Comparison of the precursor, amino acid oxidation, and end-product methods for the evaluation of protein turnover in senior dogs
Letícia G. Pacheco,
Camila Goloni,
Ludmilla G. Di Santo
et al.
Abstract:Stable isotope methods have been used to study protein metabolism in humans; however, there application in dogs has not been frequently explored. The present study compared the methods of precursor (13C-Leucine), end-products (15N-Glycine), and amino acid oxidation (13C-Phenylalanine) to determine the whole-body protein turnover rate in senior dogs. Six dogs (12.7 ± 2.6 years age, 13.6 ± 0.6 kg bodyweight) received a dry food diet for maintenance and were subjected to all the above-mentioned methods in success… Show more
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