Comparison of the SSSperm testing kitwith the Halosperm testing kit in an analysis of sperm DNA fragmentation

Quyen, Le Thi; Thư, Nguyễn Thị Anh; Phuong, Le Thi Minh; Trang, Nguyen Thi

doi:10.31276/vjste.62(2).71-76

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“…6 . The number of samples was calculated based on the one-proportion estimation formula from Stanley Lemeshow et al with a consecutive sampling technique rounded up to 15 people (Quyen et al, 2020). The cement was collected in a sterile container and left at room temperature for 30 minutes for liquefaction.…”

Section: Bioactivity Of Uncaria Gambir (Whunter) Roxb On In-vitro Hum...mentioning

confidence: 99%

Bioactivity Examination of Uncaria gambir (W.Hunter) Roxb on In Vitro Human Sperm Motility

Permatasari,

Halisa,

Frethernety

2023

Med.Lab.Tech.J.

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Globally, 48 million couples experience infertility, where male infertility factors contribute to 50% of cases. Spermatozoa motility is a crucial parameter in assessing male fertility. Antioxidants act as the body's defence against excessive ROS and can be used as a treatment for male infertility. One of the local plants in Central Kalimantan that is potentially rich in antioxidants is Bajakah Kalalawit (Uncaria gambir (W.Hunter) Roxb. However, there is limited research on the bioactivity of Bajakah Kalalawit on spermatozoa in vitro. This study aims to determine the effect of ethanol extract of Uncaria gambir (W. Hunter) Roxb on spermatozoa motility in vitro. Bajakah Kalalawit stems were extracted using a 3x24-hour maceration technique with 96% ethanol. After obtaining the concentrated extract, the secondary metabolite compound content was analyzed through a phytochemical screening. The sample used was in vitro human spermatozoa that were washed and added to Bigger Whitten Whittingham medium, then incubated at 37°C for 1 hour with a 96% ethanol extract of Bajakah Kalalawit at doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml, as well as a control group (Bigger, Whitten & Whittingham medium only). The results showed that the compounds contained in the ethanol extract of Uncaria gambir (W.Hunter) Roxb were terpenoids, flavonoids, phenolics, steroids, saponins, alkaloids, and tannins. Spermatozoa motility significantly increased in the treatment groups starting from doses of 50ng/ml, 100ng/ml, 500ng/ml, and 1000ng/ml compared to the control group. Ethanol extract of Uncaria gambir (W.Hunter) Roxb could increase spermatozoa motility in vitro and succeeded in improving reproductive technology.

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Section: Bioactivity Of Uncaria Gambir (Whunter) Roxb On In-vitro Hum...mentioning

confidence: 99%

Bioactivity Examination of Uncaria gambir (W.Hunter) Roxb on In Vitro Human Sperm Motility

Permatasari,

Halisa,

Frethernety

2023

Med.Lab.Tech.J.

View full text Add to dashboard Cite

show abstract

Comparison of the SSSperm testing kitwith the Halosperm testing kit in an analysis of sperm DNA fragmentation

Cited by 1 publication

References 0 publications

Bioactivity Examination of Uncaria gambir (W.Hunter) Roxb on In Vitro Human Sperm Motility

Bioactivity Examination of Uncaria gambir (W.Hunter) Roxb on In Vitro Human Sperm Motility

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