Comparison of three techniques for the serological diagnosis of Neospora caninum in the dog and their use for epidemiological studies

Lasri, Sâadia; Meerschman, F. De; Rettigner, C.; Focant, Charles; Losson, Bertrand

doi:10.1016/j.vetpar.2004.05.025

Cited by 19 publications

(17 citation statements)

References 26 publications

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“…The lowest seroprevalences were found in groups 2 and 3 (police squad and kennels) whereas the highest seroprevalences were found in stray and farm dogs. This is in agreement with several studies indicating that seroprevalences are higher in farm dogs or pounds dogs than in urban dogs [9,10,[22][23][24][25][26][27][28][29][30][31][32]. In breeder (urban) dogs the seroprevalence was 12.08% which is close to the data available in similar urban dog populations [31,41].…”

Section: Discussionsupporting

confidence: 92%

“…In breeder (urban) dogs the seroprevalence was 12.08% which is close to the data available in similar urban dog populations [31,41]. In farm dogs the seroprevalence reached 46.24%; a similar high seroprevalence in this type of dogs was also noticed by Lasri et al [31] in Belgium. This is probably linked with the availability of infective material such as aborted fetuses and placentas [42,43].…”

Section: Discussionsupporting

confidence: 87%

“…Some studies investigated the seroprevalence in different dog populations in North America [22], in South America [23][24][25][26], in Asia [10,27,28], Oceania [29] or Europe [6,[30][31][32].…”

Section: Introductionmentioning

confidence: 99%

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First epidemiological study on exposure to Neospora caninum in different canine populations in the Algiers District (Algeria)

Ghalmi

China

Kaidi

et al. 2009

Parasitology International

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Neospora caninum is an important cause of abortion in cattle worldwide. Dogs act as final hosts shedding oocysts in the environment. They can also harbour the extraintestinal stage of the parasite and this may be associated with a fairly rare neuromuscular condition. The sera of 781 dogs from the Algiers District were screened by IFAT for the presence of anti-N. caninum antibodies. These dogs were distributed into four populations: local stray dogs, police dogs, dogs from breeding kennels and farm dogs. The overall seroprevalence was 21.90%. Significant differences were observed between the different populations, the highest prevalence being observed in farm (44.44%) and stray dogs (22.55%). Additionally, the highest titres were observed in farm dogs. Among studied epidemiological parameters, breed, dog origin, season and vaccination status were significantly associated with IFAT results. Additionally, a recently described real time PCR was used on the blood of 100 pound dogs and the results were compared with the serological data. A higher proportion of dogs was found to be positive by PCR when compared to the IFAT results. There was only a fairly low agreement between PCR and IFAT results which suggests that these techniques measured different aspects of the host-parasite relationship. This study indicates that the level of exposure of the canine population of Algiers area to N. caninum is very high. This would indicate a potentially high risk for N. caninum induced abortion in cattle in this region and in Algeria.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 87%

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First epidemiological study on exposure to Neospora caninum in different canine populations in the Algiers District (Algeria)

Ghalmi

China

Kaidi

et al. 2009

Parasitology International

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“…Seropositivity percentages were compared by the Chi-square ( 2 ) test or Fisher exact test, when appropriate. The agreement between IFAT and ELISA for the detection of antibodies to T. gondii and N. caninum was analyzed by calculating the proportion of observed agreement (P o ), the proportion of positive (P pos ) and negative (P neg ) agreeement, and the Kappa (Ä) coefficient as previously reported (Lasri et al, 2004;Silva et al, 2007). Values of P < 0.05 were considered statistically significant.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Toxoplasma gondii and Neospora caninum infections in sheep from Uberlândia, Minas Gerais State, Brazil, by different serological methods

