Comparison of Three Molecular Methods for the Detection and Speciation of Five Human Plasmodium Species

Lau, Yee Ling; Lai, Meng Yee; Anthony, Claudia; Chang, Phooi Yee; Palaeya, Vanitha; Fong, Mun Yik; Mahmud, Rohela

doi:10.4269/ajtmh.14-0309

Cited by 21 publications

(23 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Two subspecies of P. ovale (P. ovale curtisi and P. ovale wallikeri) are usually difficult to be distinguished by microscopy. Misidentification may also happen between different species [27]. All above may induce the unreasonable usage of antimalarial drugs and then hamper the parasite clearance and lead to the transmission of malaria, even antimalarial drug resistance.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018

Xie

Cheng

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Malaria remains a serious public health problem globally. Along with indigenous malaria elimination in China, imported malaria gradually became a major hazard. Well-timed and accurate diagnosis could support immediate therapeutic schedule, reveal the prevalence of imported malaria and avoid the disease transmission. Method Blood samples were collected in Wuhan, China from August 2011 to December 2018. All patients first accepted microscopy and RDT examination. Subsequently, each of the positive or suspected positive cases was engaged in total four human Plasmodium species amplication by using 18S rRNA based nested PCR and Taqman probe based real-time PCR. Then performance of microscopy and two molecular diagnosis methods were analyzed. Importation origin was traced by country and prevalence of Plasmodium species was revealed by year. Results Total 296 blood samples containing 288 microscopy and RDT positive, 7 RDT P. falciparum positive and 1 suspected cases were collected and reanalyzed. After two molecular methods and sequencing detection, 291 cases including 245 P. falciparum , 15 P. vivax , 20 P. ovale , 6 P. malariae and 5 mixed infection (3 P. falciparum + P. ovale , 2 P. vivax + P. ovale ) were confirmed. These patients returned from Africa (95.53%) and Asia (4.47%). Although the prevalence displayed a small-scale fluctuation, the overall trend of the imported cases increased yearly. Conclusions Results emphasized the necessity of combined utilization of the four tools for malaria diagnosis in clinic and field survey around potential risk regions worldwide including Wuhan.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018

Xie

Cheng

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…The two subspecies of P. ovale (P. ovale curtisi and P. ovale wallikeri) are di cult to be distinguished by microscopy. Misidenti cation may also happen between different species [30]. All of the above may induce the unreasonable usage of antimalarial drugs, hamper parasite clearance and lead to the transmission of malaria, and even antimalarial drug resistance.…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018

Xie

Cheng

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Malaria remains a serious public health problem globally. As the elimination of indigenous malaria continues in China, imported malaria has gradually become a major health hazard. Well-timed and accurate diagnoses could support the timely implementation of therapeutic schedules, reveal the prevalence of imported malaria and avoid transmission of the disease. Methods Blood samples were collected in Wuhan, China, from August 2011 to December 2018. All patients accepted microscopy and rapid diagnosis test (RDT) examinations. Subsequently, each of the positive or suspected positive cases was tested for four human-infectious Plasmodium species by using 18S rRNA-based nested PCR and Taqman probe-based real-time PCR. The results of the microscopy and the two molecular diagnostic methods were analysed. Importation origins were traced by country, and the prevalence of Plasmodium species was analysed by year. Results A total of 296 blood samples, including 288 that were microscopy and RDT positive, 7 RDT and Plasmodium falciparum positive, and 1 suspected case, were collected and reanalysed. After application of the two molecular methods and sequencing, 291 cases including 245 P. falciparum, 15 Plasmodium vivax, 20 Plasmodium ovale, 6 Plasmodium malariae and 5 mixed infections (3 P. falciparum + P. ovale, 2 P. vivax + P. ovale) were confirmed. These patients had returned from Africa (95.53%) and Asia (4.47%). Although the prevalence displayed a small-scale fluctuation, the overall trend of the imported cases increased yearly. Conclusions These results emphasize the necessity of combined utilization of the four tools for malaria diagnosis in clinic and in field surveys of potential risk regions worldwide including Wuhan.

show abstract

“…By using P. knowlesi β-tubulin gene as the target gene, Iseki et al 12 managed to detect P. knowlesi down to 200 copies of DNA only. However, using 18S rRNA as the target gene, the sensitivity of LAMP was improved and managed to detect P. knowlesi DNA down to 10 copies by Lau et al 13 Real-time PCR was another approach used to detect P. knowlesi because of its low detection limit ability. Divis et al 14 reported that the analytical sensitivity for amplification of P. knowlesi-specific assay was 10 copies/μL using a TaqMan real-time PCR assay.…”

mentioning

confidence: 99%

Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi

Lai

Ooi

Lau

2018

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

The aim of this study was to develop a recombinase polymerase amplification (RPA) combined with a lateral flow (LF) strip method for specific diagnosis of . With incubation at 37°C, the gene of was successfully amplified within 12 minutes. By adding a specifically designed probe to the reaction solution, the amplified RPA product can be visualized on a LF strip. The RPA assay exhibited high sensitivity with limits of detection down to 10 parasites/μL of. Nonetheless, it was demonstrated that all ( = 41) and other sp. ( = 25) were positive while negative samples ( = 8) were negative. Therefore, a combination of RPA and LF strip detection is a highly promising approach with the potential to be suitable for use in resource-limited settings.

show abstract

Comparison of Three Molecular Methods for the Detection and Speciation of Five Human Plasmodium Species

Cited by 21 publications

References 25 publications

Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018

Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018

Molecular epidemiological surveillance of Africa and Asia imported malaria in Wuhan, Central China: comparison of diagnostic tools during 2011–2018

Recombinase Polymerase Amplification Combined with a Lateral Flow Strip for the Detection of Plasmodium knowlesi

Contact Info

Product

Resources

About