Comparison of three different methods for the quantification of equine insulin

Warnken, Tobias; Huber, Korinna; Feige, Karsten

doi:10.1186/s12917-016-0828-z

Cited by 60 publications

(56 citation statements)

References 24 publications

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“…It has been well described that, as most insulin assays are immunoassays, the amount of immunoreactive insulin detected by each assay can vary and that a simple correction factor cannot be used to easily compare concentrations obtained with 1 assay with concentrations obtained with another assay. 17,24 Therefore, this limitation was addressed by using the percent change in insulin concentration rather than the actual measured insulin concentration. An additional limitation was the use of a 24-hour washout period between alpha-2-agonist testing periods.…”

Section: Discussionmentioning

confidence: 99%

“…Plasma was collected immediately, whereas blood was allowed to clot for 45 minutes at room temperature, centrifuged, and serum was extracted. Depending on the institution, insulin was measured using a radioimmunoassay (Institution 1) or a chemiluminescent assay (Institution 2) previously validated in horses 16,17 . Blood glucose was measured using a glucohexokinase colorimetric assay as previously described 14 .…”

Section: Methodsmentioning

confidence: 99%

“…Depending on the institution, insulin was measured using a radioimmunoassay (Institution 1) or a chemiluminescent assay (Institution 2) previously validated in horses. 16,17 Blood glucose was measured using a glucohexokinase colorimetric assay as previously described. 14 A 24-hour washout period was observed before the same procedure was followed with the other alpha-2-agonist.…”

Section: Horsesmentioning

confidence: 99%

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Blood glucose and insulin concentrations after alpha‐2‐agonists administration in horses with and without insulin dysregulation

Kritchevsky

Muir

Leschke

et al. 2020

Veterinary Internal Medicne

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Background In metabolically stable horses, alpha‐2‐agonists suppress insulin secretion with transient hyperglycemia and rebound hyperinsulinemia. In horses with insulin dysregulation (ID), the effect of alpha‐2‐agonists has not been investigated; however, both the alpha‐2‐agonist‐induced suppression of insulin secretion and rebound hyperinsulinemia could have clinical relevance. Hypothesis/Objectives In horses with ID, alpha‐2‐agonists will alter insulin and glucose dynamics. Animals Seven horses with ID and 7 control horses. Methods In this randomized crossover study, xylazine hydrochloride (1.1 mg/kg) or detomidine hydrochloride (30 μg/kg) were administered IV, and blood was collected for glucose and insulin concentrations at 0, 15, 30, 45, 60, 90, 120, 150, 180, and 300 minutes after administration. Horses received each drug in a random order with a 24‐hour washout period between drugs. Percent change in glucose and insulin concentrations was compared between groups, drugs, and over time with P < .05 considered significant. Results A significant time‐dependent effect of both alpha‐2‐agonists on glucose and insulin concentrations in control and ID horses was identified (P = .01 for all comparisons). There was no significant effect of sedative selection and endocrine status on blood glucose concentration in either group; however, in ID horses, xylazine administration resulted in severe rebound hyperinsulinemia whereas detomidine administration did not (P = .02). Conclusions and Clinical Importance Alpha‐2‐agonists have a significant effect on glucose and insulin concentrations in horses. In ID horses, detomidine could minimize hyperinsulinemia when compared to xylazine.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Horsesmentioning

confidence: 99%

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Blood glucose and insulin concentrations after alpha‐2‐agonists administration in horses with and without insulin dysregulation

Kritchevsky

Muir

Leschke

et al. 2020

Veterinary Internal Medicne

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“…Farm animal and pet welfare is the main goal of veterinary medicine for both economic and affection reasons. Nowadays, the performance of animal diagnostic serology is often suboptimal, mostly due to the low availability of commercial species‐specific immunoassays . Even though clinical care may capitalize from what has already been discovered by human system biology, dedicated biomarker discovery and validation efforts would be needed for improving veterinary diagnostics .…”

Section: Numbers Of Identified Proteins Peptides Psms and Search Imentioning

confidence: 99%

“…species-specific immunoassays. [1] Even though clinical care may capitalize from what has already been discovered by human system biology, dedicated biomarker discovery and validation efforts would be needed for improving veterinary diagnostics. [2] Nevertheless, compared to humans, biomarker discovery studies directly performed on animal specimens lag considerably behind.…”

Section: Doi: 101002/pmic201800191mentioning

confidence: 99%

All Cats are Gray in the Dark: Enrichment/Depletion Approaches for Biomarker Discovery on Felis catus Plasma

et al. 2018

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In veterinary medicine, assay performance is often affected by the lack of species-specific diagnostic tools. Reliable biomarkers might be identified by investigating biological fluids of the species of interest, but protein sequence databases are often incomplete and human-specific devices for reducing sample complexity might fail when applied to animal plasma. Here, seven commercial methods based on different capturing agents (anti-human antibodies, affinity ligands, mixture of antibodies and ligands, and combinatorial peptide ligand libraries) are applied to cat plasma and evaluated in terms of yield, identified proteins/ peptides, and relative abundance by high-resolution shotgun proteomics and label-free quantitation. As a result, anti-human antibody-based methods are unsatisfactory. Most fail in reducing albumin and immunoglobulins, and some lead to a substantial removal of other highly abundant proteins, probably because of nonspecific interactions. A protein A/dye ligand-based method is efficient in reducing immunoglobulins, fibrinogen, and apolipoprotein A1 and A2, but not albumin, and protein identifications do not increase. Only peptide ligand libraries flatten the dynamic range, and increased protein identification (59.0%). Albumin and immunoglobulins are successfully depleted (60.7% and 35.9%, respectively). Although further studies will be required for reinforcing our observations, this work can provide a useful guide for cat plasma pretreatment in biomarker discovery studies.

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Laboratory Assessment of Lipid and Glucose Metabolism

Walton¹

2020

Equine Hematology, Cytology, and Clinical Chemistry

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Comparison of three different methods for the quantification of equine insulin

Cited by 60 publications

References 24 publications

Blood glucose and insulin concentrations after alpha‐2‐agonists administration in horses with and without insulin dysregulation

Blood glucose and insulin concentrations after alpha‐2‐agonists administration in horses with and without insulin dysregulation

All Cats are Gray in the Dark: Enrichment/Depletion Approaches for Biomarker Discovery on Felis catus Plasma

Laboratory Assessment of Lipid and Glucose Metabolism

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