Comparison of transcriptional and translational changes caused by long-term menadione exposure in Aspergillus nidulans

Pusztahelyi, Tünde; Klement, Éva; Szájli, Emília; Klem, József; Miskei, Márton; Karányi, Zsolt; Emri, Tamás; Kovács, Szilvia; Orosz, Gyula; Kovács, Kornél L.; Medzihradszky, Katalin F.; Prade, Rolf A.; Pócsi, István

doi:10.1016/j.fgb.2010.08.006

Cited by 40 publications

(34 citation statements)

References 87 publications

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“…Furthermore, the induction of alcR was also detected in the presence of ethanol and acetate (Pateman et al, 1983) or hypoxia (Terabayashi et al, 2012), while oxidative stress caused by menadione downregulated this protein (Pusztahelyi et al, 2011). These findings demonstrated the importance of AlcR in the coordination of several stress responses.…”

Section: Changes In Carbon and Nitrogen Metabolismmentioning

confidence: 90%

Transcriptome changes initiated by carbon starvation in Aspergillus nidulans

et al. 2013

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Carbon starvation is a common stress for micro-organisms both in nature and in industry. The carbon starvation stress response (CSSR) involves the regulation of several important processes including programmed cell death and reproduction of fungi, secondary metabolite production and extracellular hydrolase formation. To gain insight into the physiological events of CSSR, DNA microarray analyses supplemented with real-time RT-PCR (rRT-PCR) experiments on 99 selected genes were performed. These data demonstrated that carbon starvation induced very complex changes in the transcriptome. Several genes contributing to protein synthesis were upregulated together with genes involved in the unfolded protein stress response. The balance between biosynthesis and degradation moved towards degradation in the case of cell wall, carbohydrate, lipid and nitrogen metabolism, which was accompanied by the production of several hydrolytic enzymes and the induction of macroautophagy. These processes provide the cultures with longterm survival by liberating nutrients through degradation of the cell constituents. The induced synthesis of secondary metabolites, antifungal enzymes and proteins as well as bacterial cell walldegrading enzymes demonstrated that carbon-starving fungi should have marked effects on the micro-organisms in their surroundings. Due to the increased production of extracellular and vacuolar enzymes during carbon starvation, the importance of the endoplasmic reticulum increased considerably.

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Section: Changes In Carbon and Nitrogen Metabolismmentioning

confidence: 90%

Transcriptome changes initiated by carbon starvation in Aspergillus nidulans

et al. 2013

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“…The transcription profiles of genes identified in highthroughput transcriptomic/proteomic studies on menadione-treated mycelia (Pó csi et al, 2005;Pusztahelyi et al, 2011) changed significantly in hmbBD mycelia. Total GST activity was nearly doubled in hmbBD mycelia and we found that transcription levels of all the known or predicted GST-coding genes, except one (gstB), were upregulated in the hmbBD strain.…”

Section: Discussionmentioning

confidence: 99%

“…The oxidative stress response of hmbBD and hmbB + mycelia was studied after menadione and diamide treatment. The concentrations of the stressors were chosen to match those frequently used in oxidative stress studies of A. nidulans (0.8 mM menadione and 1.8 mM diamide), enabling us to interpret our results in the context of relevant physiological, transcriptomic and proteomic data (Pó csi et al, 2005;Pusztahelyi et al, 2011).…”

Section: Effect Of Menadione and Diamide On The Redox State Of Hmbbd mentioning

confidence: 99%

“…We monitored the relative mRNA levels of gstA (Fraser et al, 2002), gstB (Sato et al, 2009), five putative GST genes (AN10273, AN0815, AN2948, AN5831 and AN6158) and four putative glutaredoxin genes (gpxA, AN3255, AN4215 and AN4304). gstA, gpxA, AN3255, AN4304, GstB and AN10273 were found to be oxidative stress-induced genes/proteins in previous high-throughput studies (Fraser et al, 2002;Pó csi et al, 2005;Pusztahelyi et al, 2011), so we selected them for our investigation. The function of the remaining GST and glutaredoxin genes (AN0815, AN2948, AN5831, AN6158 and AN4215) is unknown, and they were chosen randomly from the AspGD database (Arnaud et al, 2012) for investigation.…”

