Comparison of ThinPrep and SurePath liquid‐based cytology and subsequent human papillomavirus DNA testing in China

Zhao, Fanghui; Hu, Shangying; Bian, Jessica J; Liu, Bin; Peck, Roger; Bao, Yanping; Pan, Qin-Jing; Frappart, Lucien; Sellors, John; Qiao, You‐Lin

doi:10.1002/cncy.20177

Cited by 45 publications

(30 citation statements)

References 27 publications

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“…Both are considered valid technologies, but SurePath has a slightly higher positive predictive value (PPV). 23,24 Given the low prevalence of cervical abnormality in the study population, the results were adjusted for laboratory. There are other known risk factors for HPV infection and cervical cancer, such as smoking.…”

Section: Covariatesmentioning

confidence: 99%

Effect of human papillomavirus vaccination on cervical cancer screening in Alberta

Kim

Bell

Sun

et al. 2016

CMAJ

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Background: A school-based program with quadrivalent human papillomavirus (HPV) vaccination was implemented in Alberta in 2008. We assessed the impact of this program on Pap test cytology results using databases of provincewide vaccination and cervical cancer screening. Methods:We conducted a nested case-control study involving a cohort of women in Alberta born between 1994 and 1997 who had at least 1 Pap test between 2012 and 2015. Women with negative cytology results were controls. Women with low-grade (atypical squamous cells of undetermined significance or low-grade squamous intraepithelial lesion) and highgrade (atypical squamous cells, cannot rule out a high-grade lesion; or high-grade squamous intraepithelial lesion) cervical abnormalities were cases. Exposure status was assigned according to records of HPV vaccination. Odds ratios (ORs) for abnormal cytology results by vaccination status were adjusted for neighbourhood income, laboratory service, rural versus urban residency, and age. Results:The total study population was 10 204. Adjusting for age, vaccinated women had a higher screening rate than unvaccinated women (13.0% v. 11.4%, p < 0.001). Among women who received full vaccination (≥ 3 doses), the adjusted OR for cervical abnormalities was 0.72 (95% confidence interval [CI] 0.63-0.82). For high-grade lesions, the adjusted OR was 0.50 (95% CI 0.30-0.85). With 2-dose HPV vaccination, the adjusted OR for cervical abnormalities was 1.08 (95% CI 0.84-1.38).Interpretation: Quadrivalent HPV vaccination significantly reduced high-grade cervical abnormalities but required 3 doses. Vaccination against HPV was associated with screening uptake. Population-based vaccination and screening programs should work together to optimize cervical cancer prevention. AbstractResearch

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Section: Covariatesmentioning

confidence: 99%

Effect of human papillomavirus vaccination on cervical cancer screening in Alberta

Kim

Bell

Sun

et al. 2016

CMAJ

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“…In addition, we estimated that <5% of samples after completion of the conventional Pap smear and for which we must conduct a search for HPV infection contained <2 ml of residual liquid. In the literature, we find a rate of up to 18.2% [8]. This can be a problem when setting up a systematic screening of HPV associated with conventional cytology (small volumes remaining to research HPV).…”

Section: Discussionmentioning

confidence: 99%

Importance of the Residual Volume of the Cytological Solution for the Reproducibility of the Hybrid Capture 2 High-Risk Human Papillomavirus DNA Test

Catteau

Vanhaeverbeek²,

Noël³

2012

Acta Cytologica

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Objective: Human papillomavirus (HPV) infections are widespread in all human populations, and a large number of studies have demonstrated a relationship between high-risk (HR)-HPV infections and cervical intraepithelial neoplasia or invasive cervical cancer. To the best of our knowledge, no independent study clearly demonstrates the importance of the quantity of residual liquid after cytology in terms of sensitivity for HR-HPV detection. Study Design: We conducted a study to assess the relationship between the liquid-based cytology volume and the sensitivity of the hybrid capture 2 test for the detection of HR-HPV in 23 cytological high-grade squamous intraepithelial lesions with a cervical intraepithelial neoplasia grade 2 or worse protocol on biopsy. Results: Although the sensitivity of the tests showed no statistically significant differences, we still found a significant variation in the median values of relative light units/control according to the amount of liquid used. Conclusions: If 2 ml of liquid is used, this could lead to false negatives when the value of relative light units/control is only slightly greater than 1. The fact that we found a lower viral load at 2 versus 4 or 8 ml was also important in terms of predicting the evolution of cervical lesions. We recommend using at least 4 ml of the PreservCyt solution for HR-HPV detection with the hybrid capture 2 test.

