Comparison of the Properties of Human Hemoglobin Covalently Bound to Carboxyl Dextrans with Free and Polymerized Hemoglobin

Pg, Pietta; Pace, Mario; Palazzini, Gabriele; Agostoni, A

doi:10.1080/10826068408070638

Cited by 3 publications

(2 citation statements)

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“…Amidation of oxy- and deoxyHb was achieved with activated carboxyl-derivatized dextrans . Thus, dextran derivatized with 6-bromohexanoic acid, 4.89 , was allowed to react with Hb in the presence of EDCI, yielding the conjugate 4.90 (Scheme ).…”

Section: Conjugation To Polysaccharides and Proteinsmentioning

confidence: 99%

“…Amidation of oxy-and deoxyHb was achieved with activated carboxyl-derivatized dextrans. 825 Thus, dextran derivatized with 6-bromohexanoic acid, 4.89, was allowed to react with Hb in the presence of EDCI, yielding the conjugate 4.90 (Scheme 9). Alternatively, the acid was treated with N-hydroxysuccinimide and dicyclohexylcarbodiimide (DCC) and the resulting dextran hydroxysuccinimide ester 4.91 was reacted with Hb.…”

Section: Conjugation To Polysaccharides and Proteinsmentioning

confidence: 99%

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Oxygen Carriers (“Blood Substitutes”)Raison d'Etre, Chemistry, and Some PhysiologyBlut ist ein ganz besondrer Saft

2001

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Pharmaceutical Corporation in San Diego; however, the opinion expressed here are solely his and not necessarily those of the Corporation. Jean G. Riess received his chemical engineer and doctorat-e `s-sciences degrees (the latter with Professor Guy Ourisson in 1963) from the University of Strasbourg. He then spent two years with John Van Wazer at Monsanto in Saint-Louis, MO, learning some phosphorus and transitionmetal chemistry. In 1968 he became Professor at the University of Nice, France, where he founded, directed, and eventually became honorary director of the Unite ´de Chimie Mole ´culaire (associated with the Centre National de la Recherche Scientifique). His research successively involved phosphorus chemistry, transition-metal chemistry, organometallics, and eventually perfluorochemicals. His present interests are in fluorocarbons, fluorinated amphiphiles, and their colloid chemistry, including fluorocarbon emulsions for in vivo O 2 delivery, fluorinated self-assemblies, and drug delivery systems. Professor Riess has published about 360 papers and holds ca. 25 patents. He has served on numerous councils and committees and has chaired or co-chaired international conferences on phosphorus chemistry and blood substitutes. He has won awards from the French Academy of Sciences, French Chemical Society, Alexander von Humboldt Stifftung, City of Nice and Controlled Release Society, as well as Alliance's first Distinguished Contribution Award. He holds an honorary Research Associate position at the University of California at San Diego and sits on the Board of Directors of Alliance Pharmaceutical Corp.

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Section: Conjugation To Polysaccharides and Proteinsmentioning

confidence: 99%

Section: Conjugation To Polysaccharides and Proteinsmentioning

confidence: 99%

Oxygen Carriers (“Blood Substitutes”)Raison d'Etre, Chemistry, and Some PhysiologyBlut ist ein ganz besondrer Saft

2001

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Functional properties of hemoglobin immobilized in coacervates prepared from gelatin A and polyanionic carbohydrates

Brouwer

Cashon

Bonaventura

1990

Biotech & Bioengineering

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Complex coacervation is a phenomenon of phase separation that may occur in a solution of positively and negatively charged polyions. The resulting two phases are distinguished by the total concentration of both polyions, with the concentrated phase often containing vesicular structures composed of the two polyelectrolytes. We have used this phenomenon in an attempt to-prepare a hemoglobin-based red blood cell analog. Hemoglobin-containing coacervate vesicles have been prepared from gelatin A and the polyanionic carbohydrates acacia, pectin, or dextranstilfate. Hemoglobin seems to be anchored into the vesicle walls through interaction of its polyanion binding site with the negatively charged residues on the carbohydrates. Oxygen binding by the immobilized HbA is reversible and cooperative, with p50 values at 20 degrees C of 2.8, 6, and 24 mm Hg for the acacia- (pH 7.5), pectin- (pH 6.6), and dextransulfate-(pH 6.6) derived coacervates. Kinetic studies on CO binding show that the rate of CO uptake by the coacervates (t((1/2)) = 13-27 ms at 0.5 mM CO) is similar to that of human erythrocytes.The HbA-containing coacervates slowly dissolve in isotonic salt solutions (145 mM NaCl, pH 7.4), but they can be stabilized by treatment with glutaraldehyde. Oxygen binding by HbA incorporated into the stabilized coacervates derived from dextran sulfate is very similar to oxy gen binding by human red blood cells: p50 = 26 mm Hg and n = 1.89 at 37 degrees C in isotonic salt. These results show how a novel approach, based on an old concept, has led to the preparation of immobilized HbA, with functional properties similar to those of intraerythrocytic HbA.

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Soluble functional polymers for human haemoglobin modification for blood substitution

et al. 1990

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Aqueous solutions of certain polymers have been used as plasma expanders for a long time. However, these polymer solutions are only capable of correcting the volume of circulation in vivo and in particular they cannot replace the oxygen‐carrying function of blood. This property can be conferred to these polymer solutions by linking human haemoglobin, the natural oxygen carrier, to the polymers in such a way that the oxygen‐binding properties of the free protein are similar to those of blood. Such results can be achieved by designing and preparing special polyanionic derivatives of dextran and polyoxyethylene and linking them to free human haemoglobin under its deoxy form or its oxy form. The resulting covalent conjugates exhibit general properties quite fitted to a use in blood substitution.

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Comparison of the Properties of Human Hemoglobin Covalently Bound to Carboxyl Dextrans with Free and Polymerized Hemoglobin

Cited by 3 publications

References 12 publications

Oxygen Carriers (“Blood Substitutes”)Raison d'Etre, Chemistry, and Some PhysiologyBlut ist ein ganz besondrer Saft

Oxygen Carriers (“Blood Substitutes”)Raison d'Etre, Chemistry, and Some PhysiologyBlut ist ein ganz besondrer Saft

Functional properties of hemoglobin immobilized in coacervates prepared from gelatin A and polyanionic carbohydrates

Soluble functional polymers for human haemoglobin modification for blood substitution

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