Section: Effect Of Menadione and Diamide On The Redox State Of Hmbbd mentioning

confidence: 99%

“…We found that glrA expression was elevated by 2.8-fold in hmbBD mycelia, and the expression levels of trxR and catB showed 2.1-and 1.8-fold increases, respectively. TrxR and GstB have also been detected as menadioneresponsive proteins in high-throughput proteomic experiments upon 6 h 0.8 mM menadione treatment (Pusztahelyi et al, 2011) together with FhbA (flavohaemoprotein) and AN2343 (putative nitroreductase). We monitored the mRNA levels of the two latter genes, and found that fhbA and AN2343 transcript levels were 15.7-and 4.3-fold higher in the hmbBD strain than in the hmbB + strain.…”

Section: Effect Of Menadione and Diamide On The Redox State Of Hmbbd mentioning

confidence: 99%

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Further characterization of the role of the mitochondrial high-mobility group box protein in the intracellular redox environment of Aspergillus nidulans

et al. 2015

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zé p fasor 52, Hungary HmbB, a predominantly mitochondrial high-mobility group box (HMGB) protein, of Aspergillus nidulans affects diverse biological activities, such as sterigmatocystin production, the maintenance of mitochondrial DNA copy number, germination of asexual and sexual spores, and protection against oxidative stress agents. We hypothesized that the latter correlates with an unbalanced intracellular redox state, in which case, a not yet fully characterized physiological function could be attributed to this mitochondrial HMGB protein. Here, we studied the intracellular redox environment and oxidative stress tolerance in hmbB + and hmbBD strains under normal and oxidative stress conditions by measuring glutathione redox couple, intracellular reactive oxygen species (ROS) content and ROS-protecting enzyme activities. Our results revealed that the intracellular redox environment is different in hmbBD conidia and mycelia from that of hmbB + , and shed light on the seemingly contradictory difference in the tolerance of hmbBD mycelia to diamide and menadione oxidative stressors.

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Stress tolerances of nullmutants of function‐unknown genes encoding menadione stress‐responsive proteins in Aspergillus nidulans

Leiter

Bálint

Miskei

et al. 2015

J. Basic Microbiol.

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A group of menadione stress-responsive function-unkown genes of Aspergillus nidulans (Locus IDs ANID_03987.1, ANID_06058.1, ANID_10219.1, and ANID_10260.1) was deleted and phenotypically characterized. Importantly, comparative and phylogenetic analyses of the tested A. nidulans genes and their orthologs shed light only on the presence of a TANGO2 domain with NRDE protein motif in the translated ANID_06058.1 gene but did not reveal any recognizable protein-encoding domains in other protein sequences. The gene deletion strains were subjected to oxidative, osmotic, and metal ion stress and, surprisingly, only the ΔANID_10219.1 mutant showed an increased sensitivity to 0.12 mmol l(-1) menadione sodium bisulfite. The gene deletions affected the stress sensitivities (tolerances) irregularly, for example, some strains grew more slowly when exposed to various oxidants and/or osmotic stress generating agents, meanwhile the ΔANID_10260.1 mutant possessed a wild-type tolerance to all stressors tested. Our results are in line with earlier studies demonstrating that the deletions of stress-responsive genes do not confer necessarily any stress-sensitivity phenotypes, which can be attributed to compensatory mechanisms based on other elements of the stress response system with overlapping functions.

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Comparison of transcriptional and translational changes caused by long-term menadione exposure in Aspergillus nidulans

Cited by 40 publications

References 87 publications

Transcriptome changes initiated by carbon starvation in Aspergillus nidulans

Transcriptome changes initiated by carbon starvation in Aspergillus nidulans

Further characterization of the role of the mitochondrial high-mobility group box protein in the intracellular redox environment of Aspergillus nidulans

Stress tolerances of nullmutants of function‐unknown genes encoding menadione stress‐responsive proteins in Aspergillus nidulans

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