Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis

Mohammadzadeh, Tahereh; Sako, Yasuhito; Sadjjadi, Seyed Mahmoud; Sarkari, Bahador; Itō, Akira

doi:10.1016/j.trstmh.2012.01.012

Cited by 43 publications

(46 citation statements)

References 21 publications

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“…More recently, synthetic peptides or recombinant antigens from the sequences of two major components of HCF (antigen B and antigen 5) have been obtained (9,(11)(12)(13). These new antigens have a better performance than their predecessors and are more reproducible across populations, improving test reliability and allowing a better test standardization (6,9,11,13). Recombinant antigen B8/ 1(rAgB) seems to have a good diagnostic performance in an ELISA format with sensitivity between 94.6% and 95.8, and specificity between 93.9% and 100% (13,14).…”

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confidence: 99%

Evaluation of a New Immunochromatographic Test Using Recombinant Antigen B8/1 for Diagnosis of Cystic Echinococcosis

et al. 2015

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i Diagnosis of cystic echinococcosis (CE) is based on the identification of the cyst(s) by imaging, using immunodiagnostic tests mainly as complementary tools in clinical settings. Among the antigens used for immunodiagnosis, previous studies described a good performance of the recombinant antigen B8/1 (rAgB) in an enzyme-linked immunosorbent assay (ELISA) format; however, in remote parts of areas where the disease is endemic, the implementation of an ELISA is difficult, so a more simple, rapid, and reliable method such as the immunochromatographic test (ICT) is required. In this study, using a set of 50 serum samples from patients with surgically confirmed CE, we compared the performance of an ICT and that of an ELISA using the rAgB. The overall sensitivities of ICT and ELISA were not statistically different (78% versus 72%; P ‫؍‬ 0.36). The overall agreement between both tests was moderate ( ‫؍‬ 0.41; P < 0.01). Concordance between ICT and ELISA was substantial or almost perfect for patients with liver involvement ( ‫؍‬ 0.65; P < 0.001) and patients with more than one hydatid cyst ( ‫؍‬ 0.82; P < 0.001), respectively. Moreover, specificity analysis using a total of 88 serum samples from healthy individuals (n ‫؍‬ 20) and patients (n ‫؍‬ 68) with other parasitic infections revealed that ICT had a specificity of 89.8%. ICT and ELISA had similar performance for the detection of specific antibodies to E. granulosus, and ICT had a high specificity, opening the possibility of using ICT as a screening tool in rural settings. Cystic echinococcosis (CE) is a zoonotic disease caused by the larval stage of the dog tapeworm Echinococcus granulosus. This zoonosis has a worldwide distribution, being considered a public health problem in areas dedicated to the raising of livestock where CE is endemic (1). In these areas, risk factors such as access of dogs to contaminated viscera and close contact between infected dogs and humans facilitate and maintain the transmission of the disease (2, 3). Humans are infected by the accidental ingestion of the tapeworm eggs, which can develop to the larval stage (hydatid cyst) in any internal organ after several years. The organs more frequently involved are the liver and the lungs, representing ϳ70% and ϳ20% of cases, respectively (4). Diagnosis of CE is based on the identification of the hydatid cyst(s) by imaging methods (e.g., abdominal ultrasound, chest X ray, or computed tomography) (5). Immunodiagnostic tools are of use in clinical settings as a complementary diagnostic tool and have quite variable performance, which depends on the antigen or technique used and is affected by certain disease characteristics, such as cyst location and presence of cyst rupture or aggregated bacterial infection (6-9).Among the antigens used for immunodiagnostic, hydatid cyst fluid (HCF) has been the one most widely used. Antibody-detecting assays using HCF report sensitivities between 75% and 95% with poor specificity and frequent cross-reactions (5, 9-11). More recently, synthetic peptides or recombi...

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Evaluation of a New Immunochromatographic Test Using Recombinant Antigen B8/1 for Diagnosis of Cystic Echinococcosis

et al. 2015

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“…Several serological tests based on HCF and its immunogenic сomponents have been developed recently for diagnosing human CE (Tawfeek et al 2011, Mohammadzadeh et al 2012, Jin et al 2013, Pagnozzi et al 2014. Unfortunately, the immunological tests available today are not satisfactory and approaches to the serological diagnosis of CE are still the subject of discussion (Sarkari and Rezaei 2015).…”

Section: Discussionmentioning

confidence: 99%

“…HCF is a rich reservoir of excretory-secretory antigens (ES-Ag) of protoscoleces containing a wide range of proteins from both parasite and host origin (Aziz et al 2011). Over the last five years a number of immunodiagnostic tests based on HCF and its individual components such as AgB as well as Ag5 were developed for serodiagnosis of human CE (Tawfeek et al 2011, Mohammadzadeh et al 2012, Pagnozzi et al 2014. However, their efficacy in the diagnosis of human hydatid disease remains low (Sarkari and Rezaei 2015).…”

