Comparison of the Polyphenolic Profile of Medicago sativa L. and Trifolium pratense L. Sprouts in Different Germination Stages Using the UHPLC-Q Exactive Hybrid Quadrupole Orbitrap High-Resolution Mass Spectrometry

Abstract

Identification and quantification of polyphenols in plant material are of great interest since they make a significant contribution to its total bioactivity. In the present study, an UPLC-Orbitrap-MS/MS approach using the variable data acquisition mode (vDIA) was developed and applied for rapid separation, identification, and quantification of the main polyphenolic compounds in Medicago sativa L. and Trifolium pratense L. sprouts in different germination stages. Based on accurate MS data and fragment ions identification strategy, a total of 29 compounds were identified by comparing their accurate masses, fragment ions, retention times, and literatures. Additionally, a number of 30 compounds were quantified by comparing to the reference standards. Data were statistically analysed. For both plant species, the sprouts of the third germination day are valuable sources of bioactive compounds and could be used in phytotherapy and nutrition. Although Trifolium pratense L. (Red Clover) is considered to be a reference for natural remedies in relieving menopause disorders, alfalfa also showed a high level of biological active compounds with estrogenic activity.

Extraction, Radical Scavenging Activities, and Chemical Composition Identification of Flavonoids from Sunflower (Helianthus annuus L.) Receptacles

“…The C3 chain of daidzein and isoquercetin were cleaved, the cleavage position has universality and particularity. In addition, the substitution patterns on the C6 rings lead to different types of produce ions and the intensity of relative abundances [ 41 , 42 ].…”

Effect of Elicitation with Iron Chelate and Sodium Metasilicate on Phenolic Compounds in Legume Sprouts

“…There are much less data on the composition and content of phenolic compounds in alfalfa sprouts. Earlier, it was found that alfalfa sprouts obtained in darkness contained glycosides of myricitin, morine, quercetin, and kaempferol [ 64 ]. In turn, data from another paper showed that alfalfa sprouts contain quercetin as the main flavonoid and low contents of myricetin, apigenin, and iso -rhamentin [ 65 ].…”

Advanced Analytical Approaches for the Analysis of Polyphenols in Plants Matrices—A Review

“…Regarding the methods' optimization, in the LC-HRMS/MS non-targeted screening analysis, resolution, scan rate and mass acquisition interval are the most critical parameters [99]. In addition to data acquisition modes also common in low-resolution spectrometry (SIM and MRM, PRM), new approaches such as data-dependent acquisition (DDA) and independent data acquisition (DIA) strategies, used so far only in proteomics, have recently been transposed into the analysis of small molecules in HRMS [110,114].…”

“…The limitation of the DDA strategy comes from the selection of precursors for MS/MS analysis [99]. Instead, in the recently developed independent data acquisition (DIA) strategy, all molecules within consecutive preselected m/z windows are subject to fragmentation, leading to higher specificity compared to the AIF (all ions fragmentation) approach [99,110]. As a disadvantage, the lack of precursor preselection can lead to impure mass spectra and low sensitivity.…”