Comparison of the proportions of oral bacterial species in BALB/c mice from different suppliers

Rodrigue, L.; Lavoie, Marie

doi:10.1258/002367796780865853

Cited by 15 publications

(12 citation statements)

References 21 publications

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“…Our results are thus consistent with previous observations in which streptococci represent predominant commensals of the oral microbiota in humans and in laboratory mice [45, 49]. Moreover, as in prior studies that characterized the oral microbiota of mice from cultivatable bacteria [44] or from pyrosequencing [45] we detected a low number of species compared to humans, likely influenced by modern vivarium procedures to establish disease-free colonies. Interestingly, we find higher recoveries from Car6 −/− versus Car6 +/+ mice of L. murinus and of the non-hemolytic species putatively assigned as streptococci, supporting our hypothesis that CA VI expression influences the commensal oral microbiota.…”

Section: Discussionsupporting

confidence: 92%

“…For each colony morphotype, the highest scoring aligned sequence from the RDP database is associated with mice. Actinobacillus muris [43], L. murinus [44] and Staphylococcus saprophyticus [45] are murine commensal oral bacteria. Corynebacteria are commensals of the skin and mucous membranes of humans and animals, with C. mastitidis recently identified as an opportunistic infectious resident of the epidermis of laboratory mice [46].…”

Section: Discussionmentioning

confidence: 99%

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Oral colonization by Streptococcus mutans and caries development is reduced upon deletion of carbonic anhydrase VI expression in saliva

Culp

Robinson

Parkkila

et al. 2011

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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Carbonic anhydrase VI (CA VI), encoded by type A transcripts of the gene Car6, is a secretory product of salivary glands and is found in the enamel pellicle. Because higher caries prevalence is associated with lower salivary concentrations of CA VI in humans, we tested whether CA VI protects enamel surfaces from caries induced by Streptococcus mutans, using Car6−/− mice, in which salivary CA VI expression is absent. We detected aberrant Car6 type A transcripts in Car6−/− mice, likely targets for nonsense-mediated mRNA decay. Expression of the intracellular stress-induced isoform of CA VI encoded by type B transcripts was restricted to parotid and submandibular glands of wild type mice. The salivary function of Car6−/− mice was normal as assessed by the histology and protein/glycoprotein profiles of glands, salivary flow rates and protein/glycoprotein compositions of saliva. Surprisingly, total smooth surface caries and sulcal caries in Car6−/− mice were more than 6-fold and 2-fold lower than in wild type mice after infection with S. mutans strain UA159. Recoveries of S. mutans and total microbiota from molars were also lower in Car6−/− mice. To explore possible mechanisms for increased caries susceptibility, we found no differences in S. mutans adherence to salivary pellicles, in vitro. Interestingly, higher levels of Lactobacillus murinus and an unidentified Streptococcus species were cultivated from the oral microbiota of Car6−/− mice. Collective results suggest salivary CA VI may promote caries by modulating the oral microbiota to favor S. mutans colonization and/or by the enzymatic production of acid within plaque.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Oral colonization by Streptococcus mutans and caries development is reduced upon deletion of carbonic anhydrase VI expression in saliva

Culp

Robinson

Parkkila

et al. 2011

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Diseas

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“…were most frequently cultivated from the corresponding intestinal tracts. These results correlated well with previous cultivation-based microbial community analyses of the GI tract-associated microbiota of mice [4, 34]. …”

Section: Discussionsupporting

confidence: 90%

In Vitro Communities Derived from Oral and Gut Microbial Floras Inhibit the Growth of Bacteria of Foreign Origins

He¹,

Tian²,

Guo

et al. 2010

Microb Ecol

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The gastrointestinal (GI) tract is home to trillions of microbes. Within the same GI tract, substantial differences in the bacterial species that inhabit the oral cavity and intestinal tract have been noted. While the influence of host environments and nutritional availability in shaping different microbial communities is widely accepted, we hypothesize that the existing microbial flora also plays a role in selecting the bacterial species that are being integrated into the community. In this study, we used cultivable microbial communities isolated from different parts of the GI tract of mice (oral cavity and intestines) as a model system to examine this hypothesis. Microbes from these two areas were harvested and cultured using the same nutritional conditions, which led to two distinct microbial communities, each with about 20 different species as revealed by PCR-based denaturing gradient gel electrophoresis analysis. In vitro community competition assays showed that the two microbial floras exhibited antagonistic interactions toward each other. More interestingly, all the original isolates tested and their closely related species displayed striking community preferences: They persisted when introduced into the bacterial community of the same origin, while their viable count declined more than three orders of magnitude after 4 days of coincubation with the microbial flora of foreign origin. These results suggest that an existing microbial community might impose a selective pressure on incoming foreign bacterial species independent of host selection. The observed inter-flora interactions could contribute to the protective effect of established microbial communities against the integration of foreign bacteria to maintain the stability of the existing communities.Electronic supplementary materialThe online version of this article (doi:10.1007/s00248-010-9711-9) contains supplementary material, which is available to authorized users.

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“…Thus, the microbial community of WT2 may represent that of the mice from Charles River without the dominant Streptococcus species. The effect of the housing environment and the suppliers on the composition of mouse oral microbiota has been previously reported [16,17]. …”

Section: Resultsmentioning

confidence: 99%

The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer

et al. 2010

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BackgroundAlthough mice have long served as an animal model for periodontitis, information on the composition of their indigenous oral microbiota is limited. The aim of the current study was to characterize mouse oral bacterial flora by applying extensive parallel pyrosequencing using the latest model pyrosequencer, a Roche/454 Genome Sequencer FLX Titanium. In addition, the effect of Toll-like receptor (TLR) 2 deficiency on oral microbiota was evaluated.ResultsEight oral bacterial communities of wild-type (n = 4) and TLR2 knock-out (n = 4) C57BL/6 mice were characterized by analyzing 80,046 reads of 16S rRNA genes obtained by pyrosequencing. Excluding the PCR primers, the average length of each sequencing product was 443 bp. The average species richness of the murine oral bacterial communities was estimated to be about 200, but the communities were dominated by only two main phyla and several species. Therefore, the bacterial communities were relatively simple. The bacterial composition of the murine oral microbiota was significantly different from that of humans, and the lack of TLR2 had a negligible effect on the murine oral microbiota.ConclusionPyrosequencing using the Roche/454 FLX Titanium successfully characterized mouse oral bacterial communities. The relatively simple oral bacterial communities of mice were not affected by TLR2 deficiency. These findings will provide a basis for future studies on the role of periodontal pathogens in the murine model of periodontitis.

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Comparison of the proportions of oral bacterial species in BALB/c mice from different suppliers

Cited by 15 publications

References 21 publications

Oral colonization by Streptococcus mutans and caries development is reduced upon deletion of carbonic anhydrase VI expression in saliva

Oral colonization by Streptococcus mutans and caries development is reduced upon deletion of carbonic anhydrase VI expression in saliva

In Vitro Communities Derived from Oral and Gut Microbial Floras Inhibit the Growth of Bacteria of Foreign Origins

The analysis of oral microbial communities of wild-type and toll-like receptor 2-deficient mice using a 454 GS FLX Titanium pyrosequencer

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