Comparison of the sensitivity of evaporative universal detectors and LC/MS in the HILIC and the reversed-phase HPLC modes

Mitchell, Clifford R.; Yu, Bo; Benz, Nancy J.; Zhang, Shuhong

doi:10.1016/j.jchromb.2009.10.027

Cited by 76 publications

(38 citation statements)

References 25 publications

(30 reference statements)

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“…Reversed phase is a successful mode for a wide range of hydrophilic, hydrophobic, and ionizable compounds; on the other hand, poor retention occurs with unsuccessful peaks for separation of polar molecules [28,29]. Polar compounds are generally separated with normal-phase liquid chromatography or hydrophilic interaction chromatography (HILIC) mode.…”

Section: Chromatographymentioning

confidence: 99%

A simple synthetic route for the preparation of a reversed-phase stationary phase based on monosized-porous hydrogel beads and its chromatographic use for separation of small molecules

Çelebі

2017

Acta Chromatographica

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Summary. New polyacrylate-based monosized-porous polymer beads were proposed as a stationary phase for the separation of polar compounds by microbore reversed-phase chromatography. For this purpose, monosized-porous poly(glycerol dimethacrylate-coglycerol-1,3-diglycerolate diacrylate), poly(GDMA-co-GDGDA), beads with hydroxyl functionality were synthesized by a modified seeded polymerization. The selected octadecylating agent, stearoyl chloride (SC), was covalently attached onto the hydrophilic beads via a direct, single stage reaction with a simple synthetic route. SC attachedpoly(GDMA-co-GDGDA) beads were slurry-packed into the microbore columns and used as separation medium microbore reversed-phase chromatography. The stationary phase was used for separation of alkylbenzenes and polar analytes by micro reversedphase chromatography, using mobile phases with low acetonitrile content. Theoretical plate number (TPN) values up to 12,000 plates m −1 and 10,000 plates m −1 for alkylbenzenes and polar analytes, respectively, were achieved. The results also showed that poly(GDMA-co-GDGDA) hydrogel beads are a promising starting material for a number of chromatographic applications like reversed-phase (RP) chromatography, hydrophilic interaction chromatography (HILIC), ion-exchange chromatography (IEC), and affinity chromatography with a single-stage surface modification.

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Section: Chromatographymentioning

confidence: 99%

A simple synthetic route for the preparation of a reversed-phase stationary phase based on monosized-porous hydrogel beads and its chromatographic use for separation of small molecules

Çelebі

2017

Acta Chromatographica

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“…Temperatures as high as 200°C have been explored 49 . Temperature programming has been utilised to enhance resolution of poorly resolving analytes 50 .…”

Section: Hydrophilicmentioning

confidence: 99%

Untitled

2017

IJPSR

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Impurities will be present in all drug substances and drug products, i.e. nothing is 100% pure if one looks in enough depth. The current regulatory guidance on impurities accepts this, and for drug products with a dose of less than 2 g/day identification of impurities is set at 0.1% levels and above (ICH Q3B (R2), 2006). For some impurities, this is a simple undertaking as generally available analytical techniques can address the prevailing analytical challenges; whereas, for others this may be much more challenging requiring more sophisticated analytical approaches. The present review provides an insight into current development of analytical techniques to investigate and quantify impurities in drug substances and drug products providing discussion of progress particular within the field of chromatography to ensure separation of and quantification of those related impurities. Further, a section is devoted to the identification of classical impurities, but in addition, inorganic (metal residues) and solid state impurities are also discussed. Risk control strategies for pharmaceutical impurities aligned with several of the ICH guidelines, are also discussed.

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“…The increase in the sensitivity using the HILIC column appeared to be due to an enhancement of the ionization efficiency of adenosine in an eluent containing a high concentration of acetonitrile. 18,19 It was thus found that separation on an HILIC column is an effective means to improve the sensitivity for the ESI-MS/MS detection of adenosine in human plasma.…”

Section: Effect Of Ultrafiltration and Hilic Separation Upon Esi Of Amentioning

confidence: 99%

Simple Pretreatment and HILIC Separation for LC-ESI-MS/MS Determination of Adenosine in Human Plasma

et al. 2015

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A simple pretreatment method and separation mode for the LC-ESI-MS/MS determination of adenosine in human plasma have been developed. Deproteinization by acetonitrile and ultrafiltration followed by chromatographic separation with a hydrophilic interaction chromatographic (HILIC) column give a highly sensitive MS/MS response without ionic suppression caused by the matrix compounds in human plasma. In addition, the presence of ammonium acetate in the mobile phase contributes to high sensitivity in MS/MS detection, facilitating the ionization of adenosine. This method seems to be amenable to the treatment of many samples in clinical practice.

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Comparison of the sensitivity of evaporative universal detectors and LC/MS in the HILIC and the reversed-phase HPLC modes

Cited by 76 publications

References 25 publications

A simple synthetic route for the preparation of a reversed-phase stationary phase based on monosized-porous hydrogel beads and its chromatographic use for separation of small molecules

A simple synthetic route for the preparation of a reversed-phase stationary phase based on monosized-porous hydrogel beads and its chromatographic use for separation of small molecules

Untitled

Simple Pretreatment and HILIC Separation for LC-ESI-MS/MS Determination of Adenosine in Human Plasma

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