Comparison of the Wound Healing Effect of Cellulose and Gelatin: An In Vivo Study

Abstract

BackgroundMany topical hemostatics are widely applied for bleeding control. They can be classified into two categories according to their mechanism of action on the clotting cascade in a biologically active or passive manner. Passive hemostatics include cellulose and gelatin. We performed an experimental study to compare the effect of passive hemostatics in wound healing by applying them to a rectus abdominis muscle defect of white mice.MethodsSurgicel is a sterile absorbable knitted fabric prepared by the controlled oxidation of regenerated cellulose. Spongostan is an absorbable hemostatic gelatin sponge. In 30 mice, a 1×1 cm defect was created on the rectus abdominis muscle and the materials were applied in three ways: control group, cellulose (Surgicel) group, gelatin (Spongostan) group. For the histologic analysis, biopsies were performed at 3 and 28 days.ResultsAfter 3 days, the cellulose group showed limited granulation formation with acute inflammatory reactions similar to the control group. At the 28th day, moderate amounts of granulation tissue formation was observed with milder inflammatory reactions than the control group. In the gelatin group, after 3 days, gelatin remnants were observed surrounded by severe inflammatory changes. After 28 days, the same quantity of gelatin remnants could be still observed.ConclusionsThis study suggests that cellulose is associated with minimal morbidity in wound healing, while the use of gelatin shows severe adverse tissue reactions with delayed wound healing. Consequently, cellulose is better than gelatin when considering wound healing.

Effect of oxidized cellulose on human respiratory mucosa and submucosa and its implications for endoscopic skull‐base approaches

“…The low pH induced in the culture medium appears to be the cause for this effect, because neutralizing the lowered pH reverses the effect of ROC on cell viability. The same mechanism was reported by Kang et al for ROC‐induced nerve damage . Because cell viability is essential in the healing process, these data argue against the healing‐inductive properties of ROC, especially in the setting of a vascularized flap during EESBS.…”

“…The same mechanism was reported by Kang et al for ROCinduced nerve damage. 35 Because cell viability is essential in the healing process, these data argue against the healinginductive properties of ROC, especially in the setting of a vascularized flap during EESBS.…”

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

“…Cellulose derivatives, such as CMC and MC, ethyl cellulose, acetyl cellulose, and hydroxypropyl cellulose have frequently been used to formulate hydrogels, 110 nanoparticles (e.g., nanowhiskers), 111 and nanofibers. 112 Moreover, in combination with other synthetic and natural polymers, such as proteins, 113 in particular gelatin, 114-119 a wide range of applications [120][121][122][123][124][125][126] have been demonstrated for these composite materials as scaffolds for 3D cellular engineering. 127,128 Glycosaminoglycans (GAGs) in the ECM may be mimicked by electrospinning partially sulfated cellulose with gelatin, yielding functional fibrous structures.…”

Assessment of Suturing Technique, Gelatin Sponge and Aluminum Chloride in Controlling Hepatic Bleeding and Abdominal Adhesions in Dogs

“…The gelatin remnants can induce a foreign body reaction, chronic inflammation, or infection leading to formation of granuloma and interfere with the wound healing process (Samudrala, 2008). (Kang et al, 2012) found that gelatin delays wound healing. Herein; we noticed a marked fibrin deposition within the clot accompanied over granulation tissue formation.…”