Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst

Czobor, Ádám; Hajdinák, Péter; Németh, Bence; Piros, Borbála; Németh, Áron; Szarka, András

doi:10.1371/journal.pone.0210592

Cited by 8 publications

(3 citation statements)

References 89 publications

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“…These results are in accordance with previous reports in which application of elicitor harpin (virulence factors produced by bacterial pathogens such as P. syringae) inhibited mitochondrial respiration and causing a strong induction of AOX in Arabidopsis cells (Krause and Durner, 2004). Several studies have described AOX1a as the most stress-responsive AOX gene, whereas AOX1c expression is relatively stable or even decreased in response to stresses or elicitor treatment (Clifton et al, 2006;Czobor et al, 2019).…”

Section: Discussionsupporting

confidence: 93%

An Essential Role of Mitochondrial α-Ketoglutarate Dehydrogenase E2 in the Basal Immune Response Against Bacterial Pathogens in Tomato

Liu

Fang

et al. 2020

Front. Plant Sci.

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Plants intensely modulate respiration when pathogens attack, but the function of mitochondrial respiration-related genes in plant-bacteria interaction is largely unclear.Here, the functions of α-ketoglutarate dehydrogenase (α-kGDH) E2 subunit and alternative oxidase (AOX) were investigated in the interaction between tomato and the virulent bacterial pathogen Pseudomonas syringae pv. tomato DC3000 (Pst). Pst inoculation suppressed the transcript abundance of α-kGDH E2, but enhanced AOX expression and salicylic acid (SA) accumulation. Gene silencing and transient overexpression approaches revealed that plant susceptibility to Pst was significantly reduced by silencing α-kGDH E2 in tomato, but increased by overexpressing α-kGDH E2 in Nicotiana benthamiana, whereas silencing or overexpressing of AOX1a did not affect plant defense. Moreover, silencing octanoyltransferase (LIP2), engaged in the lipoylation of α-kGDH E2, significantly reduced disease susceptibility and hydrogen peroxide accumulation. Use of transgenic NahG tomato plants that cannot accumulate SA as well as the exogenous SA application experiment evidenced that α-kGDH E2 acts downstream of SA defense pathway. These results demonstrate tomato α-kGDH E2 plays a negative role in plant basal defense against Pst in an AOX-independent pathway but was associated with lipoylation and SA defense pathways. The findings help to elucidate the mechanisms of mitochondria-involved plant basal immunity.

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Section: Discussionsupporting

confidence: 93%

An Essential Role of Mitochondrial α-Ketoglutarate Dehydrogenase E2 in the Basal Immune Response Against Bacterial Pathogens in Tomato

Liu

Fang

et al. 2020

Front. Plant Sci.

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“…In addition to AOX, the plant mitochondrial inner membrane possesses UCPs. The UCPs belong to the superfamily of mitochondrial carrier proteins dissipating the proton electrochemical gradient generated by the respiratory chain complexes [20]. In plants, these proteins are involved in mitochondrial energy flow regulation.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analyses in a Selected Gene Set Indicate Alternative Oxidase (AOX) and Early Enhanced Fermentation as Critical for Salinity Tolerance in Rice

Aziz

Germano

Thiers

et al. 2022

Plants

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Plants subjected to stress need to respond rapidly and efficiently to acclimatize and survive. In this paper, we investigated a selected gene set potentially involved in early cell reprogramming in two rice genotypes with contrasting salinity tolerance (Pokkali tolerant and IR29 susceptible) in order to advance knowledge of early molecular mechanisms of rice in dealing with salt stress. Selected genes were evaluated in available transcriptomic data over a short period of 24 h and involved enzymes that avoid ROS formation (AOX, UCP and PTOX), impact ATP production (PFK, ADH and COX) or relate to the antioxidant system. Higher transcript accumulation of AOX (ROS balancing), PFK and ADH (alcohol fermentation) was detected in the tolerant genotype, while the sensitive genotype revealed higher UCP and PTOX transcript levels, indicating a predominant role for early transcription of AOX and fermentation in conferring salt stress tolerance to rice. Antioxidant gene analyses supported higher oxidative stress in IR29, with transcript increases of cytosolic CAT and SOD from all cell compartments (cytoplasm, peroxisome, chloroplast and mitochondria). In contrast, Pokkali increased mRNA levels from the AsA-GSH cycle as cytosolic/mitochondrial DHAR was involved in ascorbate recovery. In addition, these responses occurred from 2 h in IR29 and 10 h in Pokkali, indicating early but ineffective antioxidant activity in the susceptible genotype. Overall, our data suggest that AOX and ADH can play a critical role during early cell reprogramming for improving salt stress tolerance by efficiently controlling ROS formation in mitochondria. We discuss our results in relation to gene engineering and editing approaches to develop salinity-tolerant crops.

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“…Plants in their natural environments are exposed to a variety of biotic and abiotic stresses, including pathogens, drought, heavy metals, extreme temperature, salt and high light. Under these stress conditions, reactive oxygen species (ROS) derived from molecular oxygen can accumulate in plant cells [1–3]. Excess amount of ROS formation can lead to programmed cell death (PCD), moreover ROS are important factors in this process [4].…”

Section: Introductionmentioning

confidence: 99%

The potential role of acrolein in plant ferroptosis-like cell death

2019

Self Cite

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The iron dependent, programmed cell death, ferroptosis was described first in tumour cells. It showed distinct features from the already known cell death forms such as apoptosis, necrosis and autophagy. The caspase independent cell death could be induced by the depletion of glutathione by erastin or by the inhibition of the lipid peroxide scavenger enzyme GPX4 by RSL3 and it was accompanied by the generation of lipid reactive oxygen species. Recently, ferroptosis-like cell death associated to glutathione depletion, lipid peroxidation and iron dependency could also be induced in plant cells by heat treatment. Unfortunately, the mediators and elements of the ferroptotic pathway have not been described yet. Our present results on Arabidopsis thaliana cell cultures suggest that acrolein, a lipid peroxide-derived reactive carbonyl species, is involved in plant ferroptosis-like cell death. The acrolein induced cell death could be mitigated by the known ferroptosis inhibitors such as Ferrostatin-1, Deferoxamine, α-Tocopherol, and glutathione. At the same time acrolein can be a mediator of ferroptosis-like cell death in plant cells since the known ferroptosis inducer RSL3 induced cell death could be mitigated by the acrolein scavenger carnosine. Finally, on the contrary to the caspase independent ferroptosis in human cells, we found that caspase-like activity can be involved in plant ferroptosis-like cell death.

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Comparison of the response of alternative oxidase and uncoupling proteins to bacterial elicitor induced oxidative burst

Cited by 8 publications

References 89 publications

An Essential Role of Mitochondrial α-Ketoglutarate Dehydrogenase E2 in the Basal Immune Response Against Bacterial Pathogens in Tomato

An Essential Role of Mitochondrial α-Ketoglutarate Dehydrogenase E2 in the Basal Immune Response Against Bacterial Pathogens in Tomato

Transcriptome Analyses in a Selected Gene Set Indicate Alternative Oxidase (AOX) and Early Enhanced Fermentation as Critical for Salinity Tolerance in Rice

The potential role of acrolein in plant ferroptosis-like cell death

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