Comparison of the Serum Neutralization Test and a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Vesicular Stomatitis Virus New Jersey and Vesicular Stomatitis Virus Indiana

Alvarado, Juan; Dolz, Gaby; Herrero, Marco V.; McCluskey, Brian J.; Salman, Mo

doi:10.1177/104063870201400309

Cited by 10 publications

(2 citation statements)

References 8 publications

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“…Receiver-operating characteristics (ROC) curves, the area under the curve (AUC), the determination of optimal cut-off point, the calculation of the positive and negative predictive values (PPV and NPV, respectively) and the agreement test (inter-rater agreement—κ coefficient) were conducted with the software MedCalc v15.2 for Windows (MedCalc Software). The κ coefficient was calculated to determine the magnitude of the statistical agreement between two tests [ 25 ].…”

Section: Methodsmentioning

confidence: 99%

Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5

et al. 2016

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Bovine herpesviruses 1 and 5 (BoHV-1 and BoHV-5) are economically important pathogens, associated with a variety of clinical syndromes, including respiratory and genital disease, reproductive failure and meningoencephalitis. The standard serological assay to diagnose BoHV-1 and BoHV-5 infections is the virus neutralization test (VNT), a time consuming procedure that requires manipulation of infectious virus. In the present study a highly sensitive and specific single dilution indirect ELISA was developed using recombinant glycoprotein D from BoHV-5 as antigen (rgD5ELISA). Bovine serum samples (n = 450) were screened by VNT against BoHV-5a and by rgD5ELISA. Compared with the VNT, the rgD5ELISA demonstrated accuracy of 99.8%, with 100% sensitivity, 96.7% specificity and coefficient of agreement between the tests of 0.954. The rgD5ELISA described here shows excellent agreement with the VNT and is shown to be a simple, convenient, specific and highly sensitive virus-free assay for detection of serum antibodies to BoHV-5.

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Section: Methodsmentioning

confidence: 99%

Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5

et al. 2016

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“…The SNT was carried out for these samples in a Six-Well Cell Culture Plates with duck embryo fibroblast cells. Duplicates of serial twofold dilutions of sera, inactivated at 56°C for 30 min were tested as previously described [54]. Titres were expressed as the reciprocal of the serum dilution that inhibited 75% of viral cytopathic effect.…”

Section: Methodsmentioning

confidence: 99%

A Thymidine Kinase recombinant protein-based ELISA for detecting antibodies to Duck Plague Virus

Wen

Cheng

Wang

et al. 2010

Virol J

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BackgroundDuck plague virus (DPV) is the causative agent of Duck Plague (DP) that causes significant morbidity and mortality throughout duck-producing areas of the world. The diagnosis of DP currently relies on the use of live or inactivated whole DPV virion as antigens in ELISA, but it is too laborious and expensive for routine application, and it is still difficult to get purified DPV virion with current technology.ResultsIn this study, we describe the expression and purification of a recombinant Thymidine Kinase (TK) protein which makes antigen in an in-house developed, optimized and standardized ELISA. The specificity of the optimized TK-ELISA was evaluated by antisera against Duck Plague Virus (DPV), Duck Hepatitis B Virus (DHBV), Duck Hepatitis Virus (DHV), Riemerella Anatipestifer(R. A), Escherichia coli (E. coli) and Salmonella anatum (S. anatum). Only antisera against DPV yielded a specific and strong signal. In order to determine the sensitivity of the TK-ELISA, a panel of diluted sera was tested, and the minimum detection limit of 1:2560 (OD450 nm = 0.401) was obtained according to the endpoint cut-off (0.2438). The repeatability and reproducibility under the experimental conditions demonstrates a low variability (P > 0.05). The suspected sera samples (n = 30) were determined by TK-ELISA and the positive rate is 90% (27/30), and the TK-ELISA showed 83.33% (22+3/30) coincidence rate with the Serum Neutralization Test (SNT) and 90% (24+3/30) coincidence rate with the whole DPV virion based-ELISA (DPV-ELISA). When defining the dynamics of antibody response to attenuated live DPV vaccine, the maximum antibodies is reached after 4 weeks.ConclusionsThe results suggest that the TK-ELISA provides high specificity, sensitivity, repeatability and reproducibility for detection of anti-DPV antibodies in duck sera, and has the potential to be much simpler than DPV-ELISA and SNT for the sera epidemiological investigation.

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Vesicular Stomatitis

McCluskey¹

2007

Equine Infectious Diseases

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Comparison of the Serum Neutralization Test and a Competitive Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Vesicular Stomatitis Virus New Jersey and Vesicular Stomatitis Virus Indiana

Cited by 10 publications

References 8 publications

Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5

Development of an Indirect ELISA for Serological Diagnosis of Bovine herpesvirus 5

A Thymidine Kinase recombinant protein-based ELISA for detecting antibodies to Duck Plague Virus

Vesicular Stomatitis

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