Comparison of three different techniques to diagnose Fasciola hepatica infection in experimentally and naturally infected sheep

A review of bovine fasciolosis and other trematode infections in Nigeria

“…hepatica infection (Martínez-Pérez et al, 2012). Molecular identification of paramphistome species from Asia and elsewhere in Africa has been carried out (Lotfy et al, 2010), however at time of writing this review, there are no records of such studies having been carried out in Nigeria.…”

Molecular diagnosis of natural fasciolosis by DNA detection in sheep faeces

“…But sometimes, animals suffering from fasciolosis do not show specific clinical signs, or symptoms are present when the infection is due to another disease, or the course of fasciolosis is subclinical (Rojo-Vázquez et al 2012).…”

“…Recently, PCR and nested-PCR methods have been developed to detect for the presence of F. hepatica in faeces of sheep (Martínez-Pérez et al 2012;Roble-Pérez et al 2013), based on the amplification of the coding region of cox1 gene, regions of the cox1 gene and the large ribosomal RNA subunit, or the ribosomal internal transcribed spacer 2 in the mitocondrial DNA. Martínez-Pérez et al (2012) compared different techniques for the early diagnosis of the infection by F. hepatica in experimentally and naturally infected sheep, including coprological method a commercial immunoassay, standard PCR and nested PCR.…”

“…PCR assays of faeces are highly sensitive for the diagnosis of the infection due to platyhelminth parasites; i.e. Echinococcus spp., Taenia spp., F. hepatica, F. gigantica, using PCR assays of faeces (Bretagne et al 1993;Dinkel et al 1998;Naidich et al 2006;Mathis and Deplazes 2006;Ai et al 2010;Martínez-Pérez et al 2012;Robles-Pérez et al 2013). Previously developed PCR and nested-PCR methods to detect the presence of F. hepatica in faeces of sheep (Martínez-Pérez et al 2012;Roble-Pérez et al 2013) have employed fresh samples and commercial kits for DNA extraction.…”

FAMACHA © : A potential tool for targeted selective treatment of chronic fasciolosis in sheep

“…Unfortunately, few pragmatic markers exist for the level of liver fluke infection in individual hosts. Faecal trematode egg counts, detection of parasite antigen or DNA in faeces, levels of liver enzymes or anti-parasite antibodies in blood, and levels of anti-parasite antibodies in milk, can all help to estimate levels of infection at herd or flock level, but are inaccurate, expensive and impractical at the level of the individual (Gordon et al, 2012b;Martínez-Pérez et al, 2012;Rojas et al, 2014).…”