Comparison of three methods for the detection of Epstein-Barr virus in Hodgkin’s lymphoma in paraffin-embedded tissues

Qi, Zongli; Han, Xiaoyan; Hu, Jun; Wang, Guanghua; Gao, Jian‐Zhong; Wang, Xin; Liang, Dongxin

doi:10.3892/mmr.2012.1163

Cited by 30 publications

(21 citation statements)

References 16 publications

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“…EBER-ISH assay localizes EBV in biopsy tissue [1] • A biopsy assessed by EBER-ISH is needed to confirm each diagnosis and its relation to EBV We do IHC for EBV and recommend best practice of doing both in positive case so as to increase detection and association as the sensitivity and specificity in our study were 91% and 94%, respectively, which matched with the results demonstrated by Qi et al [5] which states to perform at least two methods to be performed for accurate results it also states that PCR is the most sensitive of the three methods, but it is unable to provide specific information with regard to the localization of EBVpositive cells which is possible in EBER and EBV by IHC.…”

Section: Discussionsupporting

confidence: 86%

Establishing etiopathogenesis in Epstein-Barr virus associated malignancies using chromogenic in situ hybridization

Patkar

2017

Int J Mol Immuno Oncol.

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<p>Background: Epstein–Barr virus is a human herpes virus ubiquitously infecting 90% of world population and causing 1% of tumors worldwide. The objectives were to establish etiopathogenesis in the Epstein-Barr virus (EBV) associated malignancies reported in our laboratory using the gold standard test, i.e., chromogenic in situ hybridization (CISH) for Epstein-Barr virus - encoded RNA (EBER). Aims and Objectives: We performed CISH on 244 cases of associated malignancies in the year 2013–2016, the decision was taken after H and E diagnosis and immunohistochemistry confirmation. Observation and Results: In the period of 2013–2016, 253 cases were evaluated by CISH for EBER. The age range in our study was 5–88 years with the mean age of 41.8 years, a slight male predominance was noted with male: female ratio of 1.5:1. CISH in 61 Classical Hodgkin lymphoma cases of 90cases were positive Conclusion: EBER ISH is the single best histochemical assay for defining EBV - related neoplasia. It is likely that emerging technologies such as gene expression profiling and proteomics will identify patterns of viral and human gene expression correlating with diagnosis, prognosis, and outcome in response to therapy.</p>

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Section: Discussionsupporting

confidence: 86%

Establishing etiopathogenesis in Epstein-Barr virus associated malignancies using chromogenic in situ hybridization

Patkar

2017

Int J Mol Immuno Oncol.

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“…Although EBV is a well-known risk factor for HL in endemic regions of the world, frequency of its association in our population has not been widely studied. There are various methods of detecting EBV in HL including immunohistochemistry (IHC), in situ hybridization (ISH), and polymerase chain reaction (PCR) [9]. EBV-infected tumor cells express a subset of EBV genes including latent membrane protein 1 (LMP1), LMP2a, EBV nuclear antigen 1 (EBNA1), EBV small nuclear RNA transcripts (EBER), and the BamHI A region transcripts [10–12].…”

Section: Introductionmentioning

confidence: 99%

Latent membrane protein 1 (LMP1) expression in Hodgkin lymphoma and its correlation with clinical and histologic parameters

et al. 2017

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BackgroundHodgkin lymphoma is one of the most prevalent lymphoproliferative disorders in Pakistan; however, no risk factors for this disease have yet to be established in our population. Epstein–Barr virus (EBV) is a well-known risk factor for Hodgkin lymphoma in endemic regions of the world; however, frequency of its association in our population has not been widely studied. Latent membrane protein 1 (LMP1) expression by immunohistochemistry (IHC) is a surrogate marker of EBV in Hodgkin lymphoma. Therefore, we aimed to evaluate the frequency of expression of LMP1 in cases of Hodgkin lymphoma at our institute and its correlation with other clinical and histologic parameters.MethodsThe study included 66 cases of Hodgkin lymphoma diagnosed at Liaquat National Hospital over a duration of 2 years from January 2014 to December 2015. The slides and blocks of all cases were retrieved, and representative blocks were selected for LMP1 by IHC. LMP1 expression of >10% of cells was considered as positive expression and correlated with histologic subtypes and clinical parameters like age, gender, and site of involvement.ResultsThe mean age of patients was 35.11 (+20.22). LMP1 expression was found in 68.1% (45/66) of cases of Hodgkin lymphoma. Mean age of the patients with LMP1 expression was 32.04 (+21.02). LMP1 expression was found in 40% cases of lymphocyte-rich, 66.7% of lymphocyte-depleted, 73.9% of mixed cellularity, 66.7% of nodular sclerosis, and 73.7% of classic Hodgkin lymphoma, NOS. No significant correlation of LMP1 expression with any clinical or histological parameter could be established in our studied patient population.ConclusionsA high frequency of expression of LMP1 is seen in cases of Hodgkin lymphoma at our setup comparable to endemic regions of the world; therefore, preventive and treatment protocols should be designed accordingly.

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“… Methodology (PCR versus in situ hybridization): we used a sensitive method for detection of EBV DNA with positive and negative controls. Based on the previous studies, PCR is as sensitive as in situ hybridization [ 20 , 21 ]. However, as we did not use in situ hybridization, we could not localize the exact infected cell with EBV in our controls which might be the circulating B cells of the skin instead of keratinocytes.…”

Section: Discussionmentioning

confidence: 99%

Macular Amyloidosis and Epstein-Barr Virus

Nahidi

Meibodi

Meshkat

et al. 2016

Dermatology Research and Practice

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Background. Amyloidosis is extracellular precipitation of eosinophilic hyaline material of self-origin with special staining features and fibrillar ultrastructure. Macular amyloidosis is limited to the skin, and several factors have been proposed for its pathogenesis. Detection of Epstein-Barr virus (EBV) DNA in this lesion suggests that this virus can play a role in pathogenesis of this disease. Objective. EBV DNA detection was done on 30 skin samples with a diagnosis of macular amyloidosis and 31 healthy skin samples in the margin of removed melanocytic nevi by using PCR. Results. In patients positive for beta-globin gene in PCR, BLLF1 gene of EBV virus was positive in 23 patients (8 patients in case and 15 patients in the control group). There was no significant difference in presence of EBV DNA between macular amyloidosis (3.8%) and control (23.8%) groups (P = 0.08). Conclusion. The findings of this study showed that EBV is not involved in pathogenesis of macular amyloidosis.

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Comparison of three methods for the detection of Epstein-Barr virus in Hodgkin’s lymphoma in paraffin-embedded tissues

Cited by 30 publications

References 16 publications

Establishing etiopathogenesis in Epstein-Barr virus associated malignancies using chromogenic in situ hybridization

Establishing etiopathogenesis in Epstein-Barr virus associated malignancies using chromogenic in situ hybridization

Latent membrane protein 1 (LMP1) expression in Hodgkin lymphoma and its correlation with clinical and histologic parameters

Macular Amyloidosis and Epstein-Barr Virus

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