Comparison of two chemiluminescent immunoassays in the detection of measles IgM antibodies

Gómez-Camarasa, Cristina; Lara-Oya, Ana; Cobo, Fernando; Sampedro-Martínez, Antonio; Rodríguez‐Grangér, Javier; Gutiérrez‐Fernández, José; Navarro-Marí, José María

doi:10.1016/j.jviromet.2016.08.018

Cited by 11 publications

(6 citation statements)

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“…All these serologies were performed with CLIAs which in previous studies displayed high sensitivity and specificity (≥97.6% and ≥96.6%, respectively, for all the serologies). [66–70] …”

Section: Discussionmentioning

confidence: 99%

Infectious diseases prevalence, vaccination coverage, and diagnostic challenges in a population of internationally adopted children referred to a Tertiary Care Children's Hospital from 2009 to 2015

et al. 2017

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Infectious diseases are common in internationally adopted children (IAC).With the objective to evaluate infectious diseases prevalence in a large cohort of IAC and to explore possible risk factors for tuberculosis (TB) and parasitic infections, clinical and laboratory data at first screening visit of all IAC (<18 years) consecutively referred to our Center in 2009 to 2015 were collected and analyzed.In total, 1612 children (median age: 5.40 years; interquartile range: 3.00–7.90) were enrolled, 123/1612 (7.60%) having medical conditions included in the special needs definition. The most frequent cutaneous infections were Molluscum contagiosum (42/1612; 2.60%) and Tinea capitis (37/1612; 2.30%). Viral hepatitis prevalence was <1% (hepatitis B virus [HBV]: 13 children, 0.80%; hepatitis C virus: 1 child, 0.10%; hepatitis A virus: 6 children, 0.40%). A parasitic infection was diagnosed in 372/1612 (23.10%) children. No risk factors for parasitosis were evidenced. Active TB was diagnosed in 4/1355 (0.3%) children, latent TB in 222/1355 (16.40%). Only 3.7% (51/1355) children had concordant positive tuberculin skin test (TST) and QuantiFERON-TB-Gold In-Tube (QFT-G-IT) results. Risk factors for TST+/QFT-G-IT− results were previous Bacille de Calmette-Guérin vaccination (adjusted odds ratio [aOR]: 2.18; 96% confidence interval [CI]: 1.26–3.79; P = 0.006), and age ≥5 years (aOR: 1.49; 95% CI: 1.06–2.11; P = 0.02). The proportion of children with nonprotective titers for vaccine-preventable diseases (VPD) ranged from 15.70% (208/1323) for tetanus to 35.10% (469/1337) for HBV.Infectious diseases were commonly observed in our cohort. The high rate of discordant TST/QFT-G results brings up questions regarding the optimal management of these children, and suggests that, at least in children older than 5 years, only QFT-G-IT results may be reliable. The low proportion of children protected for VPD, confirms importance of a timely screening.

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Section: Discussionmentioning

confidence: 99%

Infectious diseases prevalence, vaccination coverage, and diagnostic challenges in a population of internationally adopted children referred to a Tertiary Care Children's Hospital from 2009 to 2015

et al. 2017

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“…Other recently described methods for diagnosis of measles, such as automatable focus reduction neutralization tests (AFRNT) [32], chemiluminescent immunosorbant assays (CLIA) [33], or loopmediated isothermal amplification (LAMP) [34], are used by some laboratories.…”

Section: Potential Other Diagnostic Methodsmentioning

confidence: 99%

Challenges of measles and rubella laboratory diagnostic in the era of elimination

