Comparison of the survival of human biopsied embryos after cryopreservation with four different methods using non-transferable embryos

Zheng, W.T.; G, Zhuang; Zhou, Chao; Fang, Cong; Jiang, Ou; Li, T.; Zhang, M.F.; Liang, X.Y.

doi:10.1093/humrep/deh808

Cited by 79 publications

(54 citation statements)

References 21 publications

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“…Vitrification of human cleavage stage embryos versus slowrate freezing was evaluated in recent years by several investigators [5,7,8,32]. Zheng et al [32], in an experimental study, used abnormal biopsied 8-cell stage embryos and demonstrated significant improvement of embryo survival following vitrification (94%) compared with slow freezing (15%).…”

Section: Discussionmentioning

confidence: 99%

“…Until now, vitrification has been widely used for the cryopreservation of human oocytes [18,24,25], in vitro matured oocytes [26,27] pronuclear stage , cleavage stage [5,7,14,18,[29][30][31][32][33][34], or blastocyst-stage [8,18,[35][36][37][38][39][40][41][42]. However there are few publications that show clinical data on the basis of vitrification versus slow freezing, especially for the cleavage stage [5,8].…”

Section: Introductionmentioning

confidence: 99%

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Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Valojerdi

Eftekhari‐Yazdi

Karimian

et al. 2009

J Assist Reprod Genet

141

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Purpose The objective of this retrospective study was to evaluate the efficacy of vitrification and slow freezing for the cryopreservation of human cleavage stage embryos in terms of post-warming survival rate, post-warming embryo morphology and clinical outcomes. Methods The embryos of 305 patients at cleavage stages were cryopreserved either with vitrification (153 patients) or slow-freezing (152 patients) methods. After warming; the survival rate, post-warmed embryo morphology, clinical pregnancy and implantation rates were evaluated and compared between the two groups. Result(s) In the vitrification group versus slow freezing group, the survival rate (96.9% vs. 82.8%) and the postwarmed excellent morphology with all blastomeres intact (91.8% vs. 56.2%) were higher with an odds ratio of 6.607 (95% confidence interval; 4.184-10.434) and 8.769 (95% confidence interval; 6.460-11.904), respectively. In this group, the clinical pregnancy rate (40.5% vs. 21.4%) and the implantation rate (16.6% vs. 6.8%) were also higher with an odds ratio of 2.427 (95%confidence interval; 1.461-4.033) and 2.726 (95% confidence interval; 1.837-4.046), respectively. Conclusion(s) Vitrification in contrast to slow freezing is an efficient method for cryopreservation of human cleavage stage embryos. Vitrification provides a higher survival rate, minimal deleterious effects on post-warming embryo morphology and it can improve clinical outcomes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Valojerdi

Eftekhari‐Yazdi

Karimian

et al. 2009

J Assist Reprod Genet

141

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show abstract

“…Several recent studies have shown that human embryo survival at different stages after biopsy and conventional cryopreservation is extremely poor [2,3]. Vitrification has been proposed as an efficient method for vitrification of biopsied embryos, either at early cleavage stages [12] or at the blastocyst stage [9,10]. In the latest results reported by Zech, it would seem that the survival rate of ZP-opened blastocysts is related to the degree of expansion, with higher survival rates for expanded blastocysts.…”

Section: Discussionmentioning

confidence: 99%

Birth after transfer of frozen-thawed vitrified biopsied blastocysts

Parriego

Solé

Aurell

et al. 2007

J Assist Reprod Genet

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Purpose: To present a case describing the birth of a healthy female after the replacement of vitrified biopsied embryos after Preimplantation Genetic Diagnosis.Method: A descriptive case report of a single patient.Results: Our patient carrier of an X-linked disease became pregnant and as a result a healthy girl was born.Conclusions: This report shows that blastocysts obtained from biopsied embryos can be successfully cryopreserved by a simple, secure and low-cost vitrification method using a Hemi-straw support.

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“…The survival rate can, however, be improved by vitrification after incubating the cleavage-stage embryos for 6-8 h following biopsy (Zheng et al, 2005), or by cryopreservation in CJ3 medium (Stachecki et al, 2005). Recently higher survival rates of biopsied cleavage-stage embryos have been obtained by vitrification at the blastocyst stage (Magli et al, 2006;Zhang et al, 2009).…”

Section: Cryopreservation Of Biopsied Embryos At the Blastocyst Stagementioning

confidence: 99%

Preimplantation Genetic Testing: Current Status and Future Prospects

Lau¹,

Janson²,

Ball³

et al. 2012

In Vitro Fertilization - Innovative Clinical and Laboratory Aspects

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Comparison of the survival of human biopsied embryos after cryopreservation with four different methods using non-transferable embryos

Abstract: Non-transferable embryos derived from clinical IVF/ICSI are useful for evaluation of the optimal freezing procedures for biopsied embryos. Vitrification increases the survival rate of human biopsied embryos above standard and modified cryopreservation methods.

Cited by 79 publications

References 21 publications

Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Birth after transfer of frozen-thawed vitrified biopsied blastocysts

Preimplantation Genetic Testing: Current Status and Future Prospects

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