Comparison of the two techniques for measurement of the density of <i>Demodex folliculorum</i>
: standardized skin surface biopsy and direct microscopic examination

Aşkin, Ülkü; Seckın, Denız

doi:10.1111/j.1365-2133.2010.09645.x

Cited by 73 publications

(54 citation statements)

References 12 publications

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“…The diagnosis of demodicosis was made when compatible clinical manifestations of demodicosis was combined with a high Dd (>5D/cm 2 ) by SSSB620 or DME192324.…”

Section: Methodsmentioning

confidence: 99%

“…Two methods are commonly used to determine Demodex mite densities–a standardized skin surface biopsy (SSSB) and direct microscopic examination (DME). A recent study found SSSB is more effective than DME for detecting Demodex 19, but no study has previously compared the two methods with respect to the clinical types and distribution patterns of demodicosis. Accordingly, the aim of this study was to compare measures of Demodex mite densities obtained using SSSB and DME in terms of their relations with clinical types and distribution patterns.…”

Section: Introductionmentioning

confidence: 99%

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DemodexMite Density Determinations by Standardized Skin Surface Biopsy and Direct Microscopic Examination and Their Relations with Clinical Types and Distribution Patterns

Yun

Yun²,

Baek

et al. 2017

Ann Dermatol

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BackgroundDemodicosis is a parasitic skin disease caused by Demodex mites, and the determination of mite density per square centimeter is important to diagnose demodicosis. Standardized skin surface biopsy (SSSB) and direct microscopic examination (DME) are commonly used to determine Demodex mites density (Dd). However, no study has previously compared these two methods with respect to clinical types and distribution patterns of demodicosis.ObjectiveThe aim of this study was to compare the value of SSSB and DME findings in reference to the clinical types and distribution patterns of demodicosis.MethodsThe medical records of 35 patients diagnosed with demodicosis between December 2011 and June 2015 were retrospectively reviewed. Demodicosis was classified according to four clinical types (pityriasis folliculorum, rosacea type, acne type, and perioral type) and three distribution patterns (diffuse pattern, U-zone pattern, and T-zone pattern). Two samples, one for SSSB and one for DME, were obtained from a lesion of each patient.ResultsIn all patients, mean Dd and the proportion with a high Dd (>5D/cm2) by DME (14.5±3.3, 80.0%, respectively) were higher than by SSSB (5.5±1.3, 37.1%, respectively; p<0.01, p=0.02, respectively). In terms of clinical types, for rosacea type, mean Dd and proportion with a high Dd by DME (12.4±3.5, 84.6%, respectively) were significantly greater than those determined by SSSB (3.6±1.2, 23.1%; p=0.04, p=0.04, respectively). In terms of distribution pattern, for the diffuse pattern, mean Dd and the proportion with a high Dd by DME (17.5±3.7, 100%, respectively) were significantly higher than those determined by SSSB (6.0±2.7, 26.7%; p<0.01, p<0.01, respectively).ConclusionThe results of our study revealed that DME is a more sensitive method for detecting Demodex than SSSB, especially in patients with diffuse pattern and suspected rosacea type. Further research is needed to confirm this finding.

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“…The diagnosis of demodicosis was made when compatible clinical manifestations of demodicosis was combined with a high Dd (>5D/cm 2 ) by SSSB620 or DME192324.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

DemodexMite Density Determinations by Standardized Skin Surface Biopsy and Direct Microscopic Examination and Their Relations with Clinical Types and Distribution Patterns

Yun

Yun²,

Baek

et al. 2017

Ann Dermatol

View full text Add to dashboard Cite

show abstract

“…In a recent study, it could be shown that the standardized skin surface biopsy is very effective in the detection of demodex mites compared to microscopic examination of secretions from sebaceous glands and might therefore be regarded as a reference standard for the detection of mites [28]. However, all of these (semi-)invasive tests have the disadvantage of a time delay before the result is obtained and cannot be repeated immediately if the result is negative whereas the detection of mites by HD-OCT is easily reproducible on different test sites and the result can be directly evaluated.…”

Section: Discussionmentioning

confidence: 99%

High-Definition Optical Coherence Tomography for the in vivo Detection of Demodex Mites

et al. 2012

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Background: Demodex mites are involved in different skin diseases and are commonly detected by skin scrape tests or superficial biopsies. A new high-definition optical coherence tomography (HD-OCT) with high lateral and axial resolution in a horizontal (en-face) and vertical (slice) imaging mode might offer the possibility of noninvasive and fast in vivo examination of demodex mites. Methods: Twenty patients with demodex-related skin diseases and 20 age- and gender-matched healthy controls were examined by HD-OCT. Mites per follicle and follicles per field of view were counted and compared to skin scrape tests. Results: HD-OCT images depicted mites in the en-face mode as bright round dots in groups of 3–5 mites per hair follicle. In the patients with demodex-related disease, a mean number of 3.4 mites per follicle were detected with a mean number of 2.9 infested follicles per area of view compared to a mean of 0.6 mites in 0.4 infested follicles in the controls. The skin scrape tests were negative in 21% of the patients. Conclusion: The innovative HD-OCT enables fast and noninvasive in vivo recognition of demodex mites and might become a useful tool in the diagnosis and treatment monitoring of demodex-related skin diseases.

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“…A standardized skin surface biopsy (SSSB) with a cyanoacrylic adhesive is the most recommended diagnostic tool for measuring the density of Demodex mites. Five or more mites counted on a surface area of 1 cm 2 on lesional skin is considered pathogenic (5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

The effects of sebum configuration on Demodex spp. density

et al. 2016

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Background/aim: Demodex spp. are ectoparasites living in the pilosebaceous units, which feed on the host's sebum and cellular proteins. The protective barrier of the skin consists of sebum secretion, moisture, and the acid mantle. In this study, we aimed to determine the effects of skin sebum, moisture, pH levels, and sebum configuration on Demodex spp. density Materials and methods: Forty-five patients who had demodicosis were enrolled in the study group, while the control group consisted of 40 subjects without demodicosis. Body fat percentage, serum triglyceride and cholesterol levels, skin sebum, moisture, and pH levels were measured. Demodex spp. density was determined with a standardized skin surface biopsy. Sebum samples were taken from the forehead and a high-performance thin-layer chromatography (HPTLC) method was performed on these samples. Subsequently, densitometric analyses were applied to the HPTLC plates.Results: Demodex spp. were found on the cheeks and lived in an alkali environment. Skin sebum and moisture levels were low in all groups. The skin pH levels and cholesterol ester in the sebum configuration were determined to be significantly higher in the group with demodicosis. Conclusion:We suggest that Demodex spp. may use cholesterol ester in the sebum as nutriment. In other words, cholesterol ester may be a suitable growth medium for the proliferation of Demodex spp.

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Comparison of the two techniques for measurement of the density of Demodex folliculorum : standardized skin surface biopsy and direct microscopic examination

Abstract: We recommend the use of SSSB for the measurement of Dd as more patients with demodicosis can be diagnosed with this method compared with the DME method.

Cited by 73 publications

References 12 publications

DemodexMite Density Determinations by Standardized Skin Surface Biopsy and Direct Microscopic Examination and Their Relations with Clinical Types and Distribution Patterns

DemodexMite Density Determinations by Standardized Skin Surface Biopsy and Direct Microscopic Examination and Their Relations with Clinical Types and Distribution Patterns

High-Definition Optical Coherence Tomography for the in vivo Detection of Demodex Mites

The effects of sebum configuration on Demodex spp. density

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