Comparison of the Transcriptomic and Epigenetic Profiles of Gonadal Primordial Germ Cells of White Leghorn and Green-Legged Partridgelike Chicken Embryos

Abstract

The Green-legged Partridgelike fowl is a native, dual-purpose Polish chicken. The White Leghorn has been intensively selected for several decades to mainly improve reproductive traits. Primordial germ cells (PGCs) represent the germline stem cells in chickens and are the only cells that can transfer the information stored in the genetic material from generation to generation. The aim of the study was to carry out a transcriptomic and an epigenetic comparison of the White Leghorn and Green-legged Partridgelike gonadal PGCs (gPGCs) at three developmental stages: days 4.5, 8, and 12 of the embryonic development. RNA and DNA were isolated from collected gPGCs. The RNA was further subjected to microarray analysis. An epigenetic analysis was performed based on the global methylation analysis and qMSP method for the particular silenced genes demonstrated in transcriptomic analysis. Statistically significant differences between the gPGCs from both breeds were detected on the day 8 of embryonic development. Global methylation analysis showed significant changes at the methylation level in the White Leghorn gPGCs on day 8 of embryonic development. The results suggest faster development of Green-legged Partridgelike embryos as compared to White Leghorn embryos. Changes in the levels of gene expression during embryonic development are determined by genetic and environmental factors, and this variability is influenced by breed and gender.

Epigenetic programming of chicken germ cells: a comparative review

“…These studies mainly focused on relationship between epigenetic regulation of economic trait, such as muscle content, immunity ( David et al, 2019 ; Willems et al, 2016 ), and further studies focusing on the effect of environment to the germ cell development remains to be explored. Dunislawska et al compared epigenetic regulation of PGCs between Green-legged Patridgelike fowl ( GP ) and White Leghorn and found faster development of GP embryo compared to that of White Leghorn and suggested that epigenetic mechanism of chicken embryo development depends on genetic background, environment, and gender ( Dunislawska et al, 2021 ). Therefore, when investigating epigenetic mechanism of chicken germ cells, external factors, including breeds, environmental factors, and genders, need to be considered.…”