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Serological diagnosis of cystic echinococcosis in cattle

Bulashev¹,

Suranshiev²,

Akibekov³

et al. 2017

FOLIA PARASIT

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An IgM murine monoclonal antibody (MAb) was obtained against the excretory-secretory antigen (ES-Ag) of in vitro reared protoscoleces of Echinococcus granulosus (Batsch, 1786). Western blotting revealed that the MAb recognised a 20.6 kDa protein of this ES-Ag. The MAb was used in sandwich enzyme-linked immunosorbent assay (s-ELISA) for selective sensitisation of the solid phase with the protoscolex-specific protein from its ES-Ag and somatic antigen (S-Ag) to examine serum samples of 108 cows from a cystic echinococcosis (CE) endemic area for specific antibodies and to compare the results with those from necropsies and an indirect ELISA (i-ELISA). The sensitivity of s-ELISA/ES-Ag, s-ELISA/S-Ag and i-ELISA/S-Ag was 48%, 52% and 62%, respectively. The low sensitivity of the ELISA was probably caused by the fact that 13 cows (62%) were infected with sterile cysts (acephalocysts and/or calcified foci) only. A relatively high specificity (80%) of s-ELISA/ES-Ag was observed in cows with fertile cysts. It also detected antibodies in the serum of two cows that had recovered from the disease according to the necropsy. The i-ELISA/S-Ag gave false results in testing sera from a healthy animal and from a cow with tubercular foci. Further analysis will be necessary to define more precisely the value of this study, because the duration of antibody elimination from the bloodstream of recovered cattle remains unknown. The solution of this problem will increase the specificity of the proposed test in monitoring herbivorous animals for CE.

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“…The sample was boiled for 15 minutes and centrifuged at 50,000 + g for 1 hour to separate heat-stable AgB in the supernatant from other components in the pellet. 11,22 AgB concentration was determined using a commercially available protein assay (Bio-Rad, Hercules, CA) based on the method by Bradford. 23 Purified AgB was stored at −80 C until required.…”

Section: Methodsmentioning

confidence: 99%

“…9 However, assay sensitivity and specificity are variable owing to the different antigens and methods used. 6,10,11 Human hydatid cyst fluid is deemed unsuitable for diagnostic purposes because of the presence of human proteins, such as immunoglobulin G (IgG). In addition to the parasite protein, some human IgG may also bind to the solid support in an immunoassay and potentially react with the anti-human IgG secondary antibody.…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Test Using Echinococcus granulosus Native Antigen B and Comparison of IgG and IgG4 Dipsticks for Detection of Human Cystic Echinococcosis

Khalilpour¹,

Sadjjadi²,

Moghadam³

et al. 2014

The American Journal of Tropical Medicine and Hygiene

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Abstract. Cystic echinococcosis (CE) caused by infection with Echinococcus granulosus is of major concern for humans in many parts of the world. Antigen B was prepared from E. granulosus hydatid fluid, and Western blots confirmed eight batches showing a band corresponding to the 8-/12-kDa subunit with positive serum and no lowmolecular mass band ( 15 kDa) with negative serum. The batches were pooled and used to prepare lateral flow immunoglobulin G4 (IgG4) and IgG dipsticks. Diagnostic sensitivity was determined using serum samples from 21 hydatidosis patients, and diagnostic specificity was established using sera from 17 individuals infected with other parasites and 15 healthy people. IgG4 dipstick had a diagnostic sensitivity of 95% (20 of 21) and a specificity of 100% (32 of 32). The IgG dipstick had a sensitivity of 100% (21 of 21) and a specificity of 87.5% (28 of 32). Thus, both IgG and IgG4 dipsticks had high sensitivities, but IgG4 had greater specificity for the diagnosis of human CE.

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Comparison of the usefulness of hydatid cyst fluid, native antigen B and recombinant antigen B8/1 for serological diagnosis of cystic echinococcosis

Cited by 43 publications

References 21 publications

Evaluation of a New Immunochromatographic Test Using Recombinant Antigen B8/1 for Diagnosis of Cystic Echinococcosis

Evaluation of a New Immunochromatographic Test Using Recombinant Antigen B8/1 for Diagnosis of Cystic Echinococcosis

Serological diagnosis of cystic echinococcosis in cattle

Lateral Flow Test Using Echinococcus granulosus Native Antigen B and Comparison of IgG and IgG4 Dipsticks for Detection of Human Cystic Echinococcosis

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