Comparison of the standard and reduced pH Syrian Hamster Embryo (SHE) cell in vitro transformation assays in predicting the carcinogenic potential of chemicals

Isfort, Robert J.; Kerckaert, Gary A.; LeBoeuf, Robert A.

doi:10.1016/0027-5107(95)00197-2

Cited by 75 publications

(22 citation statements)

References 159 publications

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“…In addition, the other 5 chemical agents that failed to induce chromosome aberrations were negative in the cell transformation assay, except for iodoform (5,10,23), indicating that the clastogenicity of these chemical agents correlates well with their transforming potential in SHE cells. Since the SHE cell transformation system has significant potential to predict the carcinogenicity of chemicals (28), the tested chemical agents with clastogenic activity are potentially carcinogenic. Other genotoxicity tests remain to be done to clarify the mutagenicity and carcinogenicity of these chemical agents used in dental practice.…”

Section: Discussionmentioning

confidence: 99%

Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells

et al. 2005

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Abstract. To assess the genotoxicity of 14 chemical agents used in dental practice, the ability of these agents to induce chromosome aberrations was examined using Syrian hamster embryo (SHE) cells. Statistically significant increases in the frequencies of chromosome aberrations were induced in SHE cells treated with 7 of 10 chemical agents used as endodontic medicaments, that is, carbol camphor, m-cresol, eugenol, guaiacol, zinc oxide, hydrogen peroxide, and formaldehyde. The other 3 chemical agents, that is, thymol, glutaraldehyde, and iodoform, did not increase the levels of chromosome aberrations. Of the 4 chemical agents that are used as an antiseptic on the oral mucosa, chromosome aberrations were induced by iodine, but not by the other 3 antiseptics, benzalkonium chloride, benzethonium chloride, and chlorhexidine. Among the 6 chemical agents exhibiting a negative response in the assay, only thymol induced chromosome aberrations in the presence of exogenous metabolic activation. Our results indicate that chemical agents having a positive response in the present study are potentially genotoxic to mammalian cells and need to be studied further in detail.

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Section: Discussionmentioning

confidence: 99%

Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells

et al. 2005

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“…Since the seminal work of Berwald and Sachs (1), Syrian hamster embryo (SHE) cells have been used to evaluate the potential of a wide variety of chemical and physical agents to induce morphological transformation (2). SHE cells are diploid, genetically stable, of finite lifespan, and capable of metabolizing many chemicals to their ultimate carcinogenic form (3).…”

Section: Introductionmentioning

confidence: 99%

Use of the Syrian hamster embryo cell transformation assay for carcinogenicity prediction of chemicals currently being tested by the National Toxicology Program in rodent bioassays.

Kerckaert

Brauninger

LeBoeuf

et al. 1996

Environ Health Perspect

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The Syrian hamster embryo (SHE) cell transformation assay was used to predict the carcinogenicity of 26 chemicals currently being tested in the rodent bioassay by the National Toxicology Program as part of its program titled "Strategies for Predicting Chemical Carcinogenesis in Rodents." Of these 26 chemicals, 17 were found to be positive in the SHE cell transformation assay while 9 were negative. Carcinogenicity predictions were made for these chemicals, based upon the SHE cell transformation assay results. Our predictions will be compared with the rodent bioassay results as they become available. Environ Health Perspect

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“…We measured cell division rates using bromodeoxyuridine (BrdU; Sigma Chemical) incorporation with PI staining (Sigma Chemical), which allows calculation of potential doubling time (T pot ), a measure of cell cycle time that takes growth fraction but not cell loss into account (10,11). The relationship between T pot and cell division rate (α) was defined as [4] We measured potential doubling times with flow cytometry by pulse-labeling cells with BrdU and detecting its incorporation into DNA by the use of fluorescent antibodies. The cells were then counterstained by PI to measure the total DNA content.…”

Section: Methodsmentioning

confidence: 99%

A Biologically Based Model of Growth and Senescence of Syrian Hamster Embryo (SHE) Cells after Exposure to Arsenic

Liao¹,

Gustafson²,

Fox³

et al. 2001

Environmental Health Perspectives

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Comparison of the standard and reduced pH Syrian Hamster Embryo (SHE) cell in vitro transformation assays in predicting the carcinogenic potential of chemicals

Cited by 75 publications

References 159 publications

Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells

Ability of Fourteen Chemical Agents Used in Dental Practice to Induce Chromosome Aberrations in Syrian Hamster Embryo Cells

Use of the Syrian hamster embryo cell transformation assay for carcinogenicity prediction of chemicals currently being tested by the National Toxicology Program in rodent bioassays.

A Biologically Based Model of Growth and Senescence of Syrian Hamster Embryo (SHE) Cells after Exposure to Arsenic

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