Comparison of tumour morphology and structure from U87 and U118 glioma cells cultured on chicken embryo chorioallantoic membrane

Jaworski, Sławomir; Sawosz, Ewa; Grodzik, Marta; Kutwin, Marta; Wierzbicki, Mateusz; Włodyga, Karolina; Jasik, Agnieszka; Reichert, M.; Chwalibog, A.

doi:10.2478/bvip-2013-0101

Cited by 11 publications

(8 citation statements)

References 15 publications

(15 reference statements)

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“…We tested both cell lines because they significantly differed in terms of their morphology, histology, and structure. U87 tumors were almost 3× larger than U118 cells ( Jaworski et al, 2013 ). After the xenograft tumors had grown for 14 days, we divided the mice into two groups.…”

Section: Resultsmentioning

confidence: 89%

Extracts of Artocarpus communis Induce Mitochondria-Associated Apoptosis via Pro-oxidative Activity in Human Glioblastoma Cells

et al. 2018

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Glioblastoma multiforme (GBM) is an extremely aggressive and devastating malignant tumor in the central nervous system. Its incidence is increasing and the prognosis is poor. Artocarpin is a natural prenylated flavonoid with various anti-inflammatory and anti-tumor properties. Studies have shown that artocarpin is associated with cell death of primary glioblastoma cells. However, the in vivo effects and the cellular and molecular mechanisms modulating the anticancer activities of artocarpin remain unknown. In this study, we demonstrated that treating the glioblastoma cell lines U87 and U118 cells with artocarpin induced apoptosis. Artocarpin-induced apoptosis is associated with caspase activation and poly (ADP-ribose) polymerase (PARP) cleavage and is mediated by the mitochondrial pathway. This is associated with mitochondrial depolarization, mitochondrial-derived reactive oxidative species (ROS) production, cytochrome c release, Bad and Bax upregulations, and Bcl-2 downregulation. Artocarpin induced NADPH oxidase/ROS generation plays an important role in the mitochondrial pathway activation. Furthermore, we found artocarpin-induced ROS production in mitochondria is associated with Akt- and ERK1/2 activation. After treatment with artocarpin, ROS causes PI3K/Akt/ERK1/2-induced cell death of these tumor cells. These observations were further verified by the results from the implantation of both U87 and U118 cells into in vivo mouse. In conclusion, our findings suggest that artocarpin induces mitochondria-associated apoptosis of glioma cells, suggesting that artocarpine can be a potential chemotherapeutic agent for future GBM treatment.

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Section: Resultsmentioning

confidence: 89%

Extracts of Artocarpus communis Induce Mitochondria-Associated Apoptosis via Pro-oxidative Activity in Human Glioblastoma Cells

et al. 2018

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“…As a consequence, the nature of the interactions of biological molecules and/or cells with pG is likely to depend on the site of interaction: the surface or edges. Previous findings indicated that glioblastoma cells had a strong affinity for, and adhered to, the surface of pG flakes, rather than the edges [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Interaction of graphene family materials with Listeria monocytogenes and Salmonella enterica

et al. 2015

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Graphene family materials have unique properties, which make them valuable for a range of applications. The antibacterial properties of graphene have been reported; however, findings have been contradictory. This study reports on the antimicrobial proprieties of three different graphene materials (pristine graphene (pG), graphene oxide (GO), and reduced graphene oxide (rGO)) against the food-borne bacterial pathogens Listeria monocytogenes and Salmonella enterica. A high concentration (250 μg/mL) of all the analyzed graphenes completely inhibited the growth of both pathogens, despite their difference in bacterial cell wall structure. At a lower concentration (25 μg/mL), similar effects were only observed with GO, as growth inhibition decreased with pG and rGO at the lower concentration. Interaction of the nanoparticles with the pathogenic bacteria was found to differ depending on the form of graphene. Microscopic imaging demonstrated that bacteria were arranged at the edges of pG and rGO, while with GO, they adhered to the nanoparticle surface. GO was found to have the highest antibacterial activity.

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“…These lines are characterized by different phenotypes and the activity of genes involved in regulation of the cell cycle. 16 The objectives of this study are to measure the toxicity of GO and rGO, and the proapoptotic and necrotic activities of graphene in glioma cells and tumors cultured on chorioallantoic membrane.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo effects of graphene oxide and reduced graphene oxide on glioblastoma

Jaworski¹,

Sawosz²,

Kutwin³

et al. 2015

IJN

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Graphene and its related counterparts are considered the future of advanced nanomaterials owing to their exemplary properties. However, information about their toxicity and biocompatibility is limited. The objective of this study is to evaluate the toxicity of graphene oxide (GO) and reduced graphene oxide (rGO) platelets, using U87 and U118 glioma cell lines for an in vitro model and U87 tumors cultured on chicken embryo chorioallantoic membrane for an in vivo model. The in vitro investigation consisted of structural analysis of GO and rGO platelets using transmission elec tron microscopy, evaluation of cell morphology and ultrastructure, assessment of cell viability by XTT assay, and investigation of cell proliferation by BrdU assay. Toxicity in U87 glioma tumors was evaluated by calculation of weight and volume of tumors and analyses of ultrastructure, histology, and protein expression. The in vitro results indicate that GO and rGO enter glioma cells and have different cytotoxicity. Both types of platelets reduced cell viability and proliferation with increasing doses, but rGO was more toxic than GO. The mass and volume of tumors were reduced in vivo after injection of GO and rGO. Moreover, the level of apoptotic markers increased in rGO-treated tumors. We show that rGO induces cell death mostly through apoptosis, indicating the potential applicability of graphene in cancer therapy.

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Comparison of tumour morphology and structure from U87 and U118 glioma cells cultured on chicken embryo chorioallantoic membrane

Cited by 11 publications

References 15 publications

Extracts of Artocarpus communis Induce Mitochondria-Associated Apoptosis via Pro-oxidative Activity in Human Glioblastoma Cells

Extracts of Artocarpus communis Induce Mitochondria-Associated Apoptosis via Pro-oxidative Activity in Human Glioblastoma Cells

Interaction of graphene family materials with Listeria monocytogenes and Salmonella enterica

In vitro and in vivo effects of graphene oxide and reduced graphene oxide on glioblastoma

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