Comparison of three immunoassays and one GC-MS method for the determination of valproic acid

Cooreman, S.; Cuypers, Eric; Doncker, Mireille De; hee, Paul Van; Uyttenbroeck, Wim; Neels, Hugo

doi:10.1016/j.immbio.2008.01.004

Cited by 8 publications

(3 citation statements)

References 7 publications

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“…In the relevant consensus guidelines, the effective therapeutic concentration of VPA ranges from 50 to 100 μg/ml and the laboratory alert value is 120 μg/ml (Hiemke et al, 2018). The individual medication of VPA during treatment can improve the control rate of epilepsy and reduce adverse reactions (Gerstner, Bell, & Konig, 2008;Yukawa, 1996).To date, several methods for the quantification of VPA concentrations in biological fluids have been developed, such as immunoassay (Cooreman et al, 2008), liquid chromatography-mass spectrometry (LC-MS) (Gao et al, 2011;Zhao et al, 2016), high-performance liquid chromatography (HPLC) (Mao, Zhao, Sun, Lu, & Li, 2019) and gas chromatography (GC) (Shahdousti, Mohammadi, & Alizadeh, 2007). Among these methods, immunoassay is the most commonly used method in clinical laboratories, while lacking selectivity.…”

Section: Introductionmentioning

confidence: 99%

A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

Liu

Shang

Yao

et al. 2019

Biomedical Chromatography

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A novel and robust two‐dimensional liquid chromatography with ultraviolet detection method (2D‐LC–UV) was developed and validated for high‐throughput determination of the concentrations of valproic acid (VPA) in human plasma. This 2D‐LC system was composed of a first‐dimensional LC column, a second‐dimensional LC column and an intermediate transfer column. The sample was directly injected into the 2D‐LC system after an easy protein precipitation treatment. After online preconcentration and primary separation by the first‐dimensional column, the target was captured by an intermediate column and then transferred to second‐dimensional column for analysis. The system transferred the target through “central cutting” mode whereby the drug peak was not subject to interference from the matrix. The analysis cycle time was completed within 7.0 min. Compared with other methods that have been developed, the analysis time was reduced and the operation was much easier without any derivatization. The calibration curve was linear over the 5.90–188.94 μg/ml range for the VPA concentrations. The intra‐day and inter‐day precisions were <5.6%. The recoveries were in the range from 95.2 to 98.0%. This method appears to be sensitive, precise, rapid and low‐cost for the quantification of VPA in serum sample.

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Section: Introductionmentioning

confidence: 99%

A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

Liu

Shang

Yao

et al. 2019

Biomedical Chromatography

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“…Additionally, due to VPA volatility, GC is often used. 20,21 In order to prevent severe tailing of the fatty acid peak, on-column and pre-column derivatization has also been used. 22 Because VPA is a relatively strong organic acid, it does not chromatograph well on the standard nonpolar or slightly polar stationary phases routinely applied to clinical GLC determination of other anticonvulsant drugs unless first derivatized.…”

Section: Introductionmentioning

confidence: 99%

A rapid and simple procedure for monitoring valproic acid by gas chromatography

2018

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Valproic acid (VPA), a widely used antiepileptic drug, has a narrow therapeutic range of 50-100 μg/mL and shows large individual variability. It is very important to monitor the trough VPA concentration using a reliable method. The aim of this study was to develop and validate a rapid gas chromatographic (GC) technique for VPA quantification in human plasma and to compare it with the traditional immunoassay method. VPA extraction from human serum was efficient by dichloromethane and hydrochloric acid using octanoic acid as an internal standard. GC analysis was performed using a gas-chromatograph equipped with a flame ionization detector (GC/FID). VPA detection and quantification were accomplished isothermally at 135°C on a Gs-BP 100% dimethylpolysiloxane capillary column (10 m×0.53 mm ID, 2.65 μm film thickness, Supelco, Bellefonte, PA). Injection port and detector temperature were 280°C. Retention times of VPA and internal standard were 1.83 min and 2.33 min, respectively. The calibration curve was linear over the concentration range of 5-320 μg/mL, with a lower limit of detection of 1.25 μg/mL. The internal and inter-day precision was less than 5.3% and 6.1%, respectively, and the accuracy was below 2.8%. VPA recovery was 94.6%. A quick and accurate method for VPA determination in human plasma was developed and validated. It resulted sufficiently selective and sensitive.

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“…In both approaches for the quantification step the most commonly used methods are enzyme immunoassays [17,18]. This technique is simple and reliable, but relatively expensive.…”

Section: Introductionmentioning

confidence: 99%

Determination of free and total valproic acid in human plasma by capillary electrophoresis with contactless conductivity detection

Pham

See

Morand

et al. 2012

Journal of Chromatography B

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Comparison of three immunoassays and one GC-MS method for the determination of valproic acid

Cited by 8 publications

References 7 publications

A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

A rapid and simple procedure for monitoring valproic acid by gas chromatography

Determination of free and total valproic acid in human plasma by capillary electrophoresis with contactless conductivity detection

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