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“…With the emerging evidence suggesting that LBC is not superior in disease detection over conventional cytology methods in recently published data, the performance of LBC will undoubtedly be challenged when considering escalating costs in the laboratory [6,10,13,17]. Further, it has been suggested that LBC is equivalent to HPV testing in disease detection and others have advocated for adoption of LBC in terms of a more efficient use of automated screening modalities [18].…”

Section: Discussionmentioning

confidence: 99%

“…Some have shown the relative benefits of LBC, including increased sensitivity, especially for the detection of cervical high-grade squamous intraepithelial lesions (HSIL), and the ability to perform HPV testing on the same specimen. Other studies have reported decreased specificity and lower cost-effectiveness [6,10,13]. Nonetheless, the benefits of LBC over conventional cytology include better specimen preservation and presentation, and a higher number of cells on the slide.…”

Section: Introductionmentioning

confidence: 99%

Performance of the CellSolutions Glucyte Liquid-Based Cytology in Comparison with the ThinPrep and SurePath Methods

Alaghehbandan

2013

Acta Cytologica

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Objective: Glucyte liquid-based cytology (LBC; BestPrep™;CellSolutions, LLC, Greensboro, N.C., USA) is a recently developed method. Its performance was assessed in comparison with ThinPrep (Hologic Inc., Marlborough, Mass., USA) and SurePath (BD Diagnostics, Burlington, N.C., USA) LBC platforms. Study Design: Two cervical samples obtained from each of 331 patients presenting for colposcopy were utilized. The first sample was placed in PreservCyt (Hologic) and the second in SurePath medium, and both were processed in accordance with the manufacturers’ protocols. From the residual SurePath sample, a Glucyte slide was prepared, stained, and read as per the manufacturer’s instructions. Results: Considering a threshold cytologic diagnosis of atypical squamous cells of undetermined significance and using a histologic diagnosis of high-grade cervical intraepithelial neoplasia or worse (CIN 2+) as the end point, there was no significant difference in the sensitivity of Glucyte for the detection of CIN 2+ compared with ThinPrep and SurePath (86.9 vs. 81.9% and 83.7%, respectively). However, Glucyte showed a specificity of 49.2% compared with 61.6% for ThinPrep (p = 0.002) and 66.9% for SurePath (p < 0.001). Considering a threshold cytologic diagnosis of high-grade squamous intraepithelial lesion, Glucyte showed a sensitivity of 40.5% for detecting CIN 2+ compared with 20.5% for ThinPrep (p = 0.003) and 54.7% for SurePath (p = 0.013). The corresponding specificities were 93.8, 99.1, and 94.3%. The rate of unsatisfactory specimens for Glucyte was 1.2% compared with ThinPrep (4.8%) and SurePath (0%). Conclusion: Glucyte appeared to perform reasonably well compared with ThinPrep and SurePath. It offers an alternative to these well recognized LBC systems.

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Comparison of ThinPrep and SurePath liquid‐based cytology and subsequent human papillomavirus DNA testing in China

Abstract: BACKGROUND:Liquid-based cytology (LBC) has been compared with conventional cytology in numer-

Cited by 45 publications

References 27 publications

Effect of human papillomavirus vaccination on cervical cancer screening in Alberta

Effect of human papillomavirus vaccination on cervical cancer screening in Alberta

Importance of the Residual Volume of the Cytological Solution for the Reproducibility of the Hybrid Capture 2 High-Risk Human Papillomavirus DNA Test

Performance of the CellSolutions Glucyte Liquid-Based Cytology in Comparison with the ThinPrep and SurePath Methods

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