Comparison of toxicities of moxifloxacin, cefuroxime, and levofloxacin to corneal endothelial cells in vitro

Haruki, Tomoko; Miyazaki, Dai; Matsuura, Kazuki; Terasaka, Yuki; Noguchi, Yumiko; Inoue, Yoshitsugu; Yamagami, Satoru

doi:10.1016/j.jcrs.2014.08.027

Cited by 22 publications

(10 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moxifloxacin also had direct toxic effects to other ocular cells. For example, corneal endothelial cells exposed to moxifloxacin caused cell damage with a reduction of intrinsic esterase activity 16 . An intracamerally injection of moxifloxacin can cause oxidative stress and caspase activation in corneal cells 17 .…”

Section: Discussionmentioning

confidence: 99%

Toxicities of and inflammatory responses to moxifloxacin, cefuroxime, and vancomycin on retinal vascular cells

Miyake

Miyazaki

Shimizu

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Prophylactic intracameral injection of antibiotics is commonly used to prevent endophthalmitis after cataract surgery. However, devastating visual complications have been reported including hemorrhagic occlusive retinal vasculitis (HORV).To determine the toxic and inflammatory effects of moxifloxacin, cefuroxime, and vancomycin on human retinal vascular cells, human retinal vascular endothelial cells (RVEC) and pericytes were exposed to three antibiotics, and the adverse effects were assessed by membrane damage, loss of intrinsic esterase activity, kinetic cell viability, and inflammatory cytokine secretion. Their retinal toxicity was examined by live/dead assays after an intravitreal injection of the three antibiotics into mice eyes. In vascular cells in culture, membrane damage and loss of esterase activity were induced after exposure to the three antibiotics. The toxic effects were most obvious after moxifloxacin (RVEC, ≥125 μg/mL; pericytes, ≥1000 μg/mL) at 24 h. Cefuroxime also reduced esterase activity and the membrane integrity of vascular cells but were less toxic than moxifloxacin. Kinetic cell viability testing showed that 500 μg/mL of moxifloxacin exposure induced significant decrease (29%) in the viability as early as 1 h. When the inflammatory effects of the antibiotics were examined, a significant induction of IL-8 was observed especially by RVECs after exposure to cefuroxime or vancomycin which was exacerbated by L-alanyl-γ-D-glutamyl-meso-diaminopimelic acid (Tri-DAP), a NOD1 ligand. Intravitreal injections in mice showed that cefuroxime and vancomycin caused retinal and vascular toxicity extending to the inner nuclear layers. Collectively, moxifloxacin causes immediate damage to retinal vascular cells in vitro, while cefuroxime and vancomycin induced significant inflammatory effects on vascular endothelial cells and caused retinal toxicity. Surgeons need to be cautious of the toxicity when antibiotics are used prophylactically especially by intravitreal administration.

show abstract

Section: Discussionmentioning

confidence: 99%

Toxicities of and inflammatory responses to moxifloxacin, cefuroxime, and vancomycin on retinal vascular cells

Miyake

Miyazaki

Shimizu

et al. 2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Yoeruek et al 31 reported dose-dependent cefuroxime toxicity in cultured human corneal endothelial cells with toxicity observed in doses above 2.75 mg/mL. In another in vitro study, Haruki et al 32 found corneal damage at 24 hours in cefuroxime concentrations over 0.5mg/mL but not at 6 hours even at higher concentrations. Considering that aqueous humour is replenished at the rate of 1% per minute in a normal eye, for the standard dose of 1mg of ICC, the concentration of cefuroxime in the AC would decrease to 28% (0.76mg/mL) within 1 hour.…”

Section: Discussionmentioning

confidence: 99%

High dose cefuroxime causing retinal toxicity in a patient undergoing trabeculectomy

Wong

Steeples

et al. 2022

American Journal of Ophthalmology Case Reports

View full text Add to dashboard Cite

“…450 Several studies support the safety of intracameral moxifloxacin injection for endophthalmitis prophylaxis; 441,446,451 however, there is some evidence that higher concentrations may have a deleterious effect on the corneal endothelium. [452][453][454] Intracameral cefuroxime is available as a government-approved product in Europe, and moxifloxacin is commercially available in India. Because there is no FDA-approved product in the United States for endophthalmitis prophylaxis, moxifloxacin must be compounded for intracameral injection.…”

Section: P23mentioning

confidence: 99%

Cataract in the Adult Eye Preferred Practice Pattern®

et al. 2022

View full text Add to dashboard Cite

show abstract

Comparison of toxicities of moxifloxacin, cefuroxime, and levofloxacin to corneal endothelial cells in vitro

Abstract: Dr. Inoue is a medical advisor to Alcon Japan Ltd. No author has a financial or proprietary interest in any material or method mentioned.

Cited by 22 publications

References 25 publications

Toxicities of and inflammatory responses to moxifloxacin, cefuroxime, and vancomycin on retinal vascular cells

Toxicities of and inflammatory responses to moxifloxacin, cefuroxime, and vancomycin on retinal vascular cells

High dose cefuroxime causing retinal toxicity in a patient undergoing trabeculectomy

Cataract in the Adult Eye Preferred Practice Pattern®

Contact Info

Product

Resources

About