Comparison of three methods for concentration of rotavirus from artificially spiked shellfish samples

Mohan, Vysakh; Rawat, Shashi; Lokesh, K.; Mohan, Hari; Reddy, D. Avinash; Kumar, Ashok; Bhilegaonkar, K.N.

doi:10.14202/vetworld.2014.463-466

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…The recovery efficiency, determined on the process control mengovirus, was >1% for all samples; thus the results are considered valid according to ISO 15216-2:2013. Of the positive shellfish, 16 were positive by both extraction methods used, while the remaining were positive by either one or the other (13 with proteinase K and 10 with glycine), confirming the results obtained in previous investigations (La Mohan et al, 2014), and thus indicating that the positives would have been underestimated if a single method had been used. A Chi-square test was used to examine their association.…”

Section: Resultssupporting

confidence: 85%

Molecular characterization of human enteric viruses in food, water samples, and surface swabs in Sicily

Purpari

Macaluso

Bella

et al. 2019

International Journal of Infectious Diseases

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Objectives: Enteric viruses are responsible for foodborne and waterborne infections affecting a large number of people. Data on food and water viral contamination in the south of Italy (Sicily) are scarce and fragmentary. The aim of this study was to evaluate the presence of viral contamination in food, water samples, and surface swabs collected in Sicily Methods: The survey was conducted on 108 shellfish, 23 water samples (seawater, pipe water, and torrent water), 52 vegetables, one peach and 17 berries, 11 gastronomic preparations containing fish products and/or raw vegetables, and 28 surface swabs. Hepatitis A virus (HAV), genogroup GI, GII, and GIV norovirus (NoV), enterovirus (EV), rotavirus (RoV), hepatitis E virus (HEV), adenovirus (AdV), and bocavirus (BoV) were detected by nested (RT) PCR, real-time PCR, and sequence analysis. Results: The most frequently detected viruses in shellfish were HAV (13%), NoV (18.5%), and EV (7.4%). Bocavirus was found in 3.7%, HEV in 0.9%, and AdV in 1.9% of the molluscs. Of the 23 water samples, 21.7% were positive for GII NoV and 4.3% for RoV and HEV genotype 3. Of the 70 vegetable samples, 2.9% were positive for NoV GI (GI.5 and GI.6), 2.9% for EV, and 1.4% for HEV. In the gastronomic preparations, only one EV (9%) was detected. No enteric viruses were detected in the berries, fruit, or swabs analyzed. Conclusions: Molecular surveillance of water and food samples clearly demonstrated that human pathogenic viruses are widely found in aquatic environments and on vegetables, and confirmed the role of vegetables and bivalve molluscs as the main reservoirs.

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Section: Resultssupporting

confidence: 85%

Molecular characterization of human enteric viruses in food, water samples, and surface swabs in Sicily

Purpari

Macaluso

Bella

et al. 2019

International Journal of Infectious Diseases

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Development of a new concentration method for Hepatitis A virus detection (ISO 15216–2:2019) in Manila clams (Ruditapes philippinarum)

Dian-Fu¹,

Cao²,

Tian³

et al. 2022

LWT

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Hydro-epidemiological aspects of infectious agent indication in mollusks (review)

Blokhin¹,

Toropova²,

Захарова³

et al. 2019

Аграрная наука Евро-Северо-Востока

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The article presents the rationale for the use of hydro-epidemiology in assessing the risk of spreading water-related in-fectious diseases of animals. The authors distinguish six hydro-epidemiological groups of diseases: 1) water is the medium of pathogen accumulation; 2) water is the medium for transmission of the infection pathogen without its biological accumula-tion; 3) the development cycle of the causative agent is directly related to water and aquatic organisms; 4) diseases transmitted by insects having water-related development cycle; 5) diseases transmitted by aquatic and near-water vertebrates; 6) diseases of reservoir inhabitants. At the same time, it is shown that the aquatic filter-feeding bivalves (mollusks) accumulate in them-selves various pathogenic microorganisms, which are the causative agents of infectious diseases of both man and animals. Thus, mollusks can serve as a test object for hydro-epidemiological study in assessing the risk of infectious diseases dissemi-nation in water area and near-water complex as well as in using them directly for food.

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Comparison of three methods for concentration of rotavirus from artificially spiked shellfish samples

Cited by 3 publications

References 16 publications

Molecular characterization of human enteric viruses in food, water samples, and surface swabs in Sicily

Molecular characterization of human enteric viruses in food, water samples, and surface swabs in Sicily

Development of a new concentration method for Hepatitis A virus detection (ISO 15216–2:2019) in Manila clams (Ruditapes philippinarum)

Hydro-epidemiological aspects of infectious agent indication in mollusks (review)

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