Comparison of the Structures of Triacylglycerols from Native and Transgenic Medium‐Chain Fatty Acid‐Enriched Rape Seed Oil by Liquid Chromatography–Atmospheric Pressure Chemical Ionization Ion‐Trap Mass Spectrometry (LC–APCI‐ITMS)

Overexpression of Cuphea viscosissima CvFatB4 enhances 16:0 fatty acid accumulation in Arabidopsis

“…Note that rather broad substrate use was observed in most cases and that fatty acids not indicated in the figure also showed higher accumulation levels compared to the control. NCBI accession numbers, organism(s) used in the heterologous enzyme expression analysis, and relevant references are as follows: 1 DQ238595 in Arabidopsis (Filichkin et al 2006); 2 GU225693 in E. coli K27 (Jing et al 2011); 3 U17076 in E. coli and rapeseed (Jones et al 1995); 4 U39834 in E. coli (Dehesh et al 1996 b ), Arabidopsis (Tjellström et al 2013), and rapeseed (Dehesh et al 1996 b ); 5 KC675178 in Arabidopsis (Tjellström et al 2013) and Camelina (Kim et al 2015); 6 U38188 in E. coli (Dehesh et al 1996 a ); 7 U56104 in Arabidopsis (Leonard et al 1997); 8 JF338906 in E. coli (Jing et al 2011) and Camelina (Kim et al 2015); 9 DQ238594 in Arabidopsis (Filichkin et al 2006); 10 AJ131741 in rapeseed (Beermann et al 2007); 11 JF338908 in E. coli (Jing et al 2011); 12 U38189 in E. coli and Arabidopsis (Tjellström et al 2013); 13 KC675179 in Camelina (Kim et al 2015); 14 U56103 in Arabidopsis (Leonard et al 1997); 15 and Q41635 in Arabidopsis (Voelker et al 1992) and rapeseed (Knutzon et al 1999). The scale bar shows the fractional differences in the amino acid sequences Palmitate (16:0) contents in seeds from the T 2 progenies The palmitate proportions in the seed oil were determined for T 2 progenies obtained from the self-pollination of six T 1 lines shown in Table 1.…”

“…They also have the potential to be hydrocarbon components in kerosene-type jet fuel along with the 16:0 fatty acid (palmitic acid) (Kim et al 2015). To date, dozens of acyl-ACP thioesterases, which were isolated from seeds that accumulate high levels of MCFAs, have been functionally characterized (Beermann et al 2007;Dehesh et al 1996a, b;Filichkin et al 2006;Jing et al 2011;Jones et al 1995;Kim et al 2015;Leonard et al 1997;Nam et al 2019;Tjellström et al 2013;Töpfer 1995;Voelker et al 1992). For example, UcFatB cDNA from Umbellularia californica (California bay) seeds has been shown to encode a 12:0-ACP thioesterase and produces lauric acid in the seeds when expressed in Arabidopsis thaliana (Arabidopsis) and Brassica napus (rapeseed) (Davies et al 199;Knutzon et al 1999;Pollard et al 1991;Voelker et al 1992).…”

“…Cuphea, a member of the Lythraceae, is a dicot genus of 260 species, whose seed oils contain high levels of MCFAs (Graham et al 1981). Substrate specificity or ranges of Cuphea seed acyl-ACP thioesterase were characterized using heterologous systems, such as E. coli, Arabidopsis, or rapeseed systems (Beermann et al 2007;Dehesh et al 1996a, b;Filichkin et al 2006;Jing et al 2011;Jones et al 1995;Kim et al 2015;Leonard et al 1997;Tjellström et al 2013). Cuphea viscosissima, known as blue waxweed, accumulates predominantly 8:0 and 10:0 fatty acids in its seed oil (Knapp and Tagliani 1991).…”