Comparison of the Sensititre YeastOne colorimetric antifungal panel and Etest with the NCCLS M38-A method to determine the activity of amphotericin B and itraconazole against clinical isolates of Aspergillus spp.

Martín-Mazuelos, Estrella; Pemán, Javier; Valverde, Anastasio; Chaves, Mônica; Serrano, M. C. López; Cantón, Emilia

doi:10.1093/jac/dkg384

Cited by 62 publications

(59 citation statements)

References 20 publications

(16 reference statements)

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“…Practically no differences were observed among both microdilution methods; by the Sensititre method, all the strains were inhibited by 1 mg of drug/liter. This result is in concordance with previous studies using Sensititre with another azole antifungal agent, itraconazole (10,14).…”

supporting

confidence: 93%

“…Overall, higher voriconazole MICs were obtained by the Sensititre method; similar results were obtained by Martín-Mazuelos et al (10) and Sánchez Sousa et al (14) with itraconazole. In contrast, Meletiadis et al (11) found that itraconazole MICs generated by Sensititre YeastOne were lower than those generated by the NCCLS method.…”

supporting

confidence: 87%

“…For itraconazole, Meletiadis et al (11) found that the level of agreement between the methods was greater after 48 h of incubation (84.6%) than that after 24 h of incubation (44.6%). Martín-Mazuelos et al (10) also found the best agreement after 48 h of incubation for this antifungal agent (90.2%). In contrast, Sánchez Sousa et al (14) found better agreement when colorimetric results after 24 h of incubation were compared with reference broth microdilution MICs after 48 h of incubation.…”

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confidence: 73%

“…Martín-Mazuelos et al (10), testing with itraconazole, also found the best agreement for A. niger isolates, followed by A. fumigatus and A. flavus isolates. Discrepancies (greater than Ϯ2 dilutions) between the MICs determined by the Sensititre and NCCLS M38-A methods were demonstrated for nine isolates (14.28%) tested against voriconazole: three A. fumigatus isolates, three A. flavus isolates, two A. glaucus isolates, and one A. terreus isolate.…”

mentioning

confidence: 87%

“…Alternatives to the NCCLS method are currently under investigation, including two commercial methods, E-test and Sensititre YeastOne, which have been evaluated for yeast and molds (1,3,5,6,9,10,11,(13)(14)(15)(16)(17).…”

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confidence: 99%

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Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with a Modified NCCLS M38-A Method To Determine the Activity of Voriconazole against Clinical Isolates of Aspergillus spp

et al. 2004

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The susceptibilities of 63 isolates of Aspergillus spp. to voriconazole were evaluated by a modified NCCLS M38-A method and the Sensititre YeastOne method. The overall agreement was 82.5%, ranging from 100% for Aspergillus niger and Aspergillus terreus to 62.5% for Aspergillus flavus. Discrepancies between the methods were due to higher Sensititre MICs. The Sensititre YeastOne method could have potential value for susceptibility testing of Aspergillus spp. to voriconazole.Over the past decades, the incidence of invasive fungal infections has increased, especially those caused by Aspergillus species (18). The treatment of choice for infected patients remains amphotericin B, although alternative drugs with activities against Aspergillus species are becoming available for clinical use, including antifungal azoles and echinocandins (6,18). Because the number of serious infections caused by Aspergillus spp. has increased, as has the resistance of Aspergillus spp. to established agents, determination of the in vitro susceptibilities of Aspergillus isolates to both established and investigational agents is necessary (6).There is a great need for an easier and reproducible method for in vitro susceptibility testing of filamentous fungi as a guide to selecting and monitoring antifungal therapy. Alternatives to the NCCLS method are currently under investigation, including two commercial methods, E-test and Sensititre YeastOne, which have been evaluated for yeast and molds (1,3,5,6,9,10,11,(13)(14)(15)(16)(17).Our study represents an evaluation of the suitability of Sensititre YeastOne for determining the susceptibilities of Aspergillus spp. isolates to voriconazole. This test is a commercial colorimetric panel that consists of a disposable tray which contains dried serial dilutions of five antifungal agents in individual wells. The wells also contain an oxidation-reduction indicator (Alamar Blue) to generate clear-cut endpoints based on a visually detectable color change. MICs obtained by this method were compared to those obtained by the modified reference broth microdilution method (14).A total of 63 clinical Aspergillus isolates, comprising 24 Aspergillus fumigatus isolates, 16 Aspergillus flavus isolates, 9 Aspergillus terreus isolates, 8 Aspergillus niger isolates, 4 Aspergillus glaucus isolates, and 2 Aspergillus nidulans isolates, were included in this study. These isolates were recovered from clinical specimens of patients at the Valme University Hospital, Seville, Spain, and the Division of Infectious Diseases, Medical College of Virginia, Virginia Commonwealth University, Richmond.Culture, identification, and preservation of strains were done by using routine mycological methods (4, 7). Candida krusei ATCC 6258 and Candida parapsilosis ATCC 22019 were used as quality control strains for susceptibility testing procedures, and Aspergillus fumigatus ATCC 204305 and Aspergillus flavus ATCC 204304 were used as reference strains.The broth microdilution method was performed according to modified NCCLS standard M38-A gu...

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supporting

confidence: 93%

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confidence: 87%

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confidence: 73%

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confidence: 87%