Comparison of the Properties of Lipase Immobilized onto Mesoporous Resins by Different Methods

Abstract

Genipin, a natural cross-linking agent, was used for the immobilization of lipase from Candida sp. 99-125 by cross-linking to two kinds of mesoporous resins. Under optimum conditions, the activity recovery of immobilized lipase on resin NKA-9 could reach up to 96.99% when the genipin concentration was 0.5%, and it could reach up to 86.18% for S-8 with a genipin concentration of 0.25%. Compared with using glutaraldehyde as a cross-linking agent, the immobilized lipase using genipin showed better pH and thermal stability, storage stability, and reusability. The residual activity of immobilized lipase using genipin as cross-linker remained more than 60% of its initial activity after six hydrolytic cycles, whereas only about 35% activity remained by using glutaraldehyde as cross-linker.

Immobilization ofCandida rugosalipase by adsorption-crosslinking onto corn husk

“…After the crosslinking process through the addition of glutaraldehyde precisely causes a decrease in the value of C. rugosa lipase activity for all lipase concentration variations ( Figure 3). This is probably caused by the reaction conditions that are inconsistent with glutaraldehyde concentration so that the crosslinking process is not been successful [11]. The biggest activity value is the optimum condition because it shows the number of enzymes adsorbed on the support of corn husk is increasing [8] For reactions which lasted 4 hours, the addition of glutaraldehyde led to an increase in value of the activity of 1.2 and 2.37 U / g support respectively at lipase concentrations of 0.5 and 0.75 mg / ml (figure 4).…”

“…Adsorption method is a method of enzyme immobilization as it is the most simple, easy and economical. A disadvantage of adsorption immobilization method is the weak interaction between the enzyme and the support that results in low activity and stability [11]. The use of glutaraldehyde as a cross linker is one way to improve the interaction between the enzyme and support.…”

Effect of Anion and Amino Functional Group on Resin for Lipase Immobilization with Adsorption-Cross Linking Method

“…Based on Wang et al [2] the demand of enzymes is increasing every year especially for biofuel production.…”

“…The conventional biodiesel technology is usually catalyzed by inorganic base or acid, which makes the separation of catalyst from products have certain drawbacks such as more complex process and higher energy consumption, therefore consequently increases the cost of production [1], [2]. Lipase is an important enzyme that can be used not only for biodiesel production but also in reactions such as hydrolysis, esterification, transesterification, and interesterification.…”

“…Cross linking between lipase and support will maintain the activity and stability of enzyme during reaction. Enzyme immobilization on resin with adsorption-cross linking method has been done on mesoporous resin [2]. Previous studies show that lipase immobilization on NW-ZT2 with adsorption-cross linking method gives high enzyme loading and high stabillity [4].…”