1?% and were mostly mild. The different agents had no influence on the cleaning effect. In contrast the risk of AEs during preparation was significantly increased when polyethylene-glycol was used (odds ratio (OR) 2.112, P?=?0.002) but reduced with the use of sodium picosulphate (OR 0.380, P?

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Section: Resultssupporting

confidence: 66%

Bowel preparation in pediatric colonoscopy: results of an open observational study

Berger¹,

Claßen

Engelhardt³

et al. 2016

Endosc Int Open

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“…Colonoscopy preparation was done as per standard protocol modified to one day44. During colonoscopy, once the cecum and proximal ascending colon was reached, any loose fluid and debris was aspirated.…”

Section: Methodsmentioning

confidence: 99%

Altered intestinal microbiota–host mitochondria crosstalk in new onset Crohn’s disease

et al. 2016

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Intestinal microbial dysbiosis is associated with Crohn's disease (CD). However, the mechanisms leading to the chronic mucosal inflammation that characterizes this disease remain unclear. In this report, we use systems-level approaches to study the interactions between the gut microbiota and host in new-onset paediatric patients to evaluate causality and mechanisms of disease. We report an altered host proteome in CD patients indicative of impaired mitochondrial functions. In particular, mitochondrial proteins implicated in H2S detoxification are downregulated, while the relative abundance of H2S microbial producers is increased. Network correlation analysis reveals that Atopobium parvulum controls the central hub of H2S producers. A. parvulum induces pancolitis in colitis-susceptible interleukin-10-deficient mice and this phenotype requires the presence of the intestinal microbiota. Administrating the H2S scavenger bismuth mitigates A. parvulum-induced colitis in vivo. This study reveals that host–microbiota interactions are disturbed in CD and thus provides mechanistic insights into CD pathogenesis.

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“…The protocol was approved by the Research Ethics Board of the Children’s Hospital of Eastern Ontario (CHEO). Colonoscopy preparation was done as per standard protocol modified to 1 day [ 40 ]. During colonoscopy, once the proximal ascending colon was intubated, any fluid and loose debris was aspirated and discarded.…”

Section: Methodsmentioning

confidence: 99%

MetaPro-IQ: a universal metaproteomic approach to studying human and mouse gut microbiota

et al. 2016

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BackgroundThe gut microbiota has been shown to be closely associated with human health and disease. While next-generation sequencing can be readily used to profile the microbiota taxonomy and metabolic potential, metaproteomics is better suited for deciphering microbial biological activities. However, the application of gut metaproteomics has largely been limited due to the low efficiency of protein identification. Thus, a high-performance and easy-to-implement gut metaproteomic approach is required.ResultsIn this study, we developed a high-performance and universal workflow for gut metaproteome identification and quantification (named MetaPro-IQ) by using the close-to-complete human or mouse gut microbial gene catalog as database and an iterative database search strategy. An average of 38 and 33 % of the acquired tandem mass spectrometry (MS) spectra was confidently identified for the studied mouse stool and human mucosal-luminal interface samples, respectively. In total, we accurately quantified 30,749 protein groups for the mouse metaproteome and 19,011 protein groups for the human metaproteome. Moreover, the MetaPro-IQ approach enabled comparable identifications with the matched metagenome database search strategy that is widely used but needs prior metagenomic sequencing. The response of gut microbiota to high-fat diet in mice was then assessed, which showed distinct metaproteome patterns for high-fat-fed mice and identified 849 proteins as significant responders to high-fat feeding in comparison to low-fat feeding.ConclusionsWe present MetaPro-IQ, a metaproteomic approach for highly efficient intestinal microbial protein identification and quantification, which functions as a universal workflow for metaproteomic studies, and will thus facilitate the application of metaproteomics for better understanding the functions of gut microbiota in health and disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s40168-016-0176-z) contains supplementary material, which is available to authorized users.

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Comparison of Two Common Outpatient Preparations for Colonoscopy in Children and Youth

Cited by 21 publications

References 17 publications

Bowel preparation in pediatric colonoscopy: results of an open observational study

Bowel preparation in pediatric colonoscopy: results of an open observational study

Altered intestinal microbiota–host mitochondria crosstalk in new onset Crohn’s disease

MetaPro-IQ: a universal metaproteomic approach to studying human and mouse gut microbiota

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