Comparison of Three Molecular Subtyping Methods among O157 and Non-O157 Shiga Toxin-Producing &lt;i&gt;Escherichia coli&lt;/i&gt; Isolates from Japanese Cattle

Nakamura, Hiromi; Iguchi, Atsushi; Maehara, Tomofumi; Fujiwara, Kyohei; Fujiwara, Atsushi; Ogasawara, Jun

doi:10.7883/yoken.jjid.2017.297

Cited by 4 publications

(2 citation statements)

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“…Reports indicate a wide variety of serotypes and sequence types of STEC isolated in Iran, Japan, Argentina, Brazil, among others, from diverse sources. Many of these studies have used techniques such as MLST, traditional serotyping, and PFGE to study STEC isolates (Feng et al, 2014;Cadona et al, 2016;Ferdous et al, 2016;Jajarmi et al, 2017;Nakamura et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces

et al. 2021

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Shiga toxin-producing Escherichia coli (STEC) causes foodborne outbreaks that can lead to complications such as hemolytic uremic syndrome. Their main reservoir is cattle, and ground beef has been frequently associated with disease and outbreaks. In this study, we attempted to understand the genetic relationship among STEC isolated in Chile from different sources, their relationship to STEC from the rest of the world, and to identify molecular markers of Chilean STEC. We sequenced 62 STEC isolated in Chile using MiSeq Illumina. In silico typing was determined using tools of the Center Genomic Epidemiology, Denmark University (CGE/DTU). Genomes of our local STEC collection were compared with 113 STEC isolated worldwide through a core genome MLST (cgMLST) approach, and we also searched for distinct genes to be used as molecular markers of Chilean isolates. Genomes in our local collection were grouped based on serogroup and sequence type, and clusters were formed within local STEC. In the worldwide STEC analysis, Chilean STEC did not cluster with genomes of the rest of the world suggesting that they are not phylogenetically related to previously described STEC. The pangenome of our STEC collection was 11,650 genes, but we did not identify distinct molecular markers of local STEC. Our results showed that there may be local emerging STEC with unique features, nevertheless, no molecular markers were detected. Therefore, there might be elements such as a syntenic organization that might explain differential clustering detected between local and worldwide STEC.

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Section: Introductionmentioning

confidence: 99%

Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces

et al. 2021

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“…There are several methods for typing of E. coli from environmental samples including traditional and modern molecular methods [7][8][9][10][11][12][13]. With the advent of the molecular age, scientists changed their approach from phenotyping to genotyping methods [14,15].…”

Section: Introductionmentioning

confidence: 99%

Multilocus Variable Number of Tandem Repeat Analysis for molecular typing of uropathogenic Escherichia coli

Ranjbar¹,

Dehkordi²,

Mashhouri³

et al. 2020

Preprint

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Background: The aim of this study was genotyping of Uropathogenic Escherichia coli (UPEC) based on Variable Number of Tandem Repeats (VNTRs) sequences. Methods: E. coli strains isolated from urine samples were included in this study. Seven VNTR loci were subjected to Multilocus variable-number tandem repeat analysis (MLVA) based on PCR amplification. Then data was analyzed via online mlvaplus software and the information was displayed in the form of MST analysis. Results: A total of 100 E. coli strains were isolated and subjected to the study. MLVA was able to differentiate 56 different genotypes. Also, the technique could classify E. coli isolates in 5 clonal complexes. Based on UPGMA dendrograms, E. coli isolates were classified into 4 clusters (clusters A to D). The strains associated with Complex No. 1 appeared to be dominant pathogens of UPEC in Tehran's patients. The present study provides valuable insights into the genetic relationships of E. coli isolates recovered from clinical cases in a major hospital in Iran. Conclusions: The analysis of MLVA profiles using the MST algorithm showed the usefulness of the MLVA method in the classification of uropathogenic E. coli collected in different periods. We evaluated MLVA in a laboratory equipped with simple molecular equipment. Based on these results, it has been assumed that the E. coli strains were derived from a limited number of clones that have undergo a small genetic change during this period.

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Study of the transfer of Shiga toxin-producing Escherichia coli during the slaughter of cattle using molecular typing combined with epidemiologic data

Jiang,

Zhang,

Nychas

et al. 2024

Meat Science

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Comparison of Three Molecular Subtyping Methods among O157 and Non-O157 Shiga Toxin-Producing <i>Escherichia coli</i> Isolates from Japanese Cattle

Cited by 4 publications

References 40 publications

Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces

Whole-Genome Phylogenetic Analysis Reveals a Wide Diversity of Non-O157 STEC Isolated From Ground Beef and Cattle Feces

Multilocus Variable Number of Tandem Repeat Analysis for molecular typing of uropathogenic Escherichia coli

Study of the transfer of Shiga toxin-producing Escherichia coli during the slaughter of cattle using molecular typing combined with epidemiologic data

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