Comparison of total and intact aminoterminal propeptide of type I procollagen assays in patients with breast cancer with or without bone metastases

Marin, Lauri; Koivula, Minna; Jukkola-Vuorinen, Arja; Leino, Aila; Risteli, Juha

doi:10.1258/acb.2011.011040

Cited by 30 publications

(16 citation statements)

References 15 publications

(38 reference statements)

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“…The intact PINP assay automated is available on IDS-iSYS (Immunodiagnostic Systems); a radioimmunoassay for intact PINP is also available (UniQ PINP RIA Orion Diagnostica, Epsoo, Finland). Whilst results from current assays for PINP are comparable to the results from 'healthy' osteoporosis subjects with normal renal function, there is a potential for heterogeneity in patients with renal failure or metastatic bone disease, as it is claimed that the monomeric fragments may accumulate in these conditions [35, 36]. Pre-analytical advantages of PINP include a low diurnal and intra-individual variability, and stability at room temperature.…”

Section: Laboratory Investigations In Osteoporosismentioning

confidence: 99%

Current Recommendations for Laboratory Testing and Use of Bone Turnover Markers in Management of Osteoporosis

2012

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Osteoporosis is a major health problem worldwide, and is projected to increase exponentially due to the aging of the population. The absolute fracture risk in individual subjects is calculated by the use of algorithms which include bone mineral density (BMD), age, gender, history of prior fracture and other risk factors. This review describes the laboratory investigations into osteoporosis which include serum calcium, phosphate, creatinine, alkaline phosphatase and 25-hydroxyvitamin D and, additionally in men, testosterone. Parathyroid hormone (PTH) is measured in patients with abnormal serum calcium to determine its cause. Other laboratory investigations such as thyroid function testing, screening for multiple myeloma, and screening for Cushing's syndrome, are performed if indicated. Measurement of bone turnover markers (BTMs) is currently not included in algorithms for fracture risk calculations due to the lack of data. However, BTMs may be useful for monitoring osteoporosis treatment. Further studies of the reference BTMs serum carboxy terminal telopeptide of collagen type I (s-CTX) and serum procollagen type I N-terminal propeptide (s-PINP) in fracture risk prediction and in monitoring various treatments for osteoporosis may help expedite their inclusion in routine clinical practice.

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Section: Laboratory Investigations In Osteoporosismentioning

confidence: 99%

Current Recommendations for Laboratory Testing and Use of Bone Turnover Markers in Management of Osteoporosis

2012

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“…The retention of some of these molecules in blood of patients with renal impairment can make the interpretation of some total PINP results difficult [5]. It has been suggested that the monomeric form detected by the total PINP assay may be a breakdown product of collagen, and in breast cancer patients, for example, PINP has been shown to be higher by the Roche E170 total PINP assay as compared with the IDS iSYS method [13]. Therefore, whether the detection of such PINP molecules by total PINP assays may offer some additional information in specific groups of patients, e.g.…”

Section: Discussionmentioning

confidence: 99%

IDS iSYS automated intact procollagen-1-N-terminus pro-peptide assay: method evaluation and reference intervals in adults and children

Morovat

Catchpole

Meurisse

et al. 2013

Clinical Chemistry and Laboratory Medicine (CCLM)

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Background: We carried out a technical evaluation of the Immunodiagnostic Systems (IDS) automated intact procollagen-I N-terminus propeptide (PINP) assay on the iSYS platform, and established reference intervals for PINP in both adults and children. Methods: Assay imprecision, recovery and interference were studied. Serum and plasma values were compared, and PINP stability was assessed. Using 828 specimens, IDS iSYS intact PINP and Roche E170 total PINP values were compared. Specimens from 597 adults and 485 children and adolescents were used to establish reference intervals for intact PINP. Results: The method demonstrated good recovery and acceptable imprecision. The assay was unaffected by icterus and lipaemia, but haemolysis decreased measured PINP. Serum and plasma values were comparable. There was a non-linear relation between IDS intact and Roche total PINP values. Pre-and post-menopausal women had comparable PINP values, but there was a difference between women of different age groups. Serum PINP in men showed a decline in young age up to 45 years, but remained steady thereafter. Separate reference intervals were established for four age groups in women and for two age groups in men. Data for children were partitioned into four-year age groups, and these showed PINP to be high with no major gender differences until 12 years of age. Thereafter, values in females decreased in 13-16 years age groups and further in 17-20 years age groups, whereas PINP increased in boys of 13-16 years of age with a subsequent decline at 17-20 years. Conclusions: The IDS iSYS PINP intact assay appears to be reliable. We have established gender-and age-related reference intervals for children and adults based on a relatively large healthy North European population.

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“…Determinations of the intact, trimeric PINP and the total PINP in the sum of intact and monomeric PINP can, in the meantime, be performed on laboratory analyzers (Table 1). With a few exceptions, such as patients with kidney insufficiency due to a decreased clearance of the monomeric PINP form [39], the results of total PINP and intact PINP are well comparable [37,40]. However, differences under various clinical conditions have to be considered [35].…”

Section: Propeptides Of Type I Procollagenmentioning

confidence: 99%

“…Corresponding to their origin, the propeptides are called C-terminal or N-terminal propeptides of type I procollagen (PICP; PINP). PINP occurs in the serum in two forms: as intact, trimeric peptide corresponding to the native separation product of procollagen during the synthesis of type I collagen and as monomeric peptide which is rather a degradation product of procollagen [35][36][37]. In contrast to this view, there has been the assumption that the released intact PINP is rapidly degraded to the monomeric form when it is released into circulation and would make it necessary to determine both forms [38].…”

Section: Propeptides Of Type I Procollagenmentioning

confidence: 99%

Bone turnover markers in serum and urine as diagnostic, prognostic and monitoring biomarkers of bone metastasis

Jung

Lein

2014

Biochimica et Biophysica Acta (BBA) - Reviews on Cancer

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Comparison of total and intact aminoterminal propeptide of type I procollagen assays in patients with breast cancer with or without bone metastases

Abstract: Intact and total PINP assays give similar results in many conditions, but there are differences, for example in breast carcinoma, which should be recognized.

Cited by 30 publications

References 15 publications

Current Recommendations for Laboratory Testing and Use of Bone Turnover Markers in Management of Osteoporosis

Current Recommendations for Laboratory Testing and Use of Bone Turnover Markers in Management of Osteoporosis

IDS iSYS automated intact procollagen-1-N-terminus pro-peptide assay: method evaluation and reference intervals in adults and children

Bone turnover markers in serum and urine as diagnostic, prognostic and monitoring biomarkers of bone metastasis

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