Hübschen

Bork

Brown

et al. 2017

Clinical Microbiology and Infection

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The Member States of the WHO European Region adopted the goal of measles and rubella elimination more than 10 years ago, but so far only 21 of 53 countries have reached this target. Laboratory investigation of suspected cases is essential to support disease elimination efforts. Therefore, WHO maintains a network of accredited laboratories providing high-quality testing. Laboratory investigation heavily relies on specific IgM serology and increasingly on virus detection by reverse transcription (RT)-PCR, but other methods such as IgG avidity testing and genetic characterization of virus strains have gained in importance. In elimination settings, often few samples from suspected cases are available for testing, but testing proficiency must be maintained. The predictive value of an IgM-positive result decreases and other rash-fever disease aetiologies become more important. In addition, cases with a rash after measles/rubella vaccination or with mild disease after waning of vaccine-induced antibodies are seen more often. Thus, it is necessary to perform comprehensive and potentially time-consuming and costly investigations of every suspected case using quality-controlled laboratory methods. At the same time rapid feedback to public health officers is required for timely interventions. The introduction of new laboratory methods for comprehensive case investigations requires training of staff under the supervision of WHO-accredited reference laboratories and the definition of appropriate test algorithms. Clinical, laboratory, and epidemiological data are essential for final case classification and investigation of chains of transmission in the endgame of measles and rubella elimination.

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“…Previous extensive evaluations of commercial measles IgM EIA kits resulted in the broad adoption of the Siemens (previously Behring) Enzygnost kit within the WHO global measles and rubella laboratory network ( 11 – 13 ). More recent evaluations have included automated chemiluminescent methods ( 17 – 21 ), but there is still a need for conventional manual EIA methods, which are recommended within the WHO measles and rubella laboratory network ( 10 ). Prompted by the discontinuation of the Enzygnost kit ( 14 ), six alternative EIA methods and one automated CLIA method were evaluated in this study.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Diagnostic Accuracy of Eight Commercial Assays for the Detection of Measles Virus-Specific IgM Antibodies

et al. 2021

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The World Health Organization (WHO) has targeted measles for global eradication through mass immunization. For effective monitoring of eradication targets, high quality surveillance is needed. The detection of IgM antibodies, specific to the measles virus, with the use of commercial enzyme-linked immunosorbent assays (ELISA or EIA) is broadly used within the WHO global measles and rubella laboratory network for laboratory confirmation, and in particular ELISA kits manufactured by Siemens (Enzygnost® kits) have been primarily used. Spurred by the discontinuation of these kits, this study aims to report on the clinical sensitivity and specificity of comparable commercial ELISA kits and one automated chemiluminescent immunoassay (CLIA) method. A panel of 239 serum samples was assembled that included sera from confirmed measles cases (n=50) and probable post-MMR vaccine response (n=2). Measles negative sera (n=187) were collected from individuals presenting with other fever and rash illnesses. A total of 7 ELISA kits were evaluated (Euroimmun native antigens and recombinant nucleoprotein, IBL, Clin-Tech Microimmune, NovaTec NovaLisa®, Serion and Siemens Enzygnost®) and one CLIA method (DiaSorin LIAISON® XL). The ELISA kits included two IgM capture methods and five indirect methods. Calculated sensitivities and specificities ranged from 75.0% to 98.1% and 86.6% to 99.5% respectively. The parvovirus B19 IgM positive sera were noted to cause false positive results particularly for the ELISA kits from Serion and NovaLisa®; specificities for this subset of samples ranged from 51.4% to 100.0%. The capture IgM ELISA methods provided the best combination of sensitivity and specificity.

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Comparison of two chemiluminescent immunoassays in the detection of measles IgM antibodies

Cited by 11 publications

References 4 publications

Infectious diseases prevalence, vaccination coverage, and diagnostic challenges in a population of internationally adopted children referred to a Tertiary Care Children's Hospital from 2009 to 2015

Infectious diseases prevalence, vaccination coverage, and diagnostic challenges in a population of internationally adopted children referred to a Tertiary Care Children's Hospital from 2009 to 2015

Challenges of measles and rubella laboratory diagnostic in the era of elimination

Evaluation of Diagnostic Accuracy of Eight Commercial Assays for the Detection of Measles Virus-Specific IgM Antibodies

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