Comparison of three sequential extraction procedures to describe metal fractionation in anaerobic granular sludges

Hullebusch, Eric D. van; Sudarno, S.; Zandvoort, Marcel H.; Lens, Piet N.L.

doi:10.1016/j.talanta.2004.07.024

Cited by 124 publications

(79 citation statements)

References 28 publications

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“…The formation of these precipitates under the applied conditions was also predicted by calculations with Visual MINTEQ (data not shown). Those precipitates are known to be present in granular sludge in significant amounts and have to be taken into account for interpretation of data when the solid-state speciation is investigated in granular sludge [42].…”

Section: Role Of Speciation In Cobalt Toxicitymentioning

confidence: 99%

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Cobalt toxicity in anaerobic granular sludge: influence of chemical speciation

Bartacek

Fermoso

Baldó-Urrutia

et al. 2008

J Ind Microbiol Biotechnol

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The influence of cobalt speciation on the toxicity of cobalt to methylotrophic methanogenesis in anaerobic granular sludge was investigated. The cobalt speciation was studied with three different media that contained varying concentrations of complexing ligands [carbonates, phosphates and ethylenediaminetetraacetic acid (EDTA)]. Three fractions (nominal added, dissolved and free) of cobalt were determined in the liquid media and were correlated with data from batch toxicity experiments. The average concentration of cobalt that was required for 50% inhibition of methanogenic activity (IC 50 ) for free Co 2? in the three sets of measurements was 13 lmol/L with a standard deviation of 22% and a similarity of 72% between the data obtained in the three different media for the range of cobalt concentrations investigated. The standard deviation of the IC 50 for the other two fractions was much higher, i.e. 85 and 144% for the added cobalt and dissolved cobalt, respectively, and the similarity was almost 0% for both fractions. Complexation (and precipitation) with EDTA, phosphates and carbonates was shown to decrease the toxicity of cobalt on methylotrophic methanogenesis. The free cobalt concentration is proposed to be the key parameter to correlate with cobalt toxicity. Thus, the toxicity of cobalt to granular sludge can be estimated based on the equilibrium-free cobalt concentration.

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Section: Role Of Speciation In Cobalt Toxicitymentioning

confidence: 99%

“…Thus, cobalt adsorption plays a key role in the cobalt distribution in this system. However, it is almost impossible to distinguish between precipitation and adsorption in the granular sludge matrix [42]. Therefore, precipitation of part of the cobalt added (e.g.…”

Section: Role Of Speciation In Cobalt Toxicitymentioning

confidence: 99%

Cobalt toxicity in anaerobic granular sludge: influence of chemical speciation

Bartacek

Fermoso

Baldó-Urrutia

et al. 2008

J Ind Microbiol Biotechnol

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“…The sequential extraction methods are widely used for analysis of trace metal fractionations in sludge from biogas reactors e.g. [16][17][18][19], but possible changes in the S chemistry during sequential extractions, and how they may affect operationally defined trace metal fractions, have not been investigated previously for sludge samples from biogas reactors. In a parallel study, we observed that Co (but not Ni) was encountered in the residual fraction (after extraction of organic/sulfide bonded trace metals) of a biogas sludge together with Fe (Gustavsson et al, unpublished).…”

Section: Introductionmentioning

confidence: 99%

“…The feasibility of three different sequential extraction procedures used for analysis of trace metal fractionations in anaerobic granular sludge were compared by Hullebusch et al [17]. They suggested that a modified version of the method developed by Tessier et al [1] as described by Osuna et al [4], has a better reproducibility and reagent selectivity compared to other sequential extraction methods.…”

Section: Introductionmentioning

confidence: 99%

Sulfur K-edge XANES and acid volatile sulfide analyses of changes in chemical speciation of S and Fe during sequential extraction of trace metals in anoxic sludge from biogas reactors

Yekta

Gustavsson

Svensson

et al. 2012

Talanta

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The effect of sequential extraction of trace metals on sulfur (S) speciation in anoxic sludge samples from two lab-scale biogas reactors augmented with Fe was investigated. Analyses of sulfur K-edge X-ray absorption near edge structure (S XANES) spectroscopy and acid volatile sulfide (AVS) were conducted on the residues from each step of the sequential extraction. The S speciation in sludge samples after AVS analysis was also determined by S XANES. Sulfur was mainly present as FeS (~60% of total S) and reduced organic S (~30% of total S), such as organic sulfide and thiol groups, in the anoxic solid phase. Sulfur XANES and AVS analyses showed that during first step of the extraction procedure (the removal of exchangeable cations), a part of the FeS fraction corresponding to 20% of total S was transformed to zero-valent S, whereas Fe was not released into the solution during this transformation. After the last extraction step (organic/sulfide fraction) a secondary Fe phase was formed. The change in chemical speciation of S and Fe occurring during sequential extraction procedure suggests indirect effects on trace metals associated to the FeS fraction 2 that may lead to incorrect results. Furthermore, by S XANES it was verified that the AVS analysis effectively removed the FeS fraction. The present results identified critical limitations for the application of sequential extraction for trace metal speciation analysis outside the framework for which the methods were developed.

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“…Direct methods are generally insufficient sensitivity for metal speciation analysis or require very specialized equipments such as synchrotron X-ray absorption spectroscopy (Kersten, 2002;Pueyo et al, 2003). Despite some disadvantages such as non-selectivity of extractants and redistribution of heavy metals among phases during extraction, sequential extraction methods are still commonly used to determine metal fractionation in soil to provide an understanding of mobility and bioavailability of metals (Tessier et al, 1979;Hullebusch et al, 2005). The Community Bureau of Reference (BCR) method has been widely used in metal fractionation for both soil and sediment (Quevauiller et al, 1993;Fernández et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Aging effect on the mobility and bioavailability of copper in soil

Zhang

Qin

et al. 2009

Journal of Environmental Sciences

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Aging effect on the mobility and bioavailability of copper (Cu) was investigated using a spiked soil with different incubation periods from 3 to 56 d. Wheat was planted and earthworms were cultured separately in the incubated soils. The mobility of Cu in soil was evaluated by a chemical fractionation scheme and the toxicity and bioavailability were assessed by measuring the biomass and Cu concentration in tissues. Results showed that aging had a significant effect on Cu fraction distribution, of which Cu tended to incorporate from the exchangeable into more stable fractions such as the reducible and oxidisable fractions. However, aging had little effect on Cu bioavailability to wheat and earthworm. Comparing the soil being incubated for 3 d and 56 d, Cu concentration in wheat roots decreased from 14.5 to 12.8 mg/kg, and no significant changes in Cu concentration were observed in both wheat shoots and earthworms. The Cu concentration was around 2.0 and 50 mg/kg for wheat shoots and earthworms, respectively, irrespective of soil incubation time. The CaCl 2 -extractable Cu had a linear relationship with Cu concentration in wheat roots (R 2 = 0.65, P < 0.05), but no linear relationship can be found for wheat shoots and earthworms. Biological control may be more crucial for Cu accumulation in organism than the changes in soil Cu fraction caused by aging.

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Comparison of three sequential extraction procedures to describe metal fractionation in anaerobic granular sludges

Cited by 124 publications

References 28 publications

Cobalt toxicity in anaerobic granular sludge: influence of chemical speciation

Cobalt toxicity in anaerobic granular sludge: influence of chemical speciation

Sulfur K-edge XANES and acid volatile sulfide analyses of changes in chemical speciation of S and Fe during sequential extraction of trace metals in anoxic sludge from biogas reactors

Aging effect on the mobility and bioavailability of copper in soil

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