Rossi

Cabral

Ribeiro

et al. 2011

Veterinary Parasitology

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Toxoplasmosis and neosporosis have been recognized as economically important diseases with considerable impact on the livestock industry. Considering the scarce information on the occurrence of Toxoplasma gondii and Neospora caninum infections in sheep from Uberlândia, Minas Gerais State, Brazil, this study aimed to investigate the frequency of antibodies against these parasites in sheep sera from this region by using different serological methods. A total of 155 sheep serum samples were analyzed by the indirect fluorescence antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) for the detection of IgG against T. gondii and N. caninum. Seroreactivity by IFAT showed 80% of samples with titers between 512 and 2048 for T. gondii (cutoff ≥ 64) and 78% presenting titers between 50 and 200 for N. caninum (cutoff ≥ 50). Seroreactivity by ELISA showed 75% of samples with ELISA index (EI) between 2.0 and 3.0 for T. gondii (cutoff ≥ 1.3) and 54% presenting EI between 1.3 and 2.0 for N. caninum (cut off ≥ 1.3). Discordant results by both tests were analyzed by immunoblot, resulting in a total seropositivity of 61% for T. gondii and 23% for N. caninum, with 41% to T. gondii only, 3% to N. caninum only, and 20% to both parasites. There was a significant positive association between seropositivity to T. gondii and age over one year (P<0.001), but such association was not found for N. caninum infection. In conclusion, as T. gondii and N. caninum infections are simultaneously present in sheep flocks of this region, it should be emphasized the importance to carry out a regular monitoring of Toxoplasma infection due to its high prevalence, its zoonotic potential and induction of reproductive disorders leading to economic losses. For neosporosis, sheep farmers should be instructed about the presence of the parasite in the flock, its risk factors and potential abortifacient role in sheep. Differential flock management could be valuable tool to establish the association of serological positivity and reproductive disease induced by N. caninum in sheep.

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“…A maior parte destes trabalhos utiliza testes de Imunofluorescência indireta (RIFI) ou ensaio por imunoabsorbância ligado a enzima (ELISA) para detectar anticorpos contra Neospora spp., sendo o segundo método, o de preferência para estudos epidemiológicos com grande número de amostras (Lasri et al 2004). O emprego do teste de aglutinação, também já foi realizado para pesquisa de anticorpos contra N. caninum em soro sanguíneo de equinos .…”

Section: Discussionunclassified

Verificação da transmissão vertical de Neospora spp. em equinos

et al. 2015

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O gênero protozoário Neospora é reconhecido como causador de desordens reprodutivas e abortos em bovinos. Entre os equinos pouco se sabe sobre os efeitos da infecção por estes protozoários. Atualmente é admitido que os efeitos da infecção por Neospora hughesi em equinos possam ocorrer no sistema nervoso central e, os efeitos provocados pela infecção por Neospora caninum recaiam sobre o sistema reprodutor de éguas. O presente trabalho verificou a presença de imunoglobulinas da classe G no soro sanguíneo de uma população de éguas de cria e, em seus respectivos potros antes da ingestão do colostro. Para execução deste trabalho foi empregada técnica de imunofluorescência indireta (RIFI), utilizando como antígeno taquizoítos de Neospora caninum, a diluição inicial dos soros das éguas foi de 1:50 e a diluição do soro dos potros empregada foi de 1:16. Foram assistidos 78 partos e todos os potros tiveram seu soro sanguíneo coletado imediatamente após o nascimento. A pesquisa de anticorpos contra Neospora spp. apontou que 50 (64%) éguas e 32 (41%) potros foram positivos. Das 50 éguas que apresentaram anticorpos contra Neospora spp. 24 geraram potros positivos. Entre as 28 éguas que não reagiram, oito deram a luz a potros positivos. De posse dos resultados encontrados podemos concluir que ocorreu a transmissão vertical de Neospora spp. nos equinos pesquisados.

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Comparison of three techniques for the serological diagnosis of Neospora caninum in the dog and their use for epidemiological studies

Cited by 19 publications

References 26 publications

First epidemiological study on exposure to Neospora caninum in different canine populations in the Algiers District (Algeria)

First epidemiological study on exposure to Neospora caninum in different canine populations in the Algiers District (Algeria)

Evaluation of Toxoplasma gondii and Neospora caninum infections in sheep from Uberlândia, Minas Gerais State, Brazil, by different serological methods

Verificação da transmissão vertical de Neospora spp. em equinos

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