Comparison of the reactions of the compact-colony forming active substance (CCFAS) to the clumping-factor reaction in a strain ofStaphylococcus aureus with animal plasma

Abstract

Plasma was used from rat, mouse, guinea pig, rabbit, dog, cat, sheep, goat, monkey, horse, pig, cow, fox, mink, porpoise, deer, manatee, seal, elephant, raccoon, pigeon, macaw, and humans; clotting time and gel formation activities by the compact-colony forming active substance (CCFAS) extracted from a strain of Staphylococcus aureus and relative staphylococcal clumping factor reaction were determined. Experimental results showed that every plasma was clotted and gel formation of plasma was observed by the CCFAS, however, although it was shown by the other plasmas, no clumping-factor reaction was observed with plasma from guinea pig, goat, and elephant.

Comparison of compact colony-forming activity and paracoagulation activity of strains ofStaphylococcus aureus in serum and plasmas of various animals

“…We reported that CCFAS isolated from an unencapsulated strain of S. aureus, which forms compact colonies in serum-containing SSA, shows PC to cause direct conversion from fibrinogen to fibrin (Yoshida et al 1977(Yoshida et al , 1978Ohtomo et al 1980a;Usui et al 1982). This CCFAS, which is an alkali-stable polysaccharide, was also studied to clarify its specific bonding to fibrinogen Ohtomo et al 1985).…”

“…To calculate the dotting activity of plasma from various animal species by the CCFAS 1 ml of 0.03 M Tris-HC1 buffer, pH 8.4, containing 1.0 mg purified CCFAS was combined with the same volume of various undiluted animal plasmas, and the mixture was incubated at 37 °C for 0-15 min. To evaluate PC activity of plasma by CCFAS the turbidity change at 15 min after combination was measured spectrophotometrically at 370 nm according to the methods of Rampling et al (1976) and Ohtomo et al (1980a). In this experiment, each plasma specimen was tested four times and mean values and standard error values were obtained.…”