Comparison of three procedures for the rapid identification of bacteraemia-causing microorganisms. Evaluation of their effectiveness and applicability to microbiology laboratories

Martín-Pujol, Oriol; Tosco-Núñez, Tomás; Miguel-Martínez, Isabel de

doi:10.1016/j.eimce.2018.06.009

Cited by 2 publications

(4 citation statements)

References 15 publications

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“…Different lysis reagents may be employed, in particular Saponin, Sodium dodecyl sulfate (SDS), Ammonium chloride or Triton X-100 with sensibilities ranging from 53 to 86% [34,53,54]. Additional extraction steps using either ethanol, formic acid or acetonitrile have also been described improving sensibilities from 49% to 88.9% [40,41,[55][56][57][58][59]. Time to results relies mainly on the number of extraction and centrifugation steps in each protocol and therefore varies between 10 to 45 min which limits the use of many of them in routine diagnostic procedures (Table 1) [34,40,41,[53][54][55][56][57][58][59].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Rapid Sepsityper® protocol and specific MBT-Sepsityper module (Bruker Daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS

Ponderand

Pavèse

Maubon

et al. 2020

Ann Clin Microbiol Antimicrob

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During bloodstream infections, rapid adaptation of empirical treatment according to the microorganism identified is essential to decrease mortality. The aim of the present study was to assess the microbiological performances of a new rapid version of the Sepsityper® kit (Bruker Daltonics) allowing identification of bacteria and yeast by MALDI-TOF mass spectrometry directly from positive blood cultures in 10 min and of the specific MBT-Sepsityper module for spectra analysis, designed to increase identification performance. Identification rates were determined prospectively on 350 bacterial and 29 fungal positive blood cultures, and compared to conventional diagnostic method. Our rapid diagnosis strategy (Rapid Sepsityper® protocol: one spot with and one without formic acid extraction step) combined to MBT-Sepsityper module provided 65.4%, 78.9% and 62% reliable identification to the species level of monomicrobial positive blood cultures growing respectively Gram-positive, Gram-negative bacteria or yeast. Importantly, identification rates of Gram-positive bacteria were higher in anaerobic than in aerobic bottles (77.8% vs 22.2%; p = 0.004), if formic acid extraction step was performed (60.8% vs 39.2%; p = 1.8e−6) and if specific MBT-Sepsityper module was used (76.2% vs 61.9%, p = 0.041) while no significant differences were observed for Gram-negative bacteria. For yeasts identification, formic acid extraction step improved rapid identification rate by 37.9% while the specific MBT-Sepsityper module increased overall performances by 38%, providing up to 89.7% reliable identification if associated with the standard Sepsityper® protocol. These performances, associated with a reduce turnaround time, may help to implement a rapid identification strategy of bloodstream infections in the routine workflow of microbiology laboratories.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Rapid Sepsityper® protocol and specific MBT-Sepsityper module (Bruker Daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS

Ponderand

Pavèse

Maubon

et al. 2020

Ann Clin Microbiol Antimicrob

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“…The CE IVD labelled MBT Sepsityper IVD Kit allows the micro-organism ID directly from the positive BCs by MALDI-TOF MS within 15-20 min [20,21], and it was proved to exhibit a good efficacy [21][22][23][24][25][26][27][28][29][30][31]. It relies on the lysis of blood cells, a nondisruptive enrichment of micro-organisms, followed by a centrifugation and a washing step, to obtain a microbial pellet.…”

Section: Discussionmentioning

confidence: 99%

“…The use of MALDI-TOF MS for reducing the time to report has been widely investigated, and a plethora of methods and protocols have been described [10–15, 21, 33]. The CE IVD labelled MBT Sepsityper IVD Kit allows the micro-organism ID directly from the positive BCs by MALDI-TOF MS within 15–20 min [20, 21], and it was proved to exhibit a good efficacy [21–31]. It relies on the lysis of blood cells, a nondisruptive enrichment of micro-organisms, followed by a centrifugation and a washing step, to obtain a microbial pellet.…”

Section: Discussionmentioning

confidence: 99%

“…The MBT Sepsityper IVD Kit (Bruker Daltonics GmbH and Co KG, Bremen, Germany) is a wide-spread sample preparation method, which proved to have a good efficacy [20][21][22][23], also in its simplified and shortened protocol version (Rapid Sepsityper) [24,25]. Many studies were performed to compare short subculturing to the Sepsityper, showing discordant results in terms of rate of correct ID [26][27][28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

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Identification of micro-organism from positive blood cultures: comparison of three different short culturing methods to the Rapid Sepsityper workflow

Pranada¹,

Cordovana

Meyer

et al. 2022

Journal of Medical Microbiology

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Sepsis is one of the leading causes of death worldwide. The rapid identification (ID) of the causative micro-organisms is crucial for the patients’ clinical outcome. MALDI-TOF MS has been widely investigated to speed up the time-to-report for ID from positive blood cultures, and many different procedures and protocols were developed, all of them attributable either to the direct separation of microbial cells from the blood cells, or to a short subculture approach. In this study, the Rapid Sepsityper workflow (MBT Sepsityper IVD Kit, Bruker Daltonics GmbH and Co. KG, Bremen, Germany) was compared to three different short subculturing methods, established into the routine practice of three different clinical microbiology laboratories. A total of N=503 routine samples were included in this study and tested in parallel with the two approaches. Results of the rapid procedures were finally compared to routine proceedings with Gram-staining and overnight subculture. Among monomicrobial samples, the Rapid Sepsityper workflow enabled overall the correct identification of 388/443 (87.6 %) micro-organisms, while the short subculturing methods of 267/435 (61.8 %). Except for the performance with Streptococcus pneumoniae , in each one of the three sites the Rapid Sepsityper workflow proved to be superior to the short subculture method, regardless of the protocol applied, and it delivered a result from 1 to 5 h earlier.

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Comparison of three procedures for the rapid identification of bacteraemia-causing microorganisms. Evaluation of their effectiveness and applicability to microbiology laboratories

Cited by 2 publications

References 15 publications

Evaluation of Rapid Sepsityper® protocol and specific MBT-Sepsityper module (Bruker Daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS

Evaluation of Rapid Sepsityper® protocol and specific MBT-Sepsityper module (Bruker Daltonics) for the rapid diagnosis of bacteremia and fungemia by MALDI-TOF-MS

Identification of micro-organism from positive blood cultures: comparison of three different short culturing methods to the Rapid Sepsityper